MITRAGYNINE PERCENTAGES OF VARIOUS KRATOM VARIANTS SEIZED IN INDONESIA: A QUANTITATIVE ANALYSIS USING LIQUID CHROMATOGRAPHY-PHOTO DIODE ARRAY DETECTOR

TANTI; CHRISTIEN ANDRIYANI LALANGI; ERI ARFIYANI; WIDIANTI NINGTIAS; ERLANA NINDYA MAULIDA

doi:10.22159/ijap.2021v13i5.41910

Authors

TANTI Quality and Research Division, the Center of Narcotics Laboratory of National Narcotics Board (BNN) of the Republic of Indonesia
CHRISTIEN ANDRIYANI LALANGI Quality and Research Division, the Center of Narcotics Laboratory of National Narcotics Board (BNN) of the Republic of Indonesia
ERI ARFIYANI Quality and Research Division, the Center of Narcotics Laboratory of National Narcotics Board (BNN) of the Republic of Indonesia
WIDIANTI NINGTIAS Quality and Research Division, the Center of Narcotics Laboratory of National Narcotics Board (BNN) of the Republic of Indonesia
ERLANA NINDYA MAULIDA Quality and Research Division, the Center of Narcotics Laboratory of National Narcotics Board (BNN) of the Republic of Indonesia

DOI:

https://doi.org/10.22159/ijap.2021v13i5.41910

Keywords:

Kratom, Mitragynine, TLC technique, Validation, The concentration of mitragynine, LC, PDA detector

Abstract

Objective: This study details the determination of mitragynine in various kratom samples using the thin-layer chromatography (TLC) technique and validation of analytical methods for quantifying the concentration of mitragynine in various kratom samples using liquid chromatography with photodiode array detector (LC-PDA).

Methods: TLC technique using n-hexane: ethyl acetate: ammonia 25% (30: 15: 1 v/v/v) was applied to isolate mitragynine from kratom samples. Several interesting spots obtained were visualized under UV light at 254 nm. Samples were also prepared with organic solvent extraction directly prior to LC analysis (non-isolation method of preparation) to quantify the concentration of mitragynine. Mobile phases used were acetonitrile (MP A) and 0.1% formic acid in water (MP B). Samples and standards were run by gradient elution with a flow rate of 0.3 ml/min, detection using PDA detector at 254 nm.

Results: Mitragynine was successfully isolated from kratom samples in R_f 0.50 by TLC system applied. The validation of analytical methods for mitragynine passed the acceptance criteria as described by UNODC Guidance. The concentration of mitragynine in various kratom samples seized in Indonesia ranged from 0.37%-1.70% (%w/w).

Conclusion: Both TLC and LC analytical methods could be applied to determine and quantify the concentration of mitragynine in each examined sample, respectively.

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Author Biography

WIDIANTI NINGTIAS, Quality and Research Division, the Center of Narcotics Laboratory of National Narcotics Board (BNN) of the Republic of Indonesia

Author

References

Wahyono S, Widowati L, Handayani L, Sampurno OD, Haryanti S, Fauzi, et al. Kratom: health and socio-economic prospects. Jakarta: Publisher of National Institute of Health Research and Development; 2019. Available from: http://repository. litbang.kemkes.go.id/id/eprint/3869. [Last accessed on 06 Apr 2021].

Chien GCC, Odonkor C, Amorapanth P. Is kratom the new ‘legal high’ on the block?: the case of an emerging opioid receptor agonist with substance abuse potential. Pain Phys 2017;20: E195-8.

Corkery JM, Streete P, Claridge H, Goodair C, Papanti D, Orsolini L, et al. Characteristics of deaths associated with kratom use. J Psychopharmacol 2019;33:1102-23.

Takayama H. Chemistry and pharmacology of analgesic indole alkaloids from the rubiaceous plant, Mitragyna speciosa. Chem Pharm Bull 2004;52:916-28.

Kikura Hanajiri R, Kawamura M, Maruyama T, Kitajima M, Takayama H, Goda Y. Simultaneous analysis of mitragynine, 7-hydroxymitragynine, and other alkaloids in the psychotropic plant “kratom” (Mitragyna speciosa) by LC-ESI-MS. Forensic Toxicol 2009;27:67-74.

Cinosi E, Martinotti G, Simonato P, Singh D, Demetrovics Z, Roman Urrestarazu A, et al. Following “the roots” of kratom (Mitragyna speciosa): the evolution of an enhancer from a traditional use to increase work and productivity in Southeast Asia to a recreational psychoactive drug in western countries. Biomed Res Int 2015;2015:1-11.

Orio L, Alexandru L, Cravotto G, Mantegna S, Barge A. UAE, MAE, SFE-CO2 and classical methods for the extraction of Mitragyna speciosa leaves. Ultrason Sonochem 2012;19:591-5.

Boffa L, Ghe C, Barge A, Muccioli G, Cravotto G. Alkaloid profiles and activity in different Mitragyna speciosa strains. Nat Prod Commun 2018;13:1111-6.

Chittrakarn S, Penjamras P, Keawpradub N. Quantitative analysis of mitragynine, codeine, caffeine, chlorpheniramine and phenylephrine in a kratom (Mitragyna speciosa Korth.) cocktail using high-performance liquid chromatography. Forensic Sci Int 2012;217:81-6.

Laboratory and Scientific Section, United Nations Office on Drugs and Crime. Guidance for the validation of analytical methodology and calibration of equipment used for testing of illicit drugs in seized materials and biological specimens. New York: United Nations; 2009.

Kowalczuk AP, Lozak A, Zjawiony JK. Comprehensive methodology for identification of kratom in police laboratories. Forensic Sci Int 2013;233:238-43.

Padh H, Parmar S, Patel B. Development and validation of stability-indicating HPTLC method for estimation of swertiamarin in bulk and dosage form. Int J Appl Pharm 2017;9:80-4.

Gurupriya S, Cathrine L, Pratheema P. HPTLC method for the determination of lupeol from Andrographis echioides leaves. Int J Pharm Pharm Sci 2018;10:102-7.

Casey CR, Conley T, Heise A, Thomas T, Ayres PR. Quantitative and qualitative analysis of mitragynine in kratom (Mitragyna speciosa) by GC-MS, LC-MS/MS and UPLC-PDA. J Reg Sci 2015;2:1-14.

Jeyaleela GD, Monisha SI, Vimala JR, Immaculate AA. Isolation of 2-chlorobenzimidazole from Melia dubia leaf extract and its structural characterization. Int J Pharm Pharm Sci 2017;9:67–72.

Chan KB, Pakiam C, Rahim RA. Psychoactive plant abuse: the identification of mitragynine in ketum and in ketum preparations. Bull Narc 2005;57:249-56.

Mcnair H, Polite LN. Troubleshooting in high-performance liquid chromatography. Sep Sci Technol 2007;8:459-77.