RESPONSE SURFACE METHODOLOGY APPLIED TO OXALIC ACID HYDROLYSIS OF OIL PALM EMPTY FRUIT BUNCH BIOMASS FOR D-XYLOSE PRODUCTION
DOI:
https://doi.org/10.22159/ijap.2020.v12s1.FF037Keywords:
D-Xylose, Oxalic acid, Oil palm empty fruit bunch, Hydrolysate, Response surface methodology, Acid hydrolysisAbstract
Objective: The study aimed to identify the best conditions using oxalic acid for hydrolysis of hemicellulose in oil palm empty fruit bunch (OPEFB)
biomass.
Methods: The analytical method of high-performance liquid chromatography (HPLC) was using a SUPELCOSIL LC-NH2 column, refractive index
detection detector, and three compositions of the mobile phase. At first, the hydrolysis of hemicellulose in OPEFB powder was optimized by applying
a response surface methodology. A three-variable, six-central composite design was used for the experiments. Temperature (between 95°C and
135°C), reaction time (between 10 and 110 min), and oxalic acid concentration (between 1% and 7% [w/v]) were evaluated by running 15 different
experiments at constant biomass concentrations. Then, hydrolysis was optimized again at the constant temperature selected with three variables:
OPEFB concentration, reaction time, and oxalic acid concentration. Hydrolysate samples were detoxified with carbon active, and furfural compound
was analyzed by gas chromatography with flame ionization detector.
Results: The optimum condition of HPLC was using acetonitrile: water (9:1) at a flow rate of 1.0 ml/min. The first hydrolysis results showed
a high yield of D-xylose produced, which was 6.40 g D-xylose/100 g OPEFB biomass, with a xylose recovery of 93.8%. However, this result was
not yet optimum. Further hydrolysis at constant temperature experiment produced the highest xylose yield of 13.13%, equivalent to 32 g/l
D-xylose.
Conclusion: The yield of D-xylose from mild hydrolysis using oxalic acid was similar to that using dilute sulfuric acid as used in the previous study
by Rahman et al.
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