DIFFERENCES IN THE POTENTIAL MUTAGENICITY OF RESIN-, SILICONE-, AND BIOCERAMIC-BASED SEALERS ON LYMPHOCYTES: A PROTEIN EXPRESSION ANALYSIS

SANI SURYADARMA; KAMIZAR; ENDANG SUPRASTIWI; RATNA MEIDYAWATI; MAIDA FITRI

doi:10.22159/ijap.2019.v11s1.16021

Authors

SANI SURYADARMA Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia
KAMIZAR Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia
ENDANG SUPRASTIWI Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia
RATNA MEIDYAWATI Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia
MAIDA FITRI Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia

DOI:

https://doi.org/10.22159/ijap.2019.v11s1.16021

Keywords:

Root canal sealers, Bioceramic, Protein expression, Lymphocyte, Mutagenicity, Resin, Silicone

Abstract

Objective: The objective of this study was to compare the potential mutagenicity of resin-, silicone-, and bioceramic-based sealers on protein
expression in human lymphocytes. There has been limited research on resin-, silicone-, and bioceramic-based sealers effects on protein expression
in lymphocytes.
Methods: Nine samples of each sealer were incubated in 2 mL human blood for 1, 3, and 7 days. Then, the isolated lymphocytes are observed for
protein separation by electrophoresis method. Profile of protein bands observed and data were analyzed statistically by Kruskal–Wallis and post hoc
Mann–Whitney.
Results: Although no statistically significant differences in protein bands were observed among the resin-, silicone-, and bioceramic-based sealers
(p=0.111), there was a statistically significant difference between the resin- and silicone-based sealers on the 1st day (p=0.046) and 3rd day (p=0.046)
and between the silicone- and bioceramic-based sealers on the 1st day (p=0.046). Thus, the present study shows that there were differences in the
potential mutagenicity on the 1st day; resin was potentially more mutagenic followed by bioceramic and silicone. On the 3rd and 7th days, bioceramic
was potentially more mutagenic followed by resin and silicone.
Conclusion: The manuscript describes the study in detail and concludes that resin was potentially more mutagenic followed by bioceramic- and
silicone-based sealers.

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