DEVELOPMENT, CHARACTERIZATION AND ANTIBACTERIAL PROPERTIES OF SILVER NANOPARTICLES LOADED SODIUM ALGINATE/XANTHAN GUM MICROBEADS FOR DRUG DELIVERY APPLICATIONS

E. VENKATA RAMANA; NASEEM

doi:10.22159/ijap.2023v15i3.47028

Authors

E. VENKATA RAMANA Department of Pharmaceutical Chemistry, Telangana University, Nizamabad, Telangana 503322, India
NASEEM Department of Pharmaceutical Chemistry, Telangana University, Nizamabad, Telangana 503322, India https://orcid.org/0009-0002-8741-5083

DOI:

https://doi.org/10.22159/ijap.2023v15i3.47028

Keywords:

Sodium alginate, Xanthan gum, Silver nanoparticles, Microbeads, Ofloxacin, Antibacterial activity

Abstract

Objective: The aim of this study is to create pH-responsive drug carriers, which are useful because they have the potential to improve treatment efficacy by controlling the release rate of ofloxacin from the polymer matrix.

Methods: In the first step, silver nanoparticles (Ag NPs) were synthesized from silver nitrate using leaf extract of phyllanthus urinaria L as a reducing agent. In the second step, Ag-NPs-loaded polymeric microbeads were synthesized using sodium alginate (SA) and xanthan gum (XG) for controlled release of ofloxacin (OFLX). The developed microbeads were characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (X-RD), transition electron microscopy (TEM), Selected Area Electron Diffraction (SAED), and scanning electron microscopy (SEM). Swelling and in vitro release studies were performed at pH 2.0 and 7.4 at 37 °C. The in vitro antibacterial activity of microbeads were tested against S. mutans, K. pneumoniae, and B. subtilis. The release kinetics and mechanism were analyzed by fitting the release data into different kinetic models and the korsmeyer-peppas equation.

Results: FTIR confirms the generation of silver nanoparticle and also the generation of polymeric microbeads. SEM studies reveal the developed microbeads are spherical in shape with rough surfaces. TEM studies reveal the size of 20-40 nm. XRD analysis reveals the molecular dispersion of DOX and the presence of silver nanoparticles in the polymeric matrix. Investigations of in vitro release and swelling studies show that the developed microbeads are relatively suitable for intestinal drug delivery because higher release rate was observed at pH 7.4. The developed microbead follows non-Fickian diffusion drug release mechanism. The created samples exhibited antimicrobial activity against S. mutans, K. pneumoniae, and B. subtilis.

Conclusion: The results indicate that microbeads containing OFLX and silver nanoparticles are effective drug-delivery vehicles. A further warrant is required for the use of manufactured microbeads in drug delivery applications.

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References

Krithiga N, Rajalakshmi A, Jayachitra A. Green synthesis of silver nanoparticles using leaf extracts of clitoria ternatea and solanum nigrum and study of its antibacterial effect against common nosocomial pathogens. J Nanosci. 2015;2015:1-8. doi: 10.1155/2015/928204.

Bayda S, Adeel M, Tuccinardi T, Cordani M, Rizzolio F. The history of nanoscience and nanotechnology: from chemical–physical applications to nanomedicine. Molecules. 2019;25(1):112. doi: 10.3390/molecules25010112, PMID 31892180.

Soares S, Sousa J, Pais A, Vitorino C. Nanomedicine: principles, properties, and regulatory issues. Front Chem. 2018;6:360. doi: 10.3389/fchem.2018.00360, PMID 30177965.

Patra JK, Das G, Fraceto LF, Campos EVR, Rodriguez Torres MDP, Acosta Torres LS. Nano-based drug delivery systems: recent developments and future prospects. J Nanobiotechnology. 2018;16(1):71. doi: 10.1186/s12951-018-0392-8, PMID 30231877.

Kalpana VN, Devi Rajeswari V. A review on green synthesis, biomedical applications, and toxicity studies of ZnO NPs. Bioinorg Chem Appl. 2018;2018:3569758. doi: 10.1155/2018/3569758, PMID 30154832.

Ghosh S, Ahmad R, Zeyaullah M, Khare SK. Microbial nano-factories: synthesis and biomedical applications. Front Chem. 2021;9:626834. doi: 10.3389/fchem.2021.626834, PMID 33937188.

Singhal G, Bhavesh R, Kasariya K, Sharma AR, Singh RP. Biosynthesis of silver nanoparticles using ocimum sanctum (Tulsi) leaf extract and screening its antimicrobial activity. J Nanopart Res. 2011;13(7):2981-8. doi: 10.1007/s11051-010-0193-y.

Varghese Alex K, Tamil Pavai P, Rugmini R, Shiva Prasad M, Kamakshi K, Sekhar KC. Green synthesized ag nanoparticles for bio-sensing and photocatalytic applications. ACS Omega. 2020;5(22):13123-9. doi: 10.1021/acsomega.0c01136, PMID 32548498.

Ibrahim N, Jamaluddin ND, Tan LL, Mohd Yusof NY. A review on the development of gold and silver nanoparticles-based biosensor as a detection strategy of emerging and pathogenic RNA virus. Sensors (Basel). 2021;21(15):5114. doi: 10.3390/s21155114, PMID 34372350.

Loiseau A, Asila V, Boitel Aullen G, Lam M, Salmain M, Boujday S. Silver-based plasmonic nanoparticles for and their use in biosensing. Biosensors. 2019;9(2):78. doi: 10.3390/bios9020078, PMID 31185689.

Zhang XF, Liu ZG, Shen W, Gurunathan S. Silver nanoparticles: synthesis, characterization, properties, applications, and therapeutic approaches. Int J Mol Sci. 2016;17(9):1534. doi: 10.3390/ijms17091534, PMID 27649147.

Bruna T, Maldonado Bravo F, Jara P, Caro N. Silver nanoparticles and their antibacterial applications. Int J Mol Sci. 2021;22(13):7202. doi: 10.3390/ijms22137202, PMID 34281254.

Marambio Jones C, Hoek EMV. A review of the antibacterial effects of silver nanomaterials and potential implications for human health and the environment. J Nanopart Res. 2010;12(5):1531-51. doi: 10.1007/s11051-010-9900-y.

Farooqui MA, Chauhan PS, Krishnamoorthy P, Shaik J. Extraction of silver nanoparticles from the leaf extracts of clerodendrum inerme. Dig J Nanomater Biostructures. 2010;5(1):43-9.

Paladini F, Pollini M. Antimicrobial silver nanoparticles for wound healing application: progress and future trends. Materials (Basel). 2019;12(16):2540. doi: 10.3390/ma12162540, PMID 31404974.

Yin IX, Zhang J, Zhao IS, Mei ML, Li Q, Chu CH. The antibacterial mechanism of silver nanoparticles and its application in dentistry. Int J Nanomedicine. 2020;15:2555-62. doi: 10.2147/IJN.S246764, PMID 32368040.

Liechty WB, Kryscio DR, Slaughter BV, Peppas NA. Polymers for drug delivery systems. Annu Rev Chem Biomol Eng. 2010;1:149-73. doi: 10.1146/annurev-chembioeng-073009-100847, PMID 22432577.

Borandeh S, Van Bochove B, Teotia A, Seppala J. Polymeric drug delivery systems by additive manufacturing. Adv Drug Deliv Rev. 2021;173:349-73. doi: 10.1016/j.addr.2021.03.022, PMID 33831477.

Obireddy SR, Chintha M, Kashayi CR, Venkata KRKS, Subbarao SMC. Gelatin-coated dual cross-linked sodium alginate/magnetite nanoparticle microbeads for controlled release of doxorubicin. Chemistry Select. 2020;5(33):10276-84. doi: 10.1002/slct.202002604.

Sreekanth Reddy O, Subha MCS, Jithendra T, Madhavi C, Chowdoji Rao K. Curcumin encapsulated dual cross-linked sodium alginate/montmorillonite polymeric composite beads for controlled drug delivery. J Pharm Anal. 2021;11(2):191-9. doi: 10.1016/j.jpha.2020.07.002. PMID 34012695.

Reddy OS, Subha MCS, Jithendra T, Madhavi C, Rao KC. Fabrication and characterization of smart karaya gum/sodium alginate semi-IPN microbeads for controlled release of d-penicillamine drug. Polym Polym Compos. 2021;29(3):163-75. doi: 10.1177/0967391120904477.

Lanjhiyana SK, Bajpayee P, Kesavan K, Lanjhiyana S, Muthu MS. Chitosan–sodium alginate blended polyelectrolyte complexes as potential multiparticulate carrier system: colon-targeted delivery and gamma scintigraphic imaging. Expert Opin Drug Deliv. 2013;10(1):5-15. doi: 10.1517/17425247.2013.734805, PMID 23106236.

Biswas A, Mondal S, Das SK, Bose A, Thomas S, Ghosal K. Development and characterization of natural product derived macromolecules based interpenetrating polymer network for therapeutic drug targeting. ACS Omega. 2021;6(43):28699-709. doi: 10.1021/acsomega.1c03363, PMID 34746564.

Reddy OS, Subha MCS, Jithendra T, Madhavi C, Rao KC, Mallikarjuna B. Sodium alginate/gelatin microbeads-intercalated with kaolin nanoclay for emerging drug delivery in Wilson’s disease. Int J App Pharm. 2019;11:71-80. doi: 10.22159/ijap.2019v11i5.34254.

Kuppuswami GM. Fermentation (Industrial), production of xanthan gum. In: Batt CA, Tortorello ML, editors. Encyclopedia of food microbiology. 2nd ed. Cambridge: Academic Press; 2014. p. 816-21.

Jithendra T, Reddy OS, Subha MCS, Madhavi C, Rao KC. Xanthan gum graft copolymer/sodium alginate microbeads coated with chitosan for controlled release of chlorthalidone drug. Int J Pharm Sci Res. 2020;11(3):1132-45.

Kar R, Mohapatra S, Bhanja S, Das D, Barik B. Formulation and in vitro characterization of xanthan gum-based sustained release matrix tables of isosorbide-5-mononitrate. Iran J Pharm Res. 2010;9(1):13-9. PMID 24363701.

Tao Y, Zhang R, Xu W, Bai Z, Zhou Y, Zhao S. Rheological behavior and microstructure of release-controlled hydrogels based on xanthan gum crosslinked with sodium trimetaphosphate. Food Hydrocoll. 2016;52:923-33. doi: 10.1016/j.foodhyd.2015.09.006.

Marslin G, Revina AM, Khandelwal VKM, Balakumar K, Sheeba CJ, Franklin G. Pegylated ofloxacin nanoparticles render strong antibacterial activity against many clinically important human pathogens. Colloids Surf B Biointerfaces. 2015;132:62-70. doi: 10.1016/j.colsurfb.2015.04.050, PMID 26005932.

Pallerla D, Banoth S, Jyothi S. Fabrication of nano clay intercalated polymeric microbeads for controlled release of curcumin. Int J App Pharm. 2021;13(1):206-15. doi: 10.22159/ijap.2021v13i1.39965.

Chintha M, Obireddy SR, Areti P, Marata Chinna Subbarao S, Kashayi CR, Rapoli JK. Sodium alginate/locust bean gum-g-methacrylic acid IPN hydrogels for “simvastatin” drug delivery. J Dispers Sci Technol. 2020;41(14):2192-202. doi: 10.1080/01932691.2019.1677247.

Costa P, Sousa Lobo JM. Modeling and comparison of dissolution profiles. Eur J Pharm Sci. 2001;13(2):123-33. doi: 10.1016/S0928-0987(01)00095-1, PMID 11297896.

Obireddy SR, Lai WF. Preparation and characterization of 2-hydroxyethyl starch microparticles for co-delivery of multiple bioactive agents. Drug Deliv. 2021;28(1):1562-8. doi: 10.1080/10717544.2021.1955043, PMID 34286634.

Jakinala P, Lingampally N, Hameeda B, Sayyed RZ, Khan MY, Elsayed EA. Silver nanoparticles from insect wing extract: biosynthesis and evaluation for antioxidant and antimicrobial potential. PLOS ONE. 2021;16(3):e0241729. doi: 10.1371/journal.pone.0241729. PMID 33735177.

Cai Y, Piao X, Gao W, Zhang Z, Nie E, Sun Z. Large-scale and facile synthesis of silver nanoparticles via a microwave method for a conductive pen. RSC Adv. 2017;7(54):34041-8. doi: 10.1039/C7RA05125E.