IN VITRO PROPAGATION OF ARUNDINA GRAMINIFOLIA D. DON. HOCHR. – A BAMBOO ORCHID

Abstract

 Arundina graminifolia (D. Don)Hochr. Known as bamboo orchid and it is an endangered orchid (Jain and Sastry, 1980; Chowdhery and Murti, 2000).Here a protocol was established for in vitro propagation of bamboo orchid. For micropropagation different concentrations of growth regulators were used and nodal segments were used as explants.MS media supplemented with NAA at different concentration (0.5mg/L,1.0mg/L,1.5mg/L) and Kinetin (1.0mg/L,2.0mg/L,2.5mg/L) was used for Shoot proliferation. Two types of auxin (NAA, IAA) were used to know the effects of both auxin in root induction.MS media supplemented with 1.0mg/L NAA+ 2.5mg/L KN showed highest shoot proliferation with root length 3.50 cm. MS media when supplemented with 3.0mg/L IAA induce root with highest root length4.7cm. 87percent micropropagated plants survived after field transfer.

 

Key Words : Micropropagation, in vitro, growth regulators, Endangered.