EXTRACTION AND CHARACTERIZATION OF BLACK BETEL LEAF (PIPER ACRE BLUME.) ESSENTIAL OILS FROM EAST KALIMANTAN

VINA MAULIDYA; ALIYA NUR HASANAH; LAODE RIJAI; MUCHTARIDI MUCHTARIDI

doi:10.22159/ijap.2022.v14s4.OP02

Authors

VINA MAULIDYA Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy Universitas Padjajaran Jl. Raya Jatinangor KM 21.5 Bandung, Sumedang, 45363, Faculty of Pharmacy Universitas Mulawarman, Samarinda, Indonesia
ALIYA NUR HASANAH Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy Universitas Padjajaran Jl. Raya Jatinangor KM 21.5 Bandung, Sumedang, 45363
LAODE RIJAI Faculty of Pharmacy Universitas Mulawarman, Samarinda, Indonesia
MUCHTARIDI MUCHTARIDI Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy Universitas Padjajaran Jl. Raya Jatinangor KM 21.5 Bandung, Sumedang, 45363

DOI:

https://doi.org/10.22159/ijap.2022.v14s4.OP02

Keywords:

Extraction, Characteristics, Essential oil, Black betel

Abstract

Objective: To determine the extraction technique and content of linalool, as the dominant compound, in black betel essential oil from East Kalimantan using a validated analytical method.

Methods: Extraction was carried out using steam distillation. Essential oils were assessed by observing the colour and solubility in ethanol. Using an Agilent GC-MS with a 5977B (GCMSD) detector, a DB-5MS column, and a helium gas carrier of 1 ml/minute. The level of dominant compounds was determined using a validated analytical method.

Results: The essential oil had characteristics that met SNI standards. Based on the results of GC-MS, the dominant compound in five samples was linalool. Validation of the analytical method was carried out with the following conditions established: injector temperature 250 °C, oven temperature 40 °C with a 2 min holding time, reaching 125 °C with a 10 min holding time, reaching 250 °C with a 2 min holding time, reaching
340 °C with a 10 min holding time. The resulted in a linearity of 0.999; a 1.637% relative standard deviation for precision; 102.27% recovery value for accuracy; 0.4% LOD; and 1.2% LOQ. The percentage linalool content of the samples was found in the sample code MADSH 4 (10.56% with a standard deviation of 0.6169).

Conclusion: The steam distillation extraction method can be used to obtain essential oil with characteristics that meet SNI standards. Based on the results of GC-MS, linalool is the dominant compound, with a concentration of 10.56% found in the MADSH4 sample.

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