EFFECT OF TRANSFERSOME ON THE STABILITY AND ANTIOXIDANT ACTIVITY OF GLUTATHIONE IN ANTIAGING CREAMS

HARMITA HARMITA; ISKANDARSYAH; FRIAINI ZAHRA MURTI

doi:10.22159/ijap.2020.v12s1.FF033

Authors

HARMITA HARMITA Department of Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia
ISKANDARSYAH Department of Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia
FRIAINI ZAHRA MURTI Department of Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia

DOI:

https://doi.org/10.22159/ijap.2020.v12s1.FF033

Keywords:

Glutathione, Transfersome, Cream, High-performance liquid chromatography, Optimization, Stability test, Antioxidant activity test, Penetration test

Abstract

Objective: Glutathione is an important antioxidant compound that is added to various cosmetic preparations. This study compared the stability,
antioxidant activity, and penetration of glutathione creams formulated with and without transfersome, a commonly used carrier system.
Methods: The particle size of the transfersome was 55.65 nm, with a polydispersity index of 0.398 and an entrapment efficiency of 66.22%. During
cycling and centrifugal testing, the creams (with and without transfersome) did not change color or demonstrate phase separation. Chemical stability
analyses of the products were performed using high-performance liquid chromatography.
Results: The remaining glutathione content in the transfersome cream was 83.44%, while that of the non-transfersome cream was 47.92%. In the
penetration test using Franz diffusion cells, the transfersome cream demonstrated a cumulative penetration of 4474.44 μg/cm2, with a cumulative
concentration percentage of 39.60% and a flux of 510.38 μg/cm−2h−1. In contrast, the non-transfersome cream demonstrated a cumulative penetration
amount of 2793.80 μg/cm2, with a cumulative concentration percentage of 24.73% and a flux of 340.12 μg/cm−2h−1. In addition, the IC50 value of the
transfersome cream preparation was 11.89 μg/mL, while that of the non-transfersome preparation was 15.57 μg/mL.
Conclusion: Our findings indicate that the use of transfersome increases the stability and penetration of glutathione in cream preparations.

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