ANTIBACTERIAL EFFECT OF FLAVONOIDS FROM PROPOLIS PRODUCED BY TRIGONA ON ATPASE ACTIVITY OF STREPTOCOCCUS MUTANS
DOI:
https://doi.org/10.22159/ijap.2017.v9s2.02Keywords:
flavonoids, propolis, antibacterial, ATPase activity, Streptococcus mutansAbstract
Objective: Caries are a major problem related to oral health among children. As it is an infectious disease, bacteria, especially Streptococcus mutans, play an important role in caries. Flavonoids have been known to have antibacterial effects, and ethanol, even at very low concentrations, reportedly has antibacterial effects and is widely used as a basic ingredient in drugs.
Aim: This study aims to analyze the antibacterial effects of different concentrations of propolis flavonoids and ethanol against S. mutans ATPase activity.
Methods: S. mutans cells were exposed to 0.5%, 0.25%, 0.1%, 0.075%, and 0.05% propolis flavonoids and then incubated in an anaerobic atmosphere for 48 h. The ATPase activity in these cells was then tested using a colorimetric assay.
Results: No significant difference was found in the average ATPase activity of S. mutans after treatment with various concentrations of propolis flavonoids and ethanol.
Conclusions: Thus, 0.5%, 0.25%, 0.1%, 0.075%, and 0.05% propolis flavonoids and 10% ethanol had similar effects against S. mutans ATPase activity. Further studies are necessary to evaluate the efficacy of flavonoids from Trigona spp. propolis.
Â
Downloads
References
Bagramian RA, Garcia-Godoy F, Volpe AR. The global increase in
dental caries. A pending public health crisis. Am J Dent 2009;22:3-8.
Lundeen TF, Roberson TM. In: Studervant CM, Roberson TM, editors. Cariology: The Lesion, Etiology, Prevention and Control. 3rd ed. St.Louis, Missouri: Mosby Inc.; 1995. p. 62.
Simon L. The role of Streptococcus mutans and oral ecology in the
formation of dental caries. Lethbridge Undergrad Res J 2007;2:1-6.
Abachi S, Lee S, Rupasinghe HP. Molecular mechanisms of inhibition of Streptococcus species by phytochemicals. Molecules 2016;21. pii: E215.
Hasan S, Singh K, Danisuddin M, Verma PK, Khan AU. Inhibition of
major virulence pathways of Streptococcus mutans by quercitrin and
deoxynojirimycin: A synergistic approach of infection control. PLoS
One 2014;9:e91736.
Lachenmeier DW. Safety evaluation of topical applications of ethanol on the skin and inside the oral cavity. J Occup Med Toxicol 2008;3:26.
Rodrigues F, Lehmann M, do Amaral VS, Reguly ML, de Andrade HH. Genotoxicity of three mouthwash products, cepacol, periogard,
and plax, in the Drosophila wing-spot test. Environ Mol Mutagen
;48:644-9.
Pujirahayu N, Ritonga H, Uslinawaty Z. Properties and flavonoids
content in propolis of some extraction method of raw propolis. Int J
Pharm Pharm Sci 2014;6:338-40.
Kumar S, Pandey AK. Chemistry and biological activities of flavonoids: An overview. ScientificWorldJournal 2013;2013:162750.
Sharief N, Veni AS, Umamrao V. Quantification of phytochemical and antibacterial activity of fruit extract od Avicennia officialis. Asian J
Pharm Clin Res 2014;7:10-3.
Utama A, Shimizu H, Hasebe F, Morita K, Igarashi A, Shoji I, et al. Role of the DExH motif of the Japanese encephalitis virus and hepatitis C virus NS3 proteins in the ATPase and RNA helicase activities. Virology 2000;273:316-24.
Lemos JA, Burne RA. A model of efficiency: Stress tolerance by Streptococcus mutans. Microbiology 2008;154:3247-55.
Bogdanov S. Propolis: Composition, health, medicine: A review. Bee Prod Sci 2016;6:1-42.
Jayshree N, Narayanan N, Sriram L. Antibacterial, antifungal and
antimycrobacterial studies on some synthetic dimethoxy flavones.
Asian J Pharm Clin Res 2012;5:2011-3.
Suseno D. Aktivitas Antibakteri Propolis Trigona spp. Pada Dua
Konsentrasi Berbeda Terhadap Cairan Rumen Sapi, Biokimia, Program Studi Mipa, Institut Pertanian Bogor; 2009. p. 6-8.
Chatterjee I, Somerville GA, Heilmann C, Sahl HG, Maurer HH,
Herrmann M. Very low ethanol concentrations affect the viability
and growth recovery in post-stationary-phase Staphylococcus aureus
populations. Appl Environ Microbiol 2006;72:2627-36.
Bowden GH. Nutritional influences on biofilm development. Adv Dent Res 1997;11:81-99.
Wagh VD. Propolis: A wonder bees product and its pharmacological potentials. Adv Pharmacol Sci 2013;2013:308249.
Poór M, Veres B, Jakus PB, Antus C, Montskó G, ZrÃnyi Z, et al.
Flavonoid diosmetin increases ATP levels in kidney cells and relieves
ATP depleting effect of ochratoxin A. J Photochem Photobiol B
;132:1-9
Published
How to Cite
Issue
Section
