FORMULATION AND EVALUATION OF DISPERSIBLE TABLETS OF FLAVONOID PGAL ISOLATED FROM SARACA ASOCA LEAVES

SONIKA SHRIVASTAV; KAMAL SINGH RATHORE; NEELKANT PRASAD

doi:10.22159/ijap.2023v15i4.47855

Authors

SONIKA SHRIVASTAV B. N. College of Pharmacy, B. N. University, Udaipur, Rajasthan, India https://orcid.org/0000-0003-2791-1624
KAMAL SINGH RATHORE B. N. College of Pharmacy, B. N. University, Udaipur, Rajasthan, India https://orcid.org/0000-0002-7980-1059
NEELKANT PRASAD SGT College of Pharmacy, SGT University, Budhera, Gurugram, Haryana, India https://orcid.org/0000-0002-8333-8810

DOI:

https://doi.org/10.22159/ijap.2023v15i4.47855

Keywords:

Antidepressant activity, Dispersible tablets, Methanolic extract, Saraca asoca leaves, Phytoconstituent

Abstract

Objective: The study aimed to design and evaluate a dispersible tablet of flavonoid PGAL isolated from Saraca asoca leaves for antidepressant activity.

Methods: The phytoconstituent was isolated from a methanolic extract of Saraca asoca leaves using silica gel column (60-120 mesh) chromatography. The dispersible tablets were prepared by direct compression and then evaluated for various tablet evaluation parameters and antidepressant activity, performing Tail Suspension Test (TST), Forced Swim Test (FST), Locomotion activity, Brain glutamate level and brain nitrite level.

Results: Hardness of 2.85±0.13 kg/cm² to 3.25±0.15 kg/cm² and friability of 0.35% to 0.48% indicate that the prepared tablets were mechanically sound. Test for weight variation was also within tolerance limits, i.e. 2.04% to 4.25% difference in weight of the tablet from the average weight of 10 tablets. The tablets also passed the test for drug content uniformity, 97.35% to 100.35%, i.e. always within the prescribed limits of 95% to 105%. Disintegration time, 2 min to 2.75 min, and dispersion time, 3.25 min to 3.75 min, were also exemplary. The antidepressant activity was displayed by the optimized formulation as indicated by a significant decrease (p<0.05) in immobility time in TST as well as FST; a significant decrease (p<0.05) in the level of brain tissue glutamate as well as nitrite in PGAL formulation treated mice when compared with negative control, as did by standard drug fluoxetine.

Conclusion: The formulation has been optimized based on dispersion time. The formulation with minimum dispersion time, i.e. F1, has been considered an optimized formulation. The prepared optimized formulation was found to comply with all physical parameters and antidepressant activity.

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References

Mathew S, Mathew G, Joy PP, Skari BP, Joseph TS. Differentiation of Saraca asoca crude drug from its adulterant. Anc Sci Life. 2005 Apr;24(4):174-8. PMID 22557174.

Smitha GR, Thondaiman V. Reproductive biology and breeding system of Saraca asoca (Roxb.) De wilde: a vulnerable medicinal plant. Springerplus. 2016 Nov 28;5(1):2025. doi: 10.1186/s40064-016-3709-9, PMID 27995002.

Jaganathan R, Parasuram M, Ravindran R, Vijayaragavan D. Phytochemical screening and evaluation of antimicrobial activity of Saraca asoca flower extract. Int J Pharm Technol. 2017 Apr;9(1):28257-71.

Lin LJ, Huang XB, Lv ZC. Isolation and identification of flavonoids components from pteris vittata L. Springerplus. 2016 Sep 23;5(1):1649. doi: 10.1186/s40064-016-3308-9, PMID 27722067.

Granero GE, Ramachandran C, Amidon GL. Gastrointestinal dissolution and absorption of drugs. In: Waterbeemed HVD, Lennemas H, Artursson P. editors Mannhold R, Kubinyi H, Folkers G. Series editors. Drug bioavailability, methods and principles in medicinal chemistry. 2nd ed. Weinheim: Wiley-VCH Press; 2003. p. 189-214. doi: 10.1002/3527601473.ch8.

Augsburger LL, Zellhofer MJ. Tablet formulation. In: Swarbrick J. editor. Encyclopedia of pharmaceutical technology. 3rd ed. Vol. 6. New York: Informa Healthcare; 2007. p. 3641-52.

Perrie Y, Rades T. Themed issue: improve dissolution, solubility and bioavailability of poorly soluble drugs. J Pharm Pharmacol. 2010 Nov;62(11):1517-8. doi: 10.1111/j.2042-7158.2010.01199.x, PMID 21039536.

Parkash V, Maan S, Deepika, Yadav SK, Hemlata, Jogpal V. Fast disintegrating tablets: opportunity in drug delivery system. J Adv Pharm Technol Res. 2011 Oct;2(4):223-35. doi: 10.4103/2231-4040.90877, PMID 22247889.

Maurya H, Kumar T. Formulation, standardization, and evaluation of polyherbal dispersible tablet. Int J App Pharm. 2019 Jan;11(1):158-67. doi: 10.22159/ijap.2019v11i1.30113.

Reddy MS, Setty M. Formulation and evaluation of dispersible tablets of sudarshan, vyswanara and panchasakar churnas. Res J Pharm Technol. 2011 Mar;4(3):380-4.

Arora S, Itankar P. Extraction, isolation and identification of flavonoid from Chenopodium album aerial parts. J Tradit Complement Med. 2018 Feb 6;8(4):476-82. doi: 10.1016/j.jtcme.2017.10.002, PMID 30302328.

Rodriguez De Luna SL, Ramirez Garza RE, Serna Saldivar SO. Environmentally friendly methods for flavonoid extraction from plant material: impact of their operating conditions on yield and antioxidant properties. Scientific World Journal. 2020 Aug 28;2020:6792069. doi: 10.1155/2020/6792069, PMID 32908461.

Chatwal GR, Anand SKJ. Instrumental methods of chemical analysis. 5th ed reprint. Mumbai: Himalaya Publishing House; 2021. p. 2.646-2.654.

Ozkan Y, Atay T, Dikmen N, Isimer A, Aboul-Enein HY. Improvement of water solubility and in vitro dissolution rate of gliclazide by complexation with beta-cyclodextrin. Pharm Acta Helv. 2000 Apr;74(4):365-70. doi: 10.1016/s0031-6865(99)00063-1, PMID 10812935.

Lima NGPB, Lima IPB, Aragao CFS. Compatibility studies of trioxsalen with excipients by DSC, DTA and FTIR. J Therm Anal Calorim. 2013 Mar;115(3):2111-318.

Shah RB, Tawakkul MA, Khan MA. Comparative evaluation of flow for pharmaceutical powders and granules. AAPS PharmSciTech. 2008 Feb;9(1):250-8. doi: 10.1208/s12249-008-9046-8, PMID 18446489.

Tiwari OP, Sharma M. Formulation and development of fast dissolving tablet of methanolic extract of some traditionally used medicinal plants for arthritis. Int J Appl Pharm Biol Res. 2017;8:28-32.

Maratha S, Sharma V, Walia V. Possible involvement of NO-cGMP signaling in the antidepressant-like effect of amantadine in mice. Metab Brain Dis. 2022 Aug;37(6):2067-75. doi: 10.1007/s11011-022-01006-4, PMID 35666396.

Rahayuningsih N, Fadhila SN, Cahyati KI. The antidepressant activity of Muntingia calabura L. Leaves ethanol extract in male swiss-webster mice. Int J App Pharm. 2022;14(4):58-63. doi: 10.22159/ijap.2022.v14s4.PP02.

Bernt E, Bergmeyer HU. l-glutamate UV-assay with gut amate dehydrogenase and NAD methods of enzymatic analysis. New York: Academic Press; 1974. p. 1704.

Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR. Analysis of nitrate, nitrite, and 15N nitrate in biological fluids. Anal Biochem. 1982 Oct;126(1):131-8. doi: 10.1016/0003-2697(82)90118-x, PMID 7181105.

Aulton ME. Dissolution and solubility. In: Aulton ME, Taylor MG. editors. Aulton’s Pharmaceutics, The design and manufacture of medicines. 5th ed. Amsterdam, Netherlands: Elsevier; 2018. p. 18-36.