ANTINEOPLASTIC AND ANTIOXIDANT ACTIVITIES OF ACORUS CALAMUS L ON SWISS ALBINO MICE BEARING DALTON'S ASCITES LYMPHOMA
Abstract
Objective: Ethnobotanical alternates for synthetic allopathic drugs are soon gaining importance in the treatment of diseases due to their fewer side
effects. Acorus calamus has been widely used globally due its richness in antioxidant phytoconstituents in the treatments yielding beneficial functions.
The present study details the in vivo anticancerous effect of A. calamus against Dalton's ascites lymphoma (DAL).
Methods: The extract obtained by soxhlet extraction was screened for antioxidants by the enzymatic and non-enzymatic methods to indicate any
inhibitory properties on DAL induced cells.
Results: Soxhlet extraction using organic solvents and water revealed methanol to yield a maximum percentage of the crude extract that was stored
for antioxidant and antineoplastic analysis. DAL induced tumor cells in mice revealed decreased levels of superoxide dismutase, catalase, glutathione
peroxidise, glutathione, vitamin C and also vitamin E on analysis of liver and kidney samples. The same organs, when analyzed on treatment with the
methanolic extract of A. calamus revealed a reversal of these decreased values to normal range.
Conclusion: These results thus indicate the potential use of A. calamus for anticancer studies although the main phytoconstituent in the extract
obtained is to be further analyzed.
Keywords: Acorus calamus, Dalton's ascites lymphoma, Anticancer, Phytoconstituents, Antioxidants.
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References
Pandey R, Maurya R, Singh G, Sathiamoorthy B, Naik S.
Immunosuppressive properties of flavonoids isolated from Boerhaavia
diffusa Linn. Int Immunopharmacol 2005;5(3):541-53.
La Vecchia C, Tavani A. Fruit and vegetables, and human cancer. Eur J
Cancer Prev 1998;7(1):3-8.
Murali A, Ashok P, Madhavan V. In vitro antioxidant activity and
HPTLC studies on the roots and rhizomes of Smilax zeylanica
(Smilacaceae). Int J Pharm Sci 2011;3(1):192-5.
Gonzales GF, Valerio LG Jr. Medicinal plants from Peru: A review of
plants as potential agents against cancer. Anticancer Agents Med Chem
;6(5):429-44.
Motley TJ. The ethnobotany of sweet flag, Acorus calamus (Araceae).
Econ Bot 1994;48:397-412.
Yang CS, Landau JM, Huang MT, Newmark HL. Inhibition of
carcinogenesis by dietary polyphenolic compounds. Annu Rev Nutr
;21:381-406.
Lahiri-Chatterjee M, Katiyar SK, Mohan RR, Agarwal R. A flavonoid
antioxidant, silymarin, affords exceptionally high protection against
tumor promotion in the SENCAR mouse skin tumorigenesis model.
Cancer Res 1999;59(3):622-32.
Shanker A, Singh SM, Sodhi A. Ascitic growth of a spontaneous
transplantable T cell lymphoma induces thymic involution 2. Induction
of apoptosis in thymocytes. Tumour Biol 2000;21(6):315-27.
Ozaslan M, Karagoz ID, Kilic IH, Guldur ME. Ehrlich ascites
carcinoma. Afr J Biotechnol 2011;10(13):2375-8.
Rahman K. Studies on free radicals, antioxidants, and co-factors. Clin
Interv Aging 2007;2(2):219-36.
Forman HJ, Fridovich I. Superoxide dismutase: A comparison of rate
constants. Arch Biochem Biophys 1973;158(1):396-400.
Crapo JD, Oury T, Rabouille C, Slot JW, Chang LY. Copper, zinc
superoxide dismutase is primarily a cytosolic protein in human cells.
Proc Natl Acad Sci U S A 1992;89(21):10405-9.
Robinson BH. The role of manganese superoxide dismutase in health
and disease. J Inherit Metab Dis 1998;21(5):598-603.
Balendiran GK, Dabur R, Fraser D. The role of glutathione in cancer.
Cell Biochem Funct 2004;22(6):343-52.
Pryor WA. The formation of free radicals and the consequences of their
reactions in vivo. Photochem Photobiol 1978;28(4-5):787-801.
De Duve C, Hayaishi O. Tocopherol, Oxygen and Biomembranes.
Amsterdam: Elsevier; 1978.
Miquel J, Oro J, Bensch KG, Johnson JE. Free Radicals in Biology.
Vol. III. New York: Academic Press; 1977. p. 133.
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