DEVELOPMENT AND VALIDATION OF A REVERSED PHASE-PLANAR CHROMATOGRAPHY (HPTLC) METHOD FOR DETERMINATION OF BHT AND ITS APPLICATION IN FOOD PRODUCTS
Keywords:
BHT, RP-HPTLC, Densitometry, Planar chromatography, Food product, Method Development and ValidationAbstract
Objective: Development and validation of a RP-HPTLC method coupled with densitometry analysis for the determination of BHT as such and in commercial food sample (Butter). Methods: Chromatographic separation of BHT was achieved by employing a mobile phase consisting of Methanol – Water (9.5:0.5) on precoated aluminium TLC plates, silica gel RP-18 F254sand UV densitometric quantitative evaluation was performed by measuring the absorbance reflectance of the analyte spots at 254 nm. Results: The BHT was satisfactorily resolved with Rf value 0.48 ± 0.02. The study was carried out in the range of 500 ng to 5000 ng. The LOQ and LOD for BHT were 0.524 and 0.157 ng respectively. The method was validated with respect to linearity, repeatability, specificity, accuracy (recovery) and precision. Conclusion: The proposed method has high degree of repeatability and will provide fast and cost-effective quantitative control for routine analysis of BHT as single and in food sample.
Keywords: BHT, RP-HPTLC, Densitometry, Planar chromatography, Food product, Method Development and Validation
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