PROTECTIVE EFFICACY OF SILVER NANOPARTICLES SYNTHESIZED FROM SILYMARIN ON CISPLATIN INDUCED RENAL OXIDATIVE STRESS IN ALBINO RAT
DOI:
https://doi.org/10.22159/ijap.2018v10i5.28023Keywords:
Silymarin, Cisplatin, Silver Nanoparticles, Nephrotoxicity, HistopathologyAbstract
Objective: This present study was carried out to evaluate the protective efficacy of silver nanoparticles synthesized from silymarin on cisplatin-induced renal oxidative stress in the albino rat.
Methods: Silver nanoparticles of silymarin was characterized by particle size, UV-Visible, FTIR and XRD analysis. Albino rats were divided randomly into six groups of six animals each. Group I Normal rats were treated with an oral dose of distilled water for 15 d. Group II rats were treated with single i. p. dose of cisplatin (16 mg/kg) on day 1. Group III rats were treated only with oral dose of silymarin (50 mg/kg/d) for 15 d. Group IV rats were treated only with oral dose of silver nanoparticles of silymarin (50 mg/kg/d) for 15 d. Group V rats were treated with an oral dose of silymarin for 14 d after single i. p. dose of cisplatin on day 1. Group VI rats were treated with oral dose of silver nanoparticles of silymarin for 14 d after single i. p. dose of cisplatin on day 1. Collected blood samples and kidney tissue samples were used for biochemical, enzymatic antioxidant and histopathological studies in all groups.
Results: The formation of silver nanoparticles of silymarin were confirmed by particle size, UV-Visible analysis, crystalline nature was confirmed by X-ray diffraction analysis, and active principles were confirmed by FTIR analysis. Biochemical results of silver nanoparticles of silymarin-treated groups showed the significant (p˂0.05) decrease in the level of creatinine, urea, and uric acid as compared to cisplatin-induced rats and The enzymatic antioxidants of silver nanoparticles of silymarin-treated groups showed the significant (p˂0.05) increase in the level of glutathione reductase and significant decrease in MDA level as compared to cisplatin-induced rats. Additionally, histopathological results of silver nanoparticles of silymarin-treated groups also confirmed that the ameliorative effect of silymarin nanoparticle against cisplatin-induced rats.
Conclusion: From this research work, we have concluded that silver nanoparticles of silymarin protected the kidney of albino rats from the adverse effects caused by cisplatin.
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