BIOREDUCTION OF SILVER NANOPARTICLES FROM AQUEOUS STEM EXTRACT OF CATHARANTHUS ROSEUS AND BACTERICIDAL EFFECTS.
Abstract
Objective: Silver nanoparticles (AgNPs) were synthesized using the stem extract of the Catharanthus roseus (L.) plant using the green method. The
extract from the stem of C. roseus plant was obtained using standard dry powder extraction method. The colorless silver nitrate solution was changed
into brown color after the addition of stem extract indicates the formation of AgNPs. The chemical compounds present in the stem extract were
acting as a reducing agent for the synthesis of AgNPs. Methods: The AgNPs were analyzed with the help of UV-visible spectrophotometer for initial
confirmation. Fourier transform infra-red spectroscopy (FT-IR) confirmed the presence of various phytochemicals such as carbohydrate, phenolic
compounds, flavonoids, saponin. and alkaloids which were responsible for both reduction and stabilizing the action of the silver nanoparticles.
Results: From the transmission electron microscopy analysis, it was confirmed that Ag-nanoparticles are in nanosize range between 40-50nm and
are mono dispersed of particle distribution. Conclusion: From the zone of inhibition, it was confirmed that the synthesized Ag NP from the C. roseus
stem was found to have a very high antimicrobial effect. From this study, it is clear that the AgNp can be used as a potential antimicrobial agent.
Keywords: Catharanthus roseus, Silver nanoparticles, Anti-microbial activities.
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