INVITRO ANTI-OXIDANT AND ANTICANCER ACTIVITY OF MURRAYA KOENIGII AGAINST HUMAN COLON CANCER HT- 29 CELL LINES

Abstract

Objective: The main aim of the study was to screen  the ethanolic extract of  Murraya koenigii  leaves for its invitro anti-oxidant and anticancer activity and its efficacy against H-29 Cell lines.

Methods: Ethanolic extract of Murraya koenigii  were prepared and assayed for the presence of  phytochemicals. In vitro antioxidant assay were performed by DPPH Radical and FRAP assay.The effect of ethanolic extracts on HT-29 cancer cell lines were evaluated by MTT colorimetric assay.

 

Results: The preliminary phytochemical screening of ethanolic extract showed the presence of significant secondary metabolites. The efficacy of Murraya koenigii against HT-29 cell line showed that the incubation of cancer cells reduced the viability of HT-29 cells and the dead cells were significantly increased with high extract concentration .Hence ethanolic extract of  Murraya koenigii exhibited  high cytotoxicity.

Conclusion : Even at very low  concentration Murraya koenigii showed high efficacy.In conclusion Murraya koenigii possess significant antioxidant activity and anticancer  activity.

 

Key words: Murraya koenigii,anti oxidant,anticancer,colon cancer,HT 29 cell lines.