IN VIVO INVESTIGATION OF HEPATOPROTECTIVE ACTIVITY OF ASTERACANTHA LONGIFOLIANEES. ON CCL INDUCED HEPATOTOXICITY IN WISTAR ALBINO RATS
Abstract
Objectives: This study was aimed to investigate the phytochemical constituents, antioxidant activity and hepatoprotective activity of Asteracantha
longifolia leaf extract.
Methods: Hepatotoxicity was induced in normal albino rats. Carbon tetrachloride (CCl
4
)
was administered orally at a dosage of 20 mg/kg body weight
in physiological saline for 1 day. Group 1 served as Control. Group 2 served as disease control (CCl
20 mg/kg body weight). Group 3 served as animal
treated with the aqueous extract A. longifolia (150 mg/kg body weight orally administered for 15 days). Group 4 served as animal treated with the
aqueous extract A. longifolia (300 mg/kg body weight orally administered for 15 days). Group 5 served as animal treated with standard silymarin
drug (20 mg/kg body weight orally administered for 15 days). After completed the experiment, the liver markers, lipid profile, and antioxidants were
analyzed.
4
Results: Phytochemical screening of aqueous extract of A. longifolia leaves indicates the presence of flavonoids, tannins, glycosides, phenol, steroids,
alkaloids, quinone, saponin, and coumarin. Supplementation of A. longifolia significantly restored the liver markers, lipid profile, and antioxidant
markers on in CCl
4
induced Wistar albino rats.
Conclusions: The results of our study showed that A. longifolia possess significant hepatoprotective and antioxidant activity, probably due to its
phytochemicals.
Keywords: Asteracantha longifolia, Phytochemicals, Carbon tetrachloride, Antioxidant.
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