EFFECT OF HONEY PROPOLIS HARD CANDY ON LACTOPEROXIDASE ACTIVITY IN UNSTIMULATED SALIVA
DOI:
https://doi.org/10.22159/ijap.2019.v11s1.159Keywords:
Propolis, Lactoperoxidase, SalivaAbstract
Objective: We determined the effectiveness of honey propolis hard candy on salivary lactoperoxidase activity in unstimulated saliva.
Methods: In this pretest-posttest laboratory experimental design study, saliva collected from 120 subjects was reacted with potassium iodide,
phosphate buffer, and hydrogen peroxide. Salivary lactoperoxidase activity was calculated by absorbance value using a microplate reader at
wavelength of 340 nm.
Results: Honey propolis hard candy increased salivary lactoperoxidase activity, although the increase was not statistically significant (p>0.05).
Conclusions: Honey propolis hard candy tends to increase salivary lactoperoxidase activity.
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References
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mechanism and implication about antioxidant capacity. Chem Res
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of human saliva: Requirements for accumulation of hypothiocyanite.
J Dent Res 1981;60:785-96.
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Sigusch BW, et al. Lipid peroxidation and antioxidant activity in saliva
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hypothiocyanite (OSCN-) ion in human parotid saliva and the effect
of pH on OSCN-generation in the salivary peroxidase antimicrobial
system. Arch Oral Biol 1983;28:517-25.
12. Lamberts BL, Pruitt KM, Pederson ED, Golding MP. Comparison of
salivary peroxidase system components in caries-free and caries-active
naval recruits. Caries Res 1984;18:488-94.
13. Alexander A. Prevention of Oral Health Diseases with LP-System [LPsystem
Cegah Penyakit Gigi dan Mulut]; 2003. Available from: http://
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14. Benbahouche Nel H, Iliopoulos I, Török I, Marhold J, Henri J,
Kajava AV, et al. Drosophila spag is the homolog of RNA polymerase
II-associated protein 3 (RPAP3) and recruits the heat shock proteins 70
and 90 (Hsp70 and hsp90) during the assembly of cellular machineries.
J Biol Chem 2014;289:6236-47.
15. Malesic J, Kolar J, Strlic M, Polanc S. The influence of halide and
pseudo-halide antioxidants in fenton-like reaction systems. Acta Chim
Slov 2006;53:450-6.
16. Thomas EL, Bates KP, Jefferson MM. Hypothiocyanite ion: Detection
of the antimicrobial agent in human saliva. J Dent Res 1980;59:1466?72.
17. Mäkinen KK, Tenovuo J, Scheinin A. Xylitol-induced increase of
lactoperoxidase activity. J Dent Res 1976;55:652-60.
18. Aisyiah S. Analysis of Effect of Water Gargles of Betel Leaves for 120
Seconds on Saliva Peroxidase Activity (with Comparison of 32 Grams
of Betel Leaf and 100 ml of Water). [Dissertation]. Jakarta; 2006.
19. Manisha M. Honey: Its medicinal property and antibacterial activity.
Asian Pac J Trop Biomed 2011;1:154-60.
20. Kumar S, Debjit B. Medicinal uses and health benefits of honey: An
overview. J Chem Pharmacol 2010;2:385-95.
21. Setsu E. Cariogenicity of polydextrose and refined polydextrose as a
substrate. Nichidai Koko Kagaku 1989;15:1-11.
22. Soekanto SA, Marpaung LJ, Himmatushohwah, Djais A, Darwita RR.
Efficacy of Propolis Fluoride and Nano Silver Fluoride for Inhibition of
Streptococcus mutans and Enterococcus faecalis Biofilm Formation Int
J App Pharm 2017;2:51-4.
23. Soekanto SA, Ghrena GCD, Bachtiar EW, Sahlan M. The Effect Of
Casein Phosphopeptide-Amorphous Calcium Phosphate-Propolis
Chewing Gum On Calcium and Phosphate Ion Levels in Caries-Free
Subject’s Saliva and Streptococcus Mutans Biofilm Formation. Asian J
Pharm Clin Res. 2017;174-6.
Indonesia. Indonesia Oral Health Care Profile. Jakarta: Ministry of
Health, Republic of Indonesia; 2014.
2. Coutinho A. Honeybee propolis extract in periodontal treatment:
A clinical and microbiological study of propolis in periodontal
treatment. Indian J Dent Res 2012;23:294.
3. Wagh VD. Review Article Propolis: A Wonder Bees Product and Its
Pharmacological Potentials. Advances in Pharmacological Sciences
Volume 2013, Article ID 308249, 1-11.
4. Khalil ML. Biological activity of bee propolis in health and disease.
Asian Pac J Cancer Prev 2006;7:22-31.
5. Tsai YC, Wang YH, Liou CC, Lin YC, Huang H, Liu YC, et al.
Induction of oxidative DNA damage by flavonoids of propolis: Its
mechanism and implication about antioxidant capacity. Chem Res
Toxicol 2012;25:191-6.
6. Burdock GA. Review of the biological properties and toxicity of bee
propolis (propolis). Food Chem Toxicol 1998;36:347-63.
7. Gladea Z. The Effect of Consumption of Honey Propolis Candy on the
Prevalence of Streptococcus mutans in Caries Patients. [Dissertation].
Jakarta; 2012.
8. Ihalin R, Loimaranta V, Tenovuo J. Origin, structure, and biological
activities of peroxidases in human saliva. Arch Biochem Biophys 2006;
445:261-8.
9. Thomas EL, Bates KP, Jefferson MM. Peroxidase antimicrobial system
of human saliva: Requirements for accumulation of hypothiocyanite.
J Dent Res 1981;60:785-96.
10. Guentsch A, Preshaw PM, Bremer-Streck S, Klinger G, Glockmann E,
Sigusch BW, et al. Lipid peroxidation and antioxidant activity in saliva
of periodontitis patients: Effect of smoking and periodontal treatment.
Clin Oral Investig 2008;12:345-52.
11. Pruitt KM, Mansson-Rahemtulla B, Tenovuo J. Detection of the
hypothiocyanite (OSCN-) ion in human parotid saliva and the effect
of pH on OSCN-generation in the salivary peroxidase antimicrobial
system. Arch Oral Biol 1983;28:517-25.
12. Lamberts BL, Pruitt KM, Pederson ED, Golding MP. Comparison of
salivary peroxidase system components in caries-free and caries-active
naval recruits. Caries Res 1984;18:488-94.
13. Alexander A. Prevention of Oral Health Diseases with LP-System [LPsystem
Cegah Penyakit Gigi dan Mulut]; 2003. Available from: http://
www.kompas.com.
14. Benbahouche Nel H, Iliopoulos I, Török I, Marhold J, Henri J,
Kajava AV, et al. Drosophila spag is the homolog of RNA polymerase
II-associated protein 3 (RPAP3) and recruits the heat shock proteins 70
and 90 (Hsp70 and hsp90) during the assembly of cellular machineries.
J Biol Chem 2014;289:6236-47.
15. Malesic J, Kolar J, Strlic M, Polanc S. The influence of halide and
pseudo-halide antioxidants in fenton-like reaction systems. Acta Chim
Slov 2006;53:450-6.
16. Thomas EL, Bates KP, Jefferson MM. Hypothiocyanite ion: Detection
of the antimicrobial agent in human saliva. J Dent Res 1980;59:1466?72.
17. Mäkinen KK, Tenovuo J, Scheinin A. Xylitol-induced increase of
lactoperoxidase activity. J Dent Res 1976;55:652-60.
18. Aisyiah S. Analysis of Effect of Water Gargles of Betel Leaves for 120
Seconds on Saliva Peroxidase Activity (with Comparison of 32 Grams
of Betel Leaf and 100 ml of Water). [Dissertation]. Jakarta; 2006.
19. Manisha M. Honey: Its medicinal property and antibacterial activity.
Asian Pac J Trop Biomed 2011;1:154-60.
20. Kumar S, Debjit B. Medicinal uses and health benefits of honey: An
overview. J Chem Pharmacol 2010;2:385-95.
21. Setsu E. Cariogenicity of polydextrose and refined polydextrose as a
substrate. Nichidai Koko Kagaku 1989;15:1-11.
22. Soekanto SA, Marpaung LJ, Himmatushohwah, Djais A, Darwita RR.
Efficacy of Propolis Fluoride and Nano Silver Fluoride for Inhibition of
Streptococcus mutans and Enterococcus faecalis Biofilm Formation Int
J App Pharm 2017;2:51-4.
23. Soekanto SA, Ghrena GCD, Bachtiar EW, Sahlan M. The Effect Of
Casein Phosphopeptide-Amorphous Calcium Phosphate-Propolis
Chewing Gum On Calcium and Phosphate Ion Levels in Caries-Free
Subject’s Saliva and Streptococcus Mutans Biofilm Formation. Asian J
Pharm Clin Res. 2017;174-6.
Published
05-04-2019
How to Cite
WIJAYA, K. M., SOEKANTO, S. A., & SOEDARSONO, N. (2019). EFFECT OF HONEY PROPOLIS HARD CANDY ON LACTOPEROXIDASE ACTIVITY IN UNSTIMULATED SALIVA. International Journal of Applied Pharmaceutics, 11(1), 103–105. https://doi.org/10.22159/ijap.2019.v11s1.159
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