POTENTIAL ALPHA GLUCOSIDASE INHIBITOR FROM SELECTED ZINGIBERACEAE FAMILY
Abstract
ABSTRACT
Objective: This study aimed to evaluate Zingiberaceae family as potential sources of alpha-glucosidase inhibitors (AGIs).
Methods: A 16 species of selected Zingiberaceae family were macerated by ethanol 96%. The extracts were filtered and concentrated by rotary
evaporator. Phytochemical analysis carried out on the dry extract. AGI was assayed in 50 mM phosphate buffer at pH 6.8 with 5 mM p-nitrophenyl-αD-glucopyranoside
as
the substrate.
Alpha-glucosidase
activity
was
determined
by
measuring the release
of the yellow
p-nitrophenol
at 400
nm
using
a
spectrophotometer
UV-Vis,
and the percentage
of inhibition was
calculated.
Results and Conclusion: The Zingiberaceae family showed IC
against alpha-glucosidase varies range from 28.4 µg/ml to 269.2 µg/ml. Curcuma
longa L., Zingiber cassumunar Roxb., Curcuma heyneana Val. Et van zijp, Curcuma xanthorrhiza Roxb., Zingiber ottensii Val., showed the highest
inhibition against alpha-glucosidase, with IC
50
50
28.4, 49.0, 78.2, 78.9, and 79.0 µg/ml, respectively. The Zingiberaceae family is potential sources as an
alternative medicine to treated diabetes mellitus.
Keywords: Zingiberaceae, Alpha-glucosidase inhibitor, Diabetes mellitus, Phytochemistry content.
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References
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