THE EFFECT OF MOUTHWASH COMBINATION OF IMMUNOGLOBULIN-Y ANTI-COMD STREPTOCOCCUS MUTANS AND CHITOSAN ON THE FORMATION OF STREPTOCOCCUS MUTANS BIOFILM

ENDANG WINIATI BACHTIAR; DETA APRITANTIA; AGOENG TJAHAJANI SARWONO; BOY M. BACHTIAR; RETNO D. SOEJOEDONO; I. WAYAN TEGUH WIBAWAN

doi:10.22159/ijap.2019.v11s1.AR170

Authors

ENDANG WINIATI BACHTIAR Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia.
DETA APRITANTIA Oral Science Research Center, Faculty of Dentistry Universitas Indonesia, Jakarta, Indonesia.
AGOENG TJAHAJANI SARWONO Oral Science Research Center, Faculty of Dentistry Universitas Indonesia, Jakarta, Indonesia.
BOY M. BACHTIAR Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia.
RETNO D. SOEJOEDONO Faculty of Veterinary Institute Pertanian Bogor, Indonesia.
I. WAYAN TEGUH WIBAWAN Faculty of Veterinary Institute Pertanian Bogor, Indonesia.

DOI:

https://doi.org/10.22159/ijap.2019.v11s1.AR170

Keywords:

Biofilm, Chitosan, IgY, Streptococcus mutans, Mouthwash

Abstract

Objective: The objective of this study was to investigate the effectiveness of immunoglobulin Y (IgY) antibodies as a dental caries vaccine by utilizing
IgY specific to the quorum-sensing signaling receptor ComD of Streptococcus mutans combined with chitosan in the form of mouthwash.
Methods: The effects of a mouthwash containing IgY anti-ComD S. mutans with and without chitosan on biofilm-forming isolates of S. mutans were
investigated. Subjects were assigned to rinsing twice daily for 6 days with 15–20 ml mouthwash solution for 30 s. Biofilm formation of S mutans
isolated from the patiens was measured using a crystal violet method to determine the optical density at 490 nm.
Results: The results indicated that mouthwash containing IgY anti-ComD S. mutans and chitosan significantly enhanced the biofilm formation of
S. mutans. In mouthwash containing IgY anti-ComD S. mutans without chitosan, a reduction in biofilm formation was observed; however, this was not
statistically significant.
Conclusions: The mouthwash combination of IgY anti-ComD S. mutans and chitosan enhanced the biofilm formation ability of S. mutans isolated from
caries and caries-free subjects. Further, research is needed to determine the appropriate concentrations of IgY anti-ComD S. mutans and chitosan
required to effectively inhibit dental caries.

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