CHARACTERIZATION OF NANOPARTICLES CONDITIONED MEDIUM ADIPOSE TISSUE MESENCHYMAL STEM CELL (CM-ATMSC)

Authors

  • DENI RAHMAT Faculty of Pharmacy, Pancasila University, Jl. Srengseng Sawah, Jagakarsa, South Jakarta 12640, Indonesia
  • WAHYU WIDOWATI Maranatha Christian University, Jl. Surya Sumantri No 65, Bandung 40164, West Java, Indonesia
  • AHMAD FARIED Faculty of Medicine, Padjadjaran University, JL. Raya Bandung Sumedang, Jatinangor, West Java, Indonesia
  • ITA MARGARETHA NAINGGOLAN Atma Jaya Catholic University, Jl. Jenderal Sudirman No.51, South Jakarta 12930, Indonesia
  • DIDIK PRIYANDOKO Faculty of Mathematic and Natural Science Education, Indonesia University of Education, Jl. Dr. Setiabudi No. 229, Bandung 40154, West Java, Indonesia
  • ANARISA BUDIATI Faculty of Pharmacy, Pancasila University, Jl. Srengseng Sawah, Jagakarsa, South Jakarta 12640, Indonesia
  • TEDDY MARCUS ZAKARIA Maranatha Christian University, Jl. Surya Sumantri No 65, Bandung 40164, West Java, Indonesia
  • ERVI AFIFAH PT Aretha Medika Utama, Jl. Babakan Jeruk II No. 9, Bandung 40163, West Java, Indonesia

DOI:

https://doi.org/10.22159/ijap.2022.v14s3.22

Keywords:

Nanoparticles, Drug delivery, Conditioned medium, Stem cells

Abstract

Objective: The aims of this study were to formulate and characterize the nanoparticles containing conditioned medium adipose tissue

mesenchymal stem cell (CM-ATMSC).

Methods: Adipose Tissue Mesenchymal Stem Cell (AT-MSC) was cultured with supplemented Modified Eagle Medium (MEM) Alpha in an incubator with 37 °C and 5% CO2. The conditioned medium was collected when the cells were confluent. Nanoparticles were characterized in the term of morphology using transmission electron microscopy (TEM), particle size, zeta potential, loading capacity, entrapment efficiency, and drug release.

Results: CM-ATMSC nanoparticles had the smallest particle size, namely chitosan-inulin nanoparticles with particle size of 128 nm, and the largest particle was modified chitosan (thiomer)-fucoidan nanoparticles with particle size of 254.3 nm. Based on zeta potential results, it is known that the resulting nanoparticle suspension was stable.

Conclusion: The resulting nanoparticle suspension was stable and the smallest CM-ATMSC nanoparticle demonstrated the particle size of 128 nm. The results showed that the nanoparticles of CM-ATMSC have been successfully prepared.

Downloads

Download data is not yet available.

References

Widowati W, Wijaya L, Bachtiar I, Gunanegara RF, Sugeng SU, Irawan YA. Effect of oxygen tension on proliferation and characteristics of Wharton’s jelly derived mesenchymal stem cells. Biomark Genom Med. 2014;6(1):43-8. doi: 10.1016/ j.bgm.2014.02.001.

Marlina M, Rahmadian R, Armenia A, Widowati W, Rizal R, Kusuma HSW, Wibowo SHB, Widodo WS, Sholihah IA. Isolation, characterization, proliferation and differentiation of synovial membrane-derived mesenchymal stem cells (SM-MSCs) from osteoarthritis patients. MCBS 2020;4:76-82.

Su Y, Zhang T, Huang T, Gao J. Current advances and challenges of mesenchymal stem cells-based drug delivery system and their improvements. Int J Pharm. 2021;600:120477. doi: 10.1016/j.ijpharm.2021.120477, PMID 33737099.

Suryaprakash S, Lao YH, Cho HY, Li M, Ji HY, Shao D. Engineered mesenchymal stem cell/nanomedicine spheroid as an active drug delivery platform for combinational glioblastoma therapy. Nano Lett. 2019;19(3):1701-5. doi: 10.1021/acs.nanolett.8b04697, PMID 30773888.

Qi Y, Yang Z, Ding Q, Zhao T, Huang Z, Feng G. Targeted transplantation of iron oxide-labeled, adipose-derived mesenchymal stem cells in promoting meniscus regeneration following a rabbit massive meniscal defect. Exp Ther Med. 2016;11(2):458-66. doi: 10.3892/etm.2015.2944, PMID 26893631.

Zhang T, Li F, Xu Q, Wang Q, Jiang X, Liang Z. Ferrimagnetic nanochains-based mesenchymal stem cell engineering for highly efficient post-stroke recovery. Adv Funct Mater. 2019;29(24):1-13. doi: 10.1002/adfm.201900603.

Rahmat D, Nurhidayati L, Bathini MA, Ekstrak Nanas PAA (Ananas Comosus (L.). Merr) dengan pembentukan nanopartikel. J Sains Kesehat; 2016. p. 236-44.

Aouache R, Biquard L, Vaiman D, Miralles F. Oxidative stress in preeclampsia and placental diseases. Int J Mol Sci. 2018;19(5):1496. doi: 10.3390/ijms19051496, PMID 29772777.

Lister INE, Ginting CN, Girsang E, Amansyah A, Chiuman L, Yanti N, Rizal R. Hepatoprotective effect of eugenol on acetaminophen-induced hepatotoxicity in Hep G2 cells. J Phys Conf Ser. 2019;1374:1-7.

Luo R, Wang Y, Xu P, Cao G, Zhao Y, Shao X. Hypoxia-inducible miR-210 contributes to preeclampsia via targeting thrombospondin type I domain-containing. Sci Rep 2016;7A:1-11.

Rahmat D, Farida Y, Brylianto AT, Sumarny R, Kumala S. Antidiabetic activity of nanoparticles containing Javanese turmeric rhizome extract: the strategy to change particle size. Int J App Pharm. 2020;12:90-3. doi: 10.22159/jap.2020v12i4.36249.

Syarmalina WD, Rahmat D. Nanoparticles formulation of temulawak extract based on chitosan as antiacne. Med Sains. 2019;3:153-8.

Published

28-06-2022

How to Cite

RAHMAT, D., WIDOWATI, W., FARIED, A., NAINGGOLAN, I. M., PRIYANDOKO, D., BUDIATI, A., ZAKARIA, T. M., & AFIFAH, E. (2022). CHARACTERIZATION OF NANOPARTICLES CONDITIONED MEDIUM ADIPOSE TISSUE MESENCHYMAL STEM CELL (CM-ATMSC). International Journal of Applied Pharmaceutics, 14(3), 104–106. https://doi.org/10.22159/ijap.2022.v14s3.22

Issue

Section

Original Article(s)

Most read articles by the same author(s)

<< < 1 2