CHEMICAL COMPOSITION AND ANTIMICROBIAL ACTIVITY OF DITERPENE AND ESSENTIAL OILS OF Hedychium roxburghii BL. RHIZOME

Authors

  • Rika Hartati School of Pharmacy Bandung Institute of Technology
  • Asep Gana Suganda
  • Irda Fidrianny

Abstract

Objective: The objective of the present study were to isolate and determine diterpene compound and essential oils from H. roxburghii Bl. rhizome, and investigated those antimicrobial activity.

Methods: The essential oils was obtained by steam distillation method, the residual was then extracted by reflux with ethanol. The content of essential oils was analyzed by GC/MS method.  Ethanolic residual-distillation extract was concentrated then used to isolate compound 1 by vacuum liquid chromatography and centrifugal chromatography. It was characterized by infrared spectrophotometry, 1H-NMR, 13C-NMR, HSQC-NMR, HMBC-NMR and carbon coupling NMR. The antimicrobial activity of essential oils, ethanolic residual-distillation extract and compound 1 were carried out by microdilution method.

Results: The oils exhibited antimicrobial activity against Bacillus subtilis ATCC 6633 (MIC 1,750 μg/ml), Staphylococcus aureus ATCC 6538 (MIC 1,750 μg/ml), Escherichia coli ATCC 8939 (MIC 3,500 μg/ml), Pseudomonas aeruginosa ATCC 9027 (> 3,500 μg/ml) and Candida albicans ATCC 10231 (MIC 875 μg/ml). A phytochemical study of the rhizome essential oils of H. roxburghii Bl. were performed by GC/MS and the result showed that fenchyl acetate (45.85%) was the main component of the oils. Compound 1 was identified as diterpene compound, coronarin E. Coronarin E have not exhibited MIC at 512 μg/ml, however it showed inhibition profile against all of tested microbes.

Conclusion: The essential oils and ethanolic residual-distillation extract of H. roxburghii Bl. rhizome exhibited weak antimicrobial profile. Compound 1 was identified as diterpene compound, (coronarin E), it was exhibited weak antimicrobial activity, but showed inhibition profile against all of tested microbes.

 Keywords: Hedychium roxburghii, Zingiberaceae, antimicrobial, essential oils, coronarin E

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Author Biography

Rika Hartati, School of Pharmacy Bandung Institute of Technology

Pharmaceutical Biology Reasearch Group School of Pharmacy

References

Riswan S, Setyowati FM. Procedia of 2nd Symposium of Farm and

Zingiberaceae, South China Institute of Botany; 1996. p. 196-218.

He EY. Study on Hedychium coronarium Koenig’s edibility

and its pharmacological experiments. Lishizhen Med Med Res

;11(1):1077-8.

Weyerstahl P, Marschall H, Thefeld K, Subba GC. Constituents of the

essential oil from the rhizomes of Hedychium gardnerianum Roscoe.

Flavour Fragr J 1998;13:377-88.

Matsuda H, Morikawa T, Sakamoto Y, Toguchida I, Yoshikawa M.

Labdane-type diterpenes with inhibitory effects on increase in vascular

permeability and nitric oxide production from Hedychium coronarium.

Bioorg Med Chem 2002;10(8):2527-34.

Medeiros JR, Campos LB, Mendonça SC, Davin LB, Lewis NG.

Composition and antimicrobial activity of the essential oils from

invasive species of the Azores, Hedychium gardnerianum and

Pittosporum undulatum. Phytochemistry 2003;64(2):561-5.

Suresh G, Reddy PP, Babu KS, Shaik TB, Kalivendi SV. Two new

cytotoxic labdane diterpenes from the rhizomes of Hedychium

coronarium. Bioorg Med Chem Lett 2010;20(24):7544-8.

Morita H, Itokawa H. Cytotoxic and antifungal diterpenes from the

seeds of Alpinia galanga. Planta Med 1988;54(2):117-20.

Itokawa H, Morita M, Mihashi S. Labdane and bisnorlabdane type

diterpenes from Alpinia speciosa K. Schum. Chem Pharm Bull

;28(11):3452-4.

Itokawa H, Morita H, Katou I, Takeya K, Cavalheiro AJ, de Oliveira RC,

et al. Cytotoxic diterpenes from the rhizomes of Hedychium coronarium.

Planta Med 1988;54(24):311-5.

Asian J Pharm Clin Res, Vol 8, Issue 5, 2015, 222-227

Hartati et al.

Chimnoi N, Pisutjaroenpong S, Ngiwsara L, Dechtrirut D,

Chokchaichamnankit D, Khunnawutmanotham N, et al. Labdane

diterpenes from the rhizomes of Hedychium coronarium. Nat Prod Res

;22(14):1249-56.

Taveira FN, Oliveira AB, Souza Filho JD, Braga FC. Epimers of

labdane diterpenes from the rhizomes of Hedychium Coronarium J.

koenig. Braz J Pharmacog 2005;15(1):55-9.

Itokawa H, Morita H, Takeya K, Motidome M. Diterpenes from

rhizomes of Hedychium coronarium. Chem Pharm Bull (Tokyo)

;36:2682-4.

Jayaprakasam B, Vanisree M, Zhang Y, Dewitt DL, Nair MG. Impact

of alkyl esters of caffeic and ferulic acids on tumor cell proliferation,

cyclooxygenase enzyme, and lipid peroxidation. J Agric Food Chem

;54(15):5375-81.

Reddy PP, Rao RR, Shashidhar J, Sastry BS, Rao JM, Babu KS.

Phytochemical investigation of labdane diterpenes from the rhizomes

of Hedychium spicatum and their cytotoxic activity. Bioorg Med Chem

Lett 2009;19(21):6078-81.

Ho JC. Antimicrobial, mosquito larvicidal and antioxidant properties

of the leaf and rhizome of Hedychium coronarium. J Chin Chem Soc

;58(4):563-7.

Prakash B, Singh P, Kedia A, Dubey NK. Assessment of some

essential oils as food preservatives based on antifungal, antiaflatoxin,

antioxidant activities and in vivo efficacy in food system. Food Res Int

;49(1):201-8.

Clinical and Laboratory Standard Institute (CLSI). Methods for

Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow

Aerobically: Approved Standard M7-A6. 6th ed., Vol. 23. Wayne, PA:

National Committee for Clinical Laboratory Standards; 2009.

Gill AO, Delaquis P, Russo P, Holley RA. Evaluation of antilisterial

action of cilantro oil on vacuum packed ham. Int J Food Microbiol

;73(1):83-92.

Burt S. Essential oils: Their antibacterial properties and potential

applications in foods – A review. Int J Food Microbiol 2004;94(3):223-53.

Lambert RJ, Skandamis PN, Coote PJ, Nychas GJ. A study of the

minimum inhibitory concentration and mode of action of oregano

essential oil, thymol and carvacrol. J Appl Microbiol 2001;91(3):453-62.

Carson CF, Cookson BD, Farrelly HD, Riley TV. Susceptibility of

methicillin-resistant Staphylococcus aureus to the essential oil of

Melaleuca alternifolia. J Antimicrob Chemother 1995;35(3):421-4.

Cosentino S, Tuberoso CI, Pisano B, Satta M, Mascia V, Arzedi E, et al.

In-vitro antimicrobial activity and chemical composition of Sardinian

thymus essential oils. Lett Appl Microbiol 1999;29(2):130-5.

Published

01-09-2015

How to Cite

Hartati, R., A. G. Suganda, and I. Fidrianny. “CHEMICAL COMPOSITION AND ANTIMICROBIAL ACTIVITY OF DITERPENE AND ESSENTIAL OILS OF Hedychium Roxburghii BL. RHIZOME”. Asian Journal of Pharmaceutical and Clinical Research, vol. 8, no. 5, Sept. 2015, pp. 209-14, https://mail.innovareacademics.in/journals/index.php/ajpcr/article/view/7348.

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