NEUROPROTECTIVE EVALUATION OF LAGENARIA VULGARIS EXTRACT HYPOXIC NEUROTOXICITY INDUCED RATS
Abstract
ABSTRACT
Objective: The present study was aimed to evaluate the neuroprotective effects of Lagenaria vulgaris seed oil (LVSO) in hypoxic neurotoxicity induced
neurodegeneration in mice.
Methods: Hypoxia was induced by providing sodium nitrite (5 ml/kg) in drinking water to mice for 14 days and mice were evaluated for elevated
plus maze test. On 15th day, mice were sacrificed and brain was isolated for estimation of non-enzymatic antioxidants (glutathione [GSH]), enzymatic
antioxidants superoxide dismutase (SOD), catalase (CAT), total protein (TP) in the brain tissue, and histopathological study.
Results: Treatment with LVSO significantly prevented raise in levels of oxidant enzymes. CAT, GSH, SOD, and TP content have significantly restored in
LVSO treated groups whereas toxic group has shown a significant decrease in levels compare to control group. LVSO (0.5 mg/kg) has shown maximum
protection. The observed results leading to neurodegeneration is also confirmed by the histopathological differences between treatment and hypoxic
mice
Conclusion: LVSO ameliorated antihypoxic effects induced by sodium nitrite and this effect may be mediated by antioxidant mechanism, thereby
enhancing neuroprotection.
Keywords: Neuroprotective, Antioxidants, Hypoxia, Seed oil.
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Fig. 4: Histopathological observations of brain in hypoxic neurotoxicity in mice, (a) Control group, (b) hypoxic group, (c) Lagenaria vulgaris seed oil (LVSO) (0.25 g/kg), (d) LVSO (0.5 g/kg)
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