THE EFFECT OF ADDITION 5% PROPOLIS NANOPARTICLES IN EPOXY RESIN AND BIOCERAMIC SEALERS ON THE GROWTH OF ENTEROCOCCUS FAECALIS ATCC 29212 AND THE DENTINAL TUBULAR PENETRATION: IN VITRO

NELMI WAHYUNI; NEVI YANTI; TRIMURNI ABIDIN; WIDI PRASETIA; DWI SURYANTO

doi:10.22159/ijap.2023v15i4.47486

Authors

NELMI WAHYUNI Department of Conservative Dentistry, Faculty of Dentistry, Universitas Sumatera Utara, Medan, Indonesia https://orcid.org/0009-0002-5836-6008
NEVI YANTI Department of Conservative Dentistry, Faculty of Dentistry, Universitas Sumatera Utara, Medan, Indonesia https://orcid.org/0000-0003-1310-0142
TRIMURNI ABIDIN Department of Conservative Dentistry, Faculty of Dentistry, Universitas Sumatera Utara, Medan, Indonesia
WIDI PRASETIA Department of Conservative Dentistry, Faculty of Dentistry, Universitas Sumatera Utara, Medan, Indonesia
DWI SURYANTO Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Medan, Indonesia

DOI:

https://doi.org/10.22159/ijap.2023v15i4.47486

Keywords:

Propolis nanoparticles, Sealer, Bioceramic, Epoxy resin, E. faecalis, Dentinal tubular penetration

Abstract

Objective: The goal of endodontic treatment is to eliminate bacteria and their products from the root canal. Bacteria are the main etiological factors of pulpal and periapical diseases. Chemomechanical preparation and administration of root canal medicaments cannot completely eliminate bacteria in the root canal system, especially Enterococcus faecalis, which have high resistancy, therefore, an ideal obturation material is required. Adequate root canal filling quality affects the success of endodontic treatment. The aim of this study was to observe the effect of addition 5% propolis nanoparticles to the commercial epoxy resin and the bioceramic sealer on the growth of E. faecalis ATCC 29212 and the dentinal tubular penetration.

Methods: Thirty-five tooth samples were randomly divided into 5 groups. Root canal preparation was carried out with the same working length of 14 mm by cutting the tooth at the Cementoenamel Junction (CEJ); sample was inoculated with E. faecalis ATCC 29212 for 48 h. Obturation used four types of sealer, Group I (epoxy resin with 5% propolis nanoparticles), Group II (bioceramic with 5% propolis nanoparticles), Group III (bioceramic) and Group IV (epoxy resin). Data were analyzed using the Kruskal wallis test with a significant level of p<0.05.

Results: In the bacterial growth test, there was a significant difference in the number of bacterial colonies between the epoxy resin groups with 5% propolis nanoparticles, bioceramic with 5% of propolis nanoparticles, bioceramic and epoxy resin (p = 0.000<0.05). In the dentinal tubular penetration there was a significant difference between epoxy resin with 5% of propolis nanoparticles, bioceramic sealer with 5% propolis nanoparticles, bioceramic, and epoxy resin (p = 0.001<0.05).

Conclusion: The addition of 5% propolis nanoparticles to commercial bioceramic and epoxy resin sealers can eliminate E. faecalis bacteria in the root canals and increase dentinal tubular penetration. Bioceramic sealer has a higher antibacterial effect and dentinal tubular penetration compared to epoxy resin.

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References

Ruddle C. Endodontic disinfection: tsunami irrigation. Saudi Endod J. 2015;5(1):1-12. doi: 10.4103/1658-5984.149080.

Chen H, Zhao X, Qiu Y, Xu D, Cui L, Wu B. The tubular penetration depth and adaption of four sealers: a scanning electron microscopic study. BioMed Res Int. 2017;2017:2946524. doi: 10.1155/2017/2946524, PMID 29479539.

Marissa C, Usman M, Suprastiwi E, Erdiani A, Meidyawati R. Comparison of dentinal tubular penetration of three bioceramic sealers. Int J App Pharm. 2020;12 Special Issue 2:23-6. doi: 10.22159/ijap.2020.v12s2.OP-05.

Kikly A, Jaafoura S, Kammoun D, Sahtout S. Sealing ability of endodontic cements: an in vitro study. Int J Dent. 2020;2020:5862598. doi: 10.1155/2020/5862598, PMID 32104179.

Zavattini A, Knight A, Foschi F, Mannocci F. Outcome of root canal treatments using a new calcium silicate root canal sealer: a non-randomized clinical trial. J Clin Med. 2020;9(3). doi: 10.3390/jcm9030782, PMID 32183124.

Guerreiro Tanomaru JM, Chavez Andrade GM, de Faria Junior NB, Watanabe E, Tanomaru Filho M. Effect of passive ultrasonic irrigation on Enterococcus faecalis from root canals: an ex vivo study. Braz Dent J. 2015;26(4):342-6. doi: 10.1590/0103-6440201300022, PMID 26312969.

Gomes BPFA, Vianna ME, Zaia AA, Almeida JFA, Souza Filho FJ, Ferraz CCR. Chlorhexidine in endodontics. Braz Dent J. 2013;24(2):89-102. doi: 10.1590/0103-6440201302188, PMID 23780357.

Borzini L, Condo R, De Dominicis P, Casaglia A, Cerroni L. Root canal irrigation: chemical agents and plant extracts against Enterococcus faecalis. Open Dent J. 2016;10(1):692-703. doi: 10.2174/1874210601610010692, PMID 28217184.

Daffalla K, Mahmoud A. Propolis as a natural remedy. J Int Oral Heal. 2016;8(5):646-9.

Abdel Raheem IA, Abdul Razek A, Elgendy AA, Labah DA, Saleh NM. Egyptian propolis-loaded nanoparticles as a root canal nanosealer: sealing ability and in vivo biocompatibility. Int J Nanomedicine. 2020;15:5265-77. doi: 10.2147/IJN.S258888, PMID 32884255.

Roelianto M, Wahjuningrum DA, Pamungkas YB, Zubaidah N, Permatasari RI, Dianti E. In vivo study of sealing capability of raw propolis extract and calcium hydroxide on dentin surface. Clin Cosmet Investig Dent. 2020;12:335-41. doi: 10.2147/CCIDE.S243714, PMID 32884357.

Abdel Raheem IA, Abdul Razek A, Elgendy AA, Saleh NM, Shaaban MI, Abd El-Hady FK. Design, evaluation and antimicrobial activity of Egyptian propolis-loaded nanoparticles: intrinsic role as a novel and naturally based root canal nanosealer. Int J Nanomedicine. 2019;14:8379-98. doi: 10.2147/IJN.S219577, PMID 31695372.

Marica A, Fritea L, Banica F, Sinescu C, Iovan C, Hulka I. Carbon nanotubes for improved performances of endodontic sealer. Materials (Basel). 2021;14(15). doi: 10.3390/ma14154248, PMID 34361440.

Kranz S, Guellmar A, Braeutigam F, Tonndorf Martini S, Heyder M, Reise M. Antibacterial effect of endodontic disinfections on enterococcus faecalis in dental root canals-an in vitro model study. Materials (Basel). 2021;14(9). doi: 10.3390/ma14092427, PMID 34066928.

Parolia A, Kumar H, Ramamurthy S, Davamani F, Pau A. Effectiveness of chitosan-propolis nanoparticle against Enterococcus faecalis biofilms in the root canal. BMC Oral Health. 2020;20(1):339. doi: 10.1186/s12903-020-01330-0, PMID 33238961.

Castaldo S, Capasso F. Propolis, an old remedy used in modern medicine. Fitoterapia. 2002;73Suppl 1:S1-6. doi: 10.1016/s0367-326x(02)00185-5, PMID 12495704.

Takaisi Kikuni NB, Schilcher H. Electron microscopic and microcalorimetric investigations of the possible mechanism of the antibacterial action of a defined propolis provenance. Planta Med. 1994 Jun;60(3):222-7. doi: 10.1055/s-2006-959463, PMID 8073087.

Zheng YZ, Zhou Y, Liang Q, Chen DF, Guo R, Lai RC. Hydrogen-bonding interactions between apigenin and ethanol/water: a theoretical study. Sci Rep. 2016 Oct;6:34647. doi: 10.1038/srep34647, PMID 27698481.

Wang Y, Liu S, Dong Y. In vitro study of dentinal tubule penetration and filling quality of bioceramic sealer. PLOS ONE. 2018;13(2):e0192248. doi: 10.1371/journal.pone.0192248, PMID 29390037.

Al-Haddad A, Abu Kasim NH, Che Ab Aziz ZA. Interfacial adaptation and thickness of bioceramic-based root canal sealers. Dent Mater J. 2015;34(4):516-21. doi: 10.4012/dmj.2015-049, PMID 26235718.

El Hachem R, Khalil I, Le Brun G, Pellen F, Le Jeune B, Daou M. Dentinal tubule penetration of AH Plus, BC Sealer and a novel tricalcium silicate sealer: a confocal laser scanning microscopy study. Clin Oral Investig. 2019;23(4):1871-6. doi: 10.1007/s00784-018-2632-6, PMID 30225679.