APOPTOSIS AND FLOWCYTOMETRIC STUDIES OF BAUHINIA VARIEGATA BARK EXTRACT

Abstract

Objectives: The present investigation is focused on in vitro anticancer activity of flavonoid rich fraction of Bauhinia variegata bark ethanolic extract (BBEE) on HeLa cell lines.

Method: The Ic₅₀ value of extract was determined by MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyl tetrazolium bromide) and SRB (sulforhodamine B) assay. The extract treated HeLa cell lines were subjected to apoptosis determination by ethidium bromide/Acridium orange fluorescent staining method. Flow cytometric analysis was also carried out to analyse the effect of plant extract on cell cycle progression.

Results: The Ic₅₀ value of the extract was found to be 191.5 μg/ml against HeLa cell lines by MTT and SRB assay. BBEE extract induced apoptic cell death in HeLa cell lines. The flow cytometric analysis on BBEE treated HeLa cell lines shows the arrest of cell cycle in G0/G1 phase.

Conclusion: The studies indicate cytotoxic activity of Bauhinia variegata bark extract on HeLa cell lines.

 

 

Keywords: Bauhinia variegata bark, HeLa cells, apoptosis, Flow cytometry, MTT, SRB