IN VITRO EVALUATION OF SUN PROTECTION FACTOR (SPF) OF A CREAM FORMULATION PREPARED FROM EXTRACTS OF MUSA ACCUMINATA (L.), PSIDIUM GUJAVA (L.) AND PYRUS COMMUNIS (L.)

Authors

  • Shahlla Imam
  • Iqbal Azhar
  • Zafar Alam Mahmood Colorcon Limited - England

Abstract

Objectives: Use of phytoconstituents, especially obtained from fruits extract with high content of flavonoids has gained considerable importance
in personal care products such as creams and lotions. Finding new results and data through experiments will be helpful for both researchers and
industry on the subject. The purpose of this study was to evaluate in-vitro sunscreen activity of a cream formulation containing the fruit extract of
Musa accuminata, Psidium gujava and Pyrus communis based on their flavonoid contents.
Methods: Extraction of fruits to include maximum quantity of flavonoids was carried out using solvent system comprising of methanol (35%),
ethanol (35%), and distilled water (30%). The cream was formulated and tested for the physicochemical parameters such as color, odor, pH and
spreadability. While total flavonoid content was determined by aluminum chloride colorimetric method. The in-vitro sun protection factor (SPF) of
cream formulation and commercially available sunscreen was determined by ultraviolet spectrophotometric method.
Results: The total flavonoid content of cream formulation was found to be 45.81±8.49 and expressed in terms of standard quercetin equivalent
μg/g. The SPF value for the cream formulation was recorded as 3.90, whereas commercially available sunscreen it was 12.26, indicating that cream
formulation has photoprotective activity and may be used to develop a good cosmetic formulation and to explore its commercial viability.
Conclusion: Use of phytoconstituents, especially those obtained from fruits extract with high content of flavonoids has gained considerable
importance in personal care products such as creams and lotions. Finding new results and data through experiments will be helpful for both
researchers and industry on the subject. The proposed spectrophotometric method is simple and rapid for SPF determination. Due to the high cost
and time consumption relating to in vivo SPF determination andsome ethical issues for the volunteers, the in vitro method is gaining more importance.

Keywords: Antioxidants, Formulation, In-vitro sun protection factor, Photoprotection, Fruit extract, Ultraviolet-visible spectroscopy.

 

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Author Biography

Zafar Alam Mahmood, Colorcon Limited - England

Technical & Sales (Country Manager)

References

Balakrishnan KP, Narayanaswamy N. Review article. Botanicals as sunscreens: Their role in the prevention of photoaging and skin cancer. Int J Res Cosmet Sci 2011;1(1):1-12. Available from: http://www.urpjournals.com.

Karthika P, Jayshree N. Formulation and evaluation of sunscreen cream containing flower extract of Delonix Regia. Int J Pharm Integr Life Sci 2013;1(6):111-29. Available from: http://www.ijopils.com.

Sudhahar V, Balasubramanian V. Sun production factor (SPF) determination of marketed sunscreen formulation by in-vitro method using UV-VIS spectrophotometer. Arch Appl Sci Res 2013;5(6):119‑22. Available from: http://www.scholarsresearchlibrary.com/archive.html.

Kale S, Gaikwad M, Bhandare S. Determination and comparison of

Table 5: In‑vitro SPF value of commercial cream measured under different wavelength

S. No.

Wavelength (λnm)

EE×I (normalized)

Absorbance×CF×EE×I

SPF=ΣEE (λ)×I (λ)×Absorbance (A)×10

Labelled SPF

0150

1593±0.002

258103

0817

933831±0.001

2874

428682±0.001

3278

061442±0.002

1864

393376±0.003

0839

063852±0.003

018

21762±0.001

SPF: Sun protection factor

Table 6: SPF values and corresponding percentage of UV blockage

SPF

Percent of UV blocked

SPF: Sun protection factor, UV: Ultravioletin vitro SPF of topical formulation containing Lutein ester from Tagetes erecta L. flowers, Moringa oleifera Lam seed oil and Moringa oleifera Lam seed oil containing Lutein ester. Int J Res Pharm Biomed Sci 2011;2(3):1220-4. Available from: http://www.ijrpbsonline.com/.

Fonseca AP, Rafaela N. Determination of sun protection factor by UV-Vis spectrophotometry. Health Care Curr Rev 2013;1(1):108.

Pizzichini M, Arezzini L, Billarelli C, Carlucci F, Terzuoli L. Determination and separation of allantoin, uric acid, hypoxanthine and xanthine by capillary zone electrophoresis. In: Griesmacher A, Chiba P, Muller MM, editors. Purine and Pyrimidine Metabolism in Man IX. New York: Plenum Press; 1998. p. 797.

Anitha T. Review article; Medicinal plants used in skin protection. Asian J Pharm Clin Res 2012;5(3):35-38. Available from: http://www.ajpcr.com.

Svobodová A, Psotová J, Walterová D. Natural phenolics in the prevention of UV-induced skin damage. A review. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 2003;147(2):137-45.

Bambal V, Mishra M. Evaluation of in vitro sunscreen activity of herbal cream containing extract of Curcuma longa and Butea monosperma. World J Pharm Res 2014;3(2):3026-35. Available from: http://www.wjpr.net/.

Dutra EA, Oliveira DA, Kedor-Hackmann ER, Santoro MI. Determination of sun protection factor (SPF) of sunscreens by ultraviolet spectrophotometry. Braz J Pharm Sci 2004;40(3):381-5. Available from: http://www.revistas.usp.br/bjps.

Deore SL, Kombade S, Baviskar BA, Khadabadi SS. Review article photoprotective antioxidant phytochemicals. Int J Phytopharm 2012;2(3):72-6. Available from: http://www.ijpp.ssjournals.com/.

Mansur JS, Breder MN, Mansur MC, Azulay RD. Determination of sun protection factor by spectrophotometry. An Bras Dermatol 1986;61:121-4.

Sayre RM, Agin PP, LeVee GJ, Marlowe E. A comparison of in vivo and in vitro testing of sunscreening formulas. Photochem Photobiol 1979;29(3):559-66.

Kaur CD, Saraf S. In vitro sun protection factor determination of herbal oils used in cosmetics. Pharmacognosy Res 2010;2(1):22-5.

Purushotham RK, Khaliq K, Kharat SS, Sagare P, Patil SK. Preparation and evaluation o/w cream for skin psoriasis. Int J Pharm Bio Sci 2010;1(3):1-11. Available from: http://www.ijpbs.net.

Nabavi SM, Ebrahimzadeh MA, Nabavi SF, Hamidinia A, Bekhradnia AR. Determination of antioxidant activity, phenol and flavonoid content of Parrotia Persica mey. Pharmacologyonline 2008;2:560-7. Available from: http://www.pharmacologyonline.silae.it/front.

Imam S, Azhar I, Perveen S, Hussain SG, and Mahmood ZA. Studies on in vitro antioxidant activity and total Flavonoid contents of a cream formulation to correlate its anti-aging effect. World J Pharm Res 2015;4(1):1646-55. Available from: http://www.wjpr.net/.

Smaoui S, Hlima HB, Jarraya R, Kamoun NG, Ellouze R, Damak M. Cosmetic emulsion from virgin olive oil: Formulation and bio-physical evaluation. Afr J Biotech 2012;11(40):9664-967.Available from: http://www.academicjournals.org/AJB.

Saraf S, Sahu S, Kaur CD, Saraf S. Comparative measurement of hydration effects of herbal moisturizers. Pharmacognosy Res 2010;2(3):146-51.

Hassan SM, Al-Aqil AA, Attimarad M. Determination of crude saponin and total flavonoids content in guar meal. Adv Med Plant Res 2013;1(1):24-8. Available from: http://www.netjournals.org.

Available from: http://www.ottawand.com/articles-and-events/articles/sun-safety-for-summer/.

Saewam N, Jimtaisong A. Photoprotection of natural flavonoids. J Appl Pharm Sci 2013;3(9):129-41. Available from: http://www.japsonline.com.

Published

01-05-2015

How to Cite

Imam, S., I. Azhar, and Z. A. Mahmood. “IN VITRO EVALUATION OF SUN PROTECTION FACTOR (SPF) OF A CREAM FORMULATION PREPARED FROM EXTRACTS OF MUSA ACCUMINATA (L.), PSIDIUM GUJAVA (L.) AND PYRUS COMMUNIS (L.)”. Asian Journal of Pharmaceutical and Clinical Research, vol. 8, no. 3, May 2015, pp. 234-7, https://mail.innovareacademics.in/journals/index.php/ajpcr/article/view/5539.

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