A MODEST UV SPECTROPHOTOMETRIC ASSISTED BY CHEMOMETRIC APPROACH FOR VERIFICATION OF ACETAMINOPHEN LEVEL IN VARIOUS MANUFACTURED TABLETS AND SYRUPS IN INDONESIAN PHARMACIES

MUHAMMAD ABDURRAHMAN MUNIR; AHLAM INAYATULLAH; SOFIAN IBRAHIM; IMRAM RADNE RIMBA PUTRI; EMELDA EMELDA; ANNISA FATMAWATI; NURHIDAYANTI NURHIDAYANTI

doi:10.22159/ijap.2023v15i1.46290

Authors

MUHAMMAD ABDURRAHMAN MUNIR Department of Pharmacy, Faculty of Health Sciences, Alma Ata University, Bantul, Yogyakarta, 55184, Indonesia https://orcid.org/0000-0001-6129-6202
AHLAM INAYATULLAH Department of Food Technology, Faculty of Chemical Engineering, Politeknik Sriwijaya, Palembang, 30139, Indonesia
SOFIAN IBRAHIM Malaysian Nuclear Agency, 43000 Kajang, Bangi, Malaysia
IMRAM RADNE RIMBA PUTRI Department of Hospital Administration, Faculty of Health Sciences, Alma Ata University, Bantul, Yogyakarta, 55184, Indonesia
EMELDA EMELDA Department of Pharmacy, Faculty of Health Sciences, Alma Ata University, Bantul, Yogyakarta, 55184, Indonesia
ANNISA FATMAWATI Department of Pharmacy, Faculty of Health Sciences, Alma Ata University, Bantul, Yogyakarta, 55184, Indonesia
NURHIDAYANTI NURHIDAYANTI Department of Pharmacy, Faculty of Health Sciences, Alma Ata University, Bantul, Yogyakarta, 55184, Indonesia

DOI:

https://doi.org/10.22159/ijap.2023v15i1.46290

Keywords:

Acetaminophen, Pharmacies, Spectrophotometer, Syrups, Tablets

Abstract

Objective: This study aimed to verify the paracetamol level in some fabricated tablets and syrups in Indonesian pharmacies.

Methods: The fabricated tablets and syrups were analyzed using a spectrophotometer UV that was assisted by the chemometric approach. Partial least squares (PLS) and principal component regression (PCR) were the chemometric methods employed to verify the paracetamol level in pharmaceutical products. There were 25 different samples (tablets and syrups) applied in this study. The validation study was employed in this study to verify the approach according to the ICH guidelines. The double-distilled water was applied as a solvent before the samples were analyzed using a spectrophotometer.

Results: This technique was efficient and require double-distilled water only as a solvent. The results of this study reveal that there was a deviation in absorbance of the samples with RSD ranging from (0.15-0.45). The technique was linear, ranging from 1.0–6.0 µg·ml^-1, with an R² (0.9991) obtained at 242 nm. The percentage recovery was applied to study the accuracy of the technique and was acquired at 99.18%. The results have shown that the approach was the potential to be applied in estimating the level of paracetamol in tablets and syrups.

Conclusion: The detection of paracetamol levels in tablets and syrups using UV spectrophotometric showed satisfactory outcomes. The application of the chemometric approach by using PLC and PCR as the statistical assessment indicated that there was no significant distinction among the validated methods. Furthermore, the method can be used by industries particularly small industries to secure medicines that comply with Indonesian rules.

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