IN VIVO ANTIDIABETIC SCREENING OF KABAU (ARCHIDENDRON BUBALINUM (JACK) I. C NIELSEN) SEEDS

HESTI RIASARI; NOVI IRWAN FAUZI; KUSNANDAR ANGGADIREDJA; RIKA HARTATI; SUKRASNO

doi:10.22159/ijap.2021.v13s4.43865

Authors

HESTI RIASARI Department of Pharmaceutical Biology, School of Pharmacy, Institut Teknologi Bandung, Jl Ganesha 10, Bandung 40132, West Java, Indonesia, Indonesian School of Pharmacy, Jl Soekarno Hatta 354, Bandung 40266 West Java, Indonesia
NOVI IRWAN FAUZI Indonesian School of Pharmacy, Jl Soekarno Hatta 354, Bandung 40266 West Java, Indonesia
KUSNANDAR ANGGADIREDJA Department of Pharmaceutical Biology, School of Pharmacy, Institut Teknologi Bandung, Jl Ganesha 10, Bandung 40132, West Java, Indonesia
RIKA HARTATI Department of Pharmaceutical Biology, School of Pharmacy, Institut Teknologi Bandung, Jl Ganesha 10, Bandung 40132, West Java, Indonesia
SUKRASNO Department of Pharmaceutical Biology, School of Pharmacy, Institut Teknologi Bandung, Jl Ganesha 10, Bandung 40132, West Java, Indonesia

DOI:

https://doi.org/10.22159/ijap.2021.v13s4.43865

Keywords:

Antidiabetic, Archidendron bubalinum, MDA, SOD enzyme, Ethanol extract, Fraction active

Abstract

Objective: Study described the screening potential antidiabetic activity of kabau seed extract and fraction.

Methods: The powdered crude drugs weighing 1349.32 grams were extracted with a solvent with solvents with escalating polarity by using soxhletation. The solvents used were n-hexane, ethyl acetate, and 96% ethanol. Screening activity using three variations on doses on the three extracts using the glucose test tolerance method, then the alloxan induction and high-fat feed induction testing methods using selected doses, decreasing blood glucose levels using the GOD PAP enzyme and decreasing MDA levels and increased levels of the enzyme SOD. Extracts that have potential antidiabetic activity are fractionated using liquid-solid fractionation; then the fraction is screened for antidiabetic activity using the glucose test tolerance method.

Results: Screening for antidiabetic activity on the three extracts using the glucose test tolerance method showed that the ethanol extract at a dose of 250 mg/kg BW. The three extracts were then screened for the next mechanism using the alloxan induction method and high-fat feed induction, the decrease in blood sugar levels by the GOD-PAP method showed a good decrease in the ethanol extract by 202.94±2 mg/dl, the three extracts showed good less significant, in the SOD enzyme method, the ethanol extract gave a good value such as the positive control value. Testing on fraction can decrease in blood sugar; the results showed that the ethanol extract and methanol fraction gave a small AUC _0-150 (32695,3 and 33167,71), where the value was close to the result of the glibenclamide 30238,48.

Conclusion: The antidiabetic activity of the extract showed that the ethanol extract was better with the glucose test tolerance method, with alloxan induction animal models and high-fat feed induction. In the methanol fraction derived from 96% ethanol extract, it provides a good reduction in blood sugar levels in the screening method with a glucose test tolerance

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