EFFECT OF BUTYLATED HYDROXYANISOLE ON HYDROGEN PEROXIDE INDUCED OXIDATIVE STRESS ON CEREBRAL GLIOMA CELL LINE

Authors

  • RIHAN ANSARI
  • QUAISARPERWEEN
  • GAURAV KUMAR
  • JAYANAND
  • DURG VIJAY RAI

Abstract

Objective: Butylated hydroxyl anisole (BHA) has potential protective effects on hydrogen peroxide (H2O2) induced oxidative stress on cerebral glioma cell (BMG-1).The study was carried out to investigate the neuro-protective effects of BHA againstBMG-1 cerebral glioma cell in vitro.

Methods:In vitro cytotoxicity assay, Oxidative Stress Mediated Apoptosis, Determination of Oxidative Stress Indices by DCFH-DA, Effect of BHAon Extent of Lipid Peroxidation and Total GSH Levels Measurement was carried out on BMG-1 cerebral glioma cell using standard procedures.

Results: BHA was showing very less toxicity, it reached IC50 value approximately equal to 200μM in 24hr treatment, decreases the distorted morphologyof the BMG-1 cells, Significant reduction of apoptotic bodies, Oxidative Stress, Lipid Peroxidation Levels and significantly increases the levels of glutathione.

Conclusion: The present study indicates the BHA may be effective in both prevention and treatmentof brain and neurodegenerative disorders in which free radical processes give a modulatory effect.

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Published

01-02-2014

How to Cite

ANSARI, R., QUAISARPERWEEN, G. KUMAR, JAYANAND, and D. V. RAI. “EFFECT OF BUTYLATED HYDROXYANISOLE ON HYDROGEN PEROXIDE INDUCED OXIDATIVE STRESS ON CEREBRAL GLIOMA CELL LINE”. Asian Journal of Pharmaceutical and Clinical Research, vol. 7, no. 6, Feb. 2014, pp. 177-80, https://mail.innovareacademics.in/journals/index.php/ajpcr/article/view/875.