GAS CHROMATOGRAPHY-MASS SPECTROMETRY/MASS SPECTROMETRY ANALYSIS OF TERMINALIA CATAPPA L. NUT AND ANTIMICROBIAL ASSAY

Authors

  • KRISHNAVENI M
  • Krishna Kumari G
  • Kalaivani M
  • Ragina Banu C

Abstract

ABSTRACT
Objective: The present study was carried out with the main objective of studying Terminalia catappa nut phyto-constituents through GC-MS/MS, as
well as its antibacterial, antifungal activities.
Methods: The T. catappa nut collected was shade dried and extracted with ethanol, the obtained ethanol extract was used for the whole study- GCMS/
MS, antimicrobial activity.
Results: The results of GC-MS/MS showed 12 peaks. Among the 12 peaks obtained, the highest peak area percent of 48.58 for Propane, 1,1-diethoxyand
24.36% for t-Butyl hydrogen phthalate followed by 3-Isopropoxy-1,1,1,7,7,7-hexamethyl-3,5,5-tris (trimethylsiloxy) tetrasiloxane showing 12.80
as peak area percent and all the other compound was found to be moderate in peak area percent which was in the range of 3.52-1.10 except β-l-
Arabinopyranoside methyl, and Cyclopropanecarboxylic acid, oct-3-en-2-yl ester as its peak area percent was very low showing 0.08 and 0.13. The
antibacterial activity was good with Escherichia coli when compared to Staphylococcus aureus likewise, the antifungal activity was good with Candida
albicans on comparison with Aspergillus niger.
Conclusion: The naturally occurring non –nutrient plant chemicals called as phytochemicals especially phenolics – a natural antioxidant contained
in T. catappa nut would have contributed for its antibacterial, antifungal properties by interfering with the phospholipid bilayer of the cell membrane
and destroying the enzymes required for cellular processes ensuring its therapeutic potential. The variation in therapeutic potential depends on the
nutrient content of the soil and other climatic conditions. This confirms the need, validity for phytochemical characterization via analytical methods.
Keywords: Antimicrobial, Analytical, Gas chromatography-mass spectrometry/MS, Terminalia sp.

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Author Biography

KRISHNAVENI M

Department of Biochemistry

Senir scale

References

REFERENCES

Bauer AW, Perry DM, Kirby WM. Single-disk antibiotic-sensitivity testing of staphylococci; an analysis of technique and results. AMA Arch Intern Med 1959;104(2):208-16.

Krishnaveni M, Dhanalakshmi R. Phytonutrient analysis in Terminalia catappa fruit, flesh, nut, shell. Int J Curr Pharm Rev Res 2015;6(1):28‑35.

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Table 1: Components in ethanol extract of Terminalia catappa Nut

S.No.

RT

Name of the compound

MF

MW

PA%

74

Propane, 1,1‑diethoxy‑

C7H16O2

58

10

H‑Pyrazole, 4,5‑dihydro‑3‑methyl‑1‑propyl‑

C7H14N2

93

36

‑Isopropoxy‑1,1,1,7,7,7‑hexamethyl‑3,5,5‑tris (trimethylsiloxy) tetrasiloxane

C18H52O7Si7

80

72

Pentanoic acid

C5H10O2

87

91

β‑l‑Arabinopyranoside, methyl

C6H12O5

08

34

Dodecanoic acid, ethyl ester

C14H28O2

10

32

‑Methyloctahydrocoumarin

C10H16O2

81

20

t‑Butyl hydrogen phthalate

C12H14O4

36

31

Cyclopropanecarboxylic acid, oct‑3‑en‑2‑yl ester

C12H20O2

13

95

tert‑Butyl 2‑aminophenylcarbamate ditms

C17H32N2O2Si2

52

34

Stigmastan‑3,5‑diene

C29H48

30

14

Cholane‑5,20 (22)‑diene‑3b‑phenoxy

C30H42O

53

MF: Molecular formulae, MW: Molecular weight, PA: Peak area percent

Fig. 1: Chromatogram

Table 2: Antimicrobial activity of Terminalia catappa Nut

Plant part used

Microbes tested

Zone of inhibition (mm)

Terminalia catappa Nut.

Escherichia coli

Staphylococcus aureus

5

Aspergillus niger

Candida albicans

Asian J Pharm Clin Res, Vol 8, Issue 4, 2015, 168-170

Krishnaveni et al.

Krishnaveni M, Jasbin Shyni G, Dhanalakshmi R, Magesh P, Ponraj K, Lavanya K, et al. A preliminary study on phytoanalysis, antioxidant potential of Terminalia catappa L. fruit flesh. Int J Pharm Sci Rev Res 2014;28(1):83-7.

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Krishnaveni M, Krishna Kumari G, Ragina Banu C, Kalaivani M. GC-MS/MS analysis of phytochemicals in Terminalia catappa L, antimicrobial assay. Indo Am J Pharm Res 2015;5(3):1250-4.

Krishnaveni M, Magesh P. Air pollution tolerance index induced by biochemical components in plants. Int J Pharm and Pharm Sci 2014;6(5):362-4.

Krishnaveni M, Durairaj S, Madhiyan P, Amsavalli L, Chandrasekar R. Impact of air pollution in plants near thermal power plant, Mettur, Salem, Tamil Nadu, India. Int J Pharm Sci Rev Res 2013;20(2):173-7.

Krishnaveni M, Durairaj S, Madhiyan P, Amsavalli L, Chandrasekar R. In vitro free radical scavenging activity of aqueous leaf extract of plants near thermal power plant, Mettur, Salem. Int J Pharm Sci and Res 2013;4(9):3659-62.

Published

01-07-2015

How to Cite

M, K., K. Kumari G, K. M, and R. Banu C. “GAS CHROMATOGRAPHY-MASS SPECTROMETRY/MASS SPECTROMETRY ANALYSIS OF TERMINALIA CATAPPA L. NUT AND ANTIMICROBIAL ASSAY”. Asian Journal of Pharmaceutical and Clinical Research, vol. 8, no. 4, July 2015, pp. 168-70, https://mail.innovareacademics.in/journals/index.php/ajpcr/article/view/6279.

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