THE AUTHENTICATION OF VIRGIN COCONUT OIL FROM GRAPE SEED OIL AND SOYBEAN OIL USING FTIR SPECTROSCOPY AND CHEMOMETRICS

ABDUL ROHMAN; YAAKOB BIN CHE MAN; MD. EAKUB ALI

doi:10.22159/ijap.2019v11i2.31758

Authors

ABDUL ROHMAN Research Center of Halal Products, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.
YAAKOB BIN CHE MAN Halal Products Research Institute, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.
MD. EAKUB ALI Nanotechnology and Catalysis Research Centre (NanoCat), University of Malaya, Kuala Lumpur 50603, Malaysia

DOI:

https://doi.org/10.22159/ijap.2019v11i2.31758

Keywords:

Virgin coconut oil, Chemometrics, Authentication, FTIR spectroscopy

Abstract

Objective: The objective of this study was to develop Fourier transform infrared (FTIR) spectroscopy in combination with chemometrics of multivariate calibration and discriminant analysis (DA) for the authentication of virgin coconut oil (VCO) from grape seed oil (GSO) and soybean oil (SO).

Methods: FTIR spectra of VCO, GSO, SO and its binary mixture of VCO-SO, and VCO-GSO were scanned at mid-infrared region (4000-650 cm^-1) using attenuated total reflectance technique. The wavenumbers were selected based on its capability to provide the best prediction models for quantification and classification of adulterants in VCO assisted by multivariate calibrations and DA, respectively.

Results: The results showed that partial least square (PLS) calibration using absorbance values at combined wavenumbers of 1200-900 and 3027-2985 cm^-1 revealed reliable method for quantification of GSO in VCO, as indicated by high value of coefficient of determination (R²) and low value of root mean square of calibration (RMSEC) and root mean square error of prediction (RMSEP). PLS using FTIR spectra at the combined wavenumbers of 1200-1000 and 3025-2995 cm^-1 was suitable for quantitative analysis of SO in VCO. DAwas also successfully used for classification of VCO and VCO added with adulterants of GSO and SO.

Conclusion: FTIR spectroscopy in combination with chemometrics of multivariate calibration and DA offered effective tools for the authentication of VCO