ANTIOXIDANT AND ANTIHEMOLYTIC ACTIVITY OF AN ENDANGERED PLANT SPECIES, HILDEGARDIA POPULIFOLIA (ROXB.) SCHOTT & ENDL.
Abstract
Objective: The present study was aimed at to estimate total content of ascorbic acid, saponins and condensed tannins and to evaluate the antioxidant activity and antihemolytic activity of methanolic extracts of leaf and stem bark parts of Hildegardia populifolia. Methods: Antioxidant activity was carried out by studying nitric oxide scavenging activity, inhibition of β – carotene bleaching activity and antihemolytic activity also studied. Results: Higher amount of ascorbic acid (93.47 g AA/100 g extract), saponins (18.81 g DE/100g extract) and condensed tannins (0.70 mg LE/g dry sample) was observed in methanolic leaf extract than that of the stem bark extract. Nitric oxide scavenging and β – carotene bleaching activity were relatively higher in stem bark extract (IC50 -122.60 and 56.67percent respectively). Leaf extract has higher percentage of antihemolytic activity than that of the stem bark extract.  Conclusion: The results of the study confirmed that the studied plant might indeed be potential sources of natural antioxidant and antihemolytic agents.
Key words: Antioxidant, antihemolytic activity, Hildegardia populifolia.
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Varaprasad B, Katikala PK, Naidu KC, Penumajji S. Antifungal activity of selected plant extracts against pytopathogenic fungi Aspergillus niger. Indian Journal of Science and Technology 2009; 2(4): 87-90.
Saradha M, Paulsamy S. Antibacterial activity of leaf and stem bark extracts of the endangered tree species, Hildegardia populifolia (Roxb.) Schott and Endl. (Sterculiaceae). Journal of Research in Antimicrobials 2012a; 1: 023-027.
Saradha M, Paulsamy S. In vitro antioxidant activity and polyphenol estimation of methanolic extract of endangered medicinal tree species, Hildegardia populifolia (Roxb.) Schott & Endl. International Journal of Phytomedicine 2012b; 4: 362-368.
Saradha M, Paulsamy S. Antinociceptive and antiinflammatory activities of stem bark of an endangered medicinal plant, Hildegardia populifolia (Roxb.) Schott and Endl. International Journal of Pharma and Bio Sciences 2013; 4(3): 30-36.
Saradha M. Development of propagation strategies and evaluation of medicinal properties for the endangered plant species, Hildegardia populifolia (Roxb.) Schott & Endl. (Sterculiaceae). Thesis submitted to Bharathiar University, Coimbatore. Tamilnadu, 2013.
Scalbert A, Manach C, Morand C, Remesy C, and Jimenez L. Dietary polyphenols and the prevention of diseases. Critical Reviews in Food Science and Nutrition 2005; 45(4): 287–306.
Gafner F, Msonthi JD, Hostettmann K. Molluscicidal saponins from Talinum tenuissimum Dinter. Helvet. Chim. Acta. 1985; 68: 555-558.
Klein BP, and Perry AK. Ascorbic acid and Vitamin A activity in selected vegetables from different geographical areas of the United States. J. Food Sci 1982; 47: 941-945.
Makkar HPS, Siddhuraju P, and Becker K. Plant Secondary Metabolites. 1st Edn. Humana Press, Totowa, New Jersey, USA; 2007. p. 130.
Porter LJ, Hrstich LN, and Chan BG. The conversion of procyanidins and prodelphinidins to cyanidin and delphinidin. Phytochemistry 1986; 25: 223–230.
Sreejayan N, and Rao MNA. Nitric oxide scavenging by curcuminoids. Journal of Pharmacyand Pharmacology 1997; 49: 105–107.
Taga MS, Miller EE, and Pratt DE. Chia seeds as a source of natural lipid antioxidants. Journal of the American Oil Chemists’ Society 1984; 61: 928-931.
Naim M, Gestener B, Bondi A, and Birk Y. Antioxidant and antihemolytic activities of soyabean isoflavones. Journal of Agricultural and Food Chemistry. 1976; 24: 1174-1177.
Roman L, Stanila A, and Stanila S. Bioactive compounds and antioxidant activity of Rosa canina L. biotypes from spontaneous flora of Transylvania. Chemistry Central Journal 2013; 7: 1-10.
Shi J, Arunasalam K, Yeung D, Kakuda Y, Mittal G, and Jiang Y. Saponins from Edible Legumes: Chemistry, Processing, and Health Benefits. Journal of Medicinal Food 2004; 7(1): 67-78.
Hernes PJ, and Hedges JI. Determination of condensed tannin monomers in environmental samples by capillary gas chromatography of acid depolymerization extracts. Anal. Chem 2000; 72: 5115-5124.
Zivkovic J, Mujic I, Zekovic Z, Nikolic G, Vidovic S, Mujic A. Extraction and analysis of condensed tannins in Castanea sativa Mill. Journal of Central Europeon Agriculture 2009; 10(3): 283-288.
Ricardo-da-Silva JM, Darmon N, Fernandez Y, Mitjavila S. Oxygen free radical scavenger capacity in aqueous models of different procyanidins from grape seeds. Journal of Agricultural and Food Chemistry 1991; 39: 1549-1552.
Hagerman AE, Riedl KM, Jones GA, Sovik KN, Ritchard NT, Hartzfeld PW, and Riechel TL. High molecular weight plant polyphenolics (tannins) as biological antioxidants. J. Agric. Food Chem 1998; 46: 1887-1892.
Gutierrez RMP, Luna HH, and Garrido SH. Antioxidant activity of Tagates erecta essential oil. J. Chil. Chem. Soc 2006; 51: 883-886.
Niki E. Antioxidants in relation to lipid peroxidation. Chemical Physical Lipids 1982; 44: 227-253.
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