CYTOTOXIC ACTIVITY OF TAXUS SUMATRANA (MIQ.) DE LAUB. BARK, LEAVES, AND SHOOTS ON HELA, T47D, AND MCF-7/HER2 CELL LINES

FATMA SRI WAHYUNI; DESI EKA PUTRI; YOZARWARDI USAMA PUTRA; DACHRIYANUS HAMIDI

doi:10.22159/ijap.2024.v16s1.23

Authors

FATMA SRI WAHYUNI Faculty of Pharmacy, Universitas Andalas, Padang, Indonesia 25163
DESI EKA PUTRI Indonesian Food and Drug Administration, Jakarta, Indonesia 10560. Post Graduate Student, Faculty of Pharmacy, Universitas Andalas, Padang, Indonesia 25163
YOZARWARDI USAMA PUTRA Dinas Kehutanan Provinsi Sumatera Barat, Padang, 25114
DACHRIYANUS HAMIDI Faculty of Pharmacy, Universitas Andalas, Padang, Indonesia 25163 https://orcid.org/0000-0002-1857-9001

DOI:

https://doi.org/10.22159/ijap.2024.v16s1.23

Keywords:

Cemara Sumatra, Breast cancer, Cervix cancer, MTT assay, Natural product

Abstract

Objective: Taxus sumatrana (Miq.) de Laub. (cemara Sumatra) is one of the plants found in Indonesia and other countries known as a medicine plant. Taxus's bark, leaves, and shoots are used traditionally and massively for some diseases (cancer, etc.), so recently it has become a rare plant. The chemical constituents of T. sumatrana are alkaloids, steroids, tannins, and flavonoids. This study aimed to investigate the potential anticancer properties of T. sumatrana bark, leaves, and shoot extracts.

Methods: The cytotoxic activity against the HELA, T47D, and MCF-7/HER2 cell lines was determined using the MTT assay. Each cell was cultured on 96 well plates treated with extract of T. sumatrana with concentrations of 100, 10, 1, and 0,1 µg/ml. Cells were incubated for 48 h at 37 °C, 5% CO₂ and then given 100 µl MTT solution 0.5 mg/ml in PBS (Phosphate Buffer Saline) for 4 h. The results of the measurements were processed with the GraphPad Prism Program.

Results: The bark, leaves, and shoots extracts have strong cytotoxic activity based on IC₅₀ parameters. The mean IC₅₀ of bark, leaves, and shoots on the HELA cell line consecutively 8.94; 5.93; and 4.08 μg/ml; on the T47D cell line 5.80, 4.86, and 4,11 μg/ml; and on MCF-7/HER2 cell line 7.46, 10.60, and 13.74 μg/ml).

Conclusion: T. sumatrana bark, leaves, and shoots have potential anti-cancer properties.

Downloads

Download data is not yet available.

References

Spjut RW. Taxonomy and nomenclature of taxus (Taxaceae). J Bot Res Inst Tex. 2007;1(1):203-89.

Frianto D. The distribution pattern and the density potential of Taxus sumatrana in Mount Tujuh, Kerinci District, Jambi. Pros Sem Nas Masy Biodiv Indon 2016;2:12-5.

Muhaimin M. Taxus sumatrana (Miq.) de Laub. obat anti Kanker Masa Depan. War Kebun, (Raya). 2017;14:11-20.

Wani MC, Horwitz SB. Nature as a remarkable chemist: a personal story of the discovery and development of Taxol®. Anticancer Drugs. 2014;25(5):482-7. doi: 10.1097/CAD.0000000000000063, PMID 24413390.

Food and Drug Administration/Center for Drug Evaluation and research C. summary of product characteristics: taxol® (paclitaxel) Injection; 2011.

WHO. World Health Organization 2023. Geneva: World Health Organization; 2023.

Kitagawa I, Mahmud T, Kobayashi M, Roemantyo H, Shibuya H. Taxol and its related taxoids from the needles of Taxus sumatrana. Chem Pharm Bull (Tokyo). 1995;43(2):365-7. doi: 10.1248/cpb.43.365, PMID 7728941.

Menlhk. Permen-Jenis-Satwa-dan-Tumbuhan-Dilindungi. Menteri Lingkung Hidup dan Kehutan Republik Indones; 2018. p. 1-30.

Shen YC, Wang SS, Pan YL, Lo KL, Chakraborty R, Chien Te CT. New taxane diterpenoids from the leaves and twigs of Taxus sumatrana. J Nat Prod. 2002;65(12):1848-52. doi: 10.1021/np0202273, PMID 12502326.

Shen YC, Pan YL, Lo KL, Wang SS, Chang YT, Wang LT. New taxane diterpenoids from Taiwanese Taxus sumatrana. Chem Pharm Bull (Tokyo). 2003;51(7):867-9. doi: 10.1248/cpb.51.867, PMID 12843599.

Shen YC, Cheng KC, Lin YC, Cheng YB, Khalil AT, Guh JH. Three new taxane diterpenoids from Taxus sumatrana. J Nat Prod. 2005;68(1):90-3. doi: 10.1021/np040132w, PMID 15679325.

Shen YC, Lin YS, Cheng Y, Cheng KC, Khalil AT, Kuo YH. Novel taxane diterpenes from Taxus sumatrana with the first C-21 taxane ester. Tetrahedron. 2005;61(5):1345-52. doi: 10.1016/j.tet.2004.10.110.

Shen YC, Hsu SM, Lin YS, Cheng KC, Chien Te CT, Chou CH. New bicyclic taxane diterpenoids from Taxus sumatrana. Chem Pharm Bull (Tokyo). 2005;53(7):808-10. doi: 10.1248/cpb.53.808, PMID 15997140.

Shen YC, Lin YS, Hsu SM, Khalil AT, Wang SS, Chien Te C. Tasumatrols PT, five new taxoids from Taxus sumatrana. Helv Chim Acta. 2007;90(7):1319-29. doi: 10.1002/hlca.200790133

Shen YC, Wang SS, Chien CT, Kuo YH, Khalil AT. Tasumatrols U-Z, Taxane diterpene esters from Taxus sumatrana. J Nat Prod. 2008;71(4):576-80. doi: 10.1021/np078016r, PMID 18220355.

Wang SS, Abd El Razek MH, Chen YC, Chien Te CT, Guh JH, Kuo YH. Abeo-taxane diterpenoids from the Taiwanese yew Taxus sumatrana. Chem Biodivers. 2009;6(12):2255-62. doi: 10.1002/cbdv.200900003, PMID 20020457.

Luh L, El-Razek M, Liaw C, Chen C, Lin Y, Kuo Y. Tri and bicyclic taxoids from the taiwanese yew taxus sumatrana. Helv Chim Acta. 2009;92(7):1349-58. doi: 10.1002/hlca.200900022.

Kuo WL, Chen FC, Chen KJ, Chen JJ. Taxusumatrin, a new taxoid from the stem bark of Taxus sumatrana. Chem Nat Compd. 2015;51(3):427-30. doi: 10.1007/s10600-015-1308-6.

Willson ML, Burke L, Ferguson T, Ghersi D, Nowak AK, Wilcken N. Taxanes for adjuvant treatment of early breast cancer. Cochrane Database Syst Rev. 2019;9(9):CD004421. doi: 10.1002/14651858.CD004421.pub3, PMID 31476253.

Holliday DL, Speirs V. Choosing correct breast cancer cell line for breast cancer research. Breast Cancer Res. 2011;13(4):1-7.

Dachriyanus SMV, Wahyuni FS, Wahyuni FS. +)-Isochimonanthine, a pyrrolidine indole alkaloid from argostemma yappii king. Aust J Chem. 2000;53(2):159-60. doi: 10.1071/CH99114.

Dachriyanus AD, Arbain DP, Putra DP, Sargent MV, Susila R, Wahyuni FS. Indole alkaloids from two species of Ophiorrhiza. Aust J Chem. 2000;53(3):221-4. doi: 10.1071/CH99112.

Arbain D, Byrne LT, Dachriyanus EN, Evrayoza N, Sargent MV. Bracteatine, a quaternary glucoalkaloid from Ophiorrhiza bracteata. Aust J Chem. 1997;50(11):1111-2. doi: 10.1071/C97055.

Arbain D, Byrne LT, Farmasi DJ, Sargent MV. Isomalindine-16-carboxylate, a zwitterionic alkaloid from Ophiorrhiza cf. communis. Aust J Chem. 1997;50(11):1109-10. doi: 10.1071/C97054.

Abubakar AR, Haque M. Preparation of medicinal plants: basic extraction and fractionation procedures for experimental purposes. J Pharm Bioallied Sci. 2020;12(1):1-10. doi: 10.4103/jpbs.JPBS_175_19, PMID 32801594.

Zhang QW, Lin LG, Ye WC. Techniques for extraction and isolation of natural products: a comprehensive review. Chin Med. 2018;13:20. doi: 10.1186/s13020-018-0177-x, PMID 29692864.

Leao P, Neves L, Colombo R, Shahab M, Oliveira J, Luz F. Temperature and storage periods on the maintenance of chemical composition of medicinal plants. ASB J. 2019;5(1):40. doi: 10.33158/ASB.2019v5i1p40.

Husni E, Nahari F, Wirasti Y, Wahyuni FS, Dachriyanus. Cytotoxicity study of ethanol extract of the stem bark of asam kandis (Garcinia cowa Roxb.) on T47D breast cancer cell line. Asian Pac J Trop Biomed. 2015;5(3):249-52. doi: 10.1016/S2221-1691(15)30013-7.

Wahyuni FS, Triastuti DH, Arifin H. Cytotoxicity study of ethanol extract of the leaves of asam kandis (Garcinia cowa Roxb.) on T47D breast cancer cell line. Phcog J. 2015;7(6):369-71. doi: 10.5530/pj.2015.6.9.

Wahyuni FS, Shaari K, Stanslas J, Lajis NH. Cytotoxic xanthones from the stem bark of Garcinia cowa Roxb. OMe 5a O. 2015;7(1):227-36.

Coecke S, Balls M, Bowe G, Davis J, Gstraunthaler G, Hartung T. Guidance on good cell culture practice: a report of the second ECVAM task force on good cell culture practice. Altern Lab Anim. 2005;33(3):261-87. doi: 10.1177/026119290503300313.

Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983;65(1-2):55-63. doi: 10.1016/0022-1759(83)90303-4, PMID 6606682.

Pieme CA, Kumar SG, Dongmo MS, Moukette BM, Boyoum FF, Ngogang JY. Antiproliferative activity and induction of apoptosis by Annona muricata (Annonaceae) extract on human cancer cells. BMC Complement Altern Med. 2014;14(1):516. doi: 10.1186/1472-6882-14-516, PMID 25539720.

Livingstone J. Natural compounds in cancer therapy. Int J Pharm Med. 2001;15(5). doi: 10.2165/00124363-200110000-00017.

Liebmann JE, Cook JA, Lipschultz C, Teague D, Fisher J, Mitchell JB. Cytotoxic studies of paclitaxel (Taxol) in human tumour cell lines. Br J Cancer. 1993;68(6):1104-9. doi: 10.1038/bjc.1993.488, PMID 7903152.

Vidensek N, Lim P, Campbell A, Carlson C. Taxol content in bark, wood, root, leaf, twig, and seedling from several taxus species. J Nat Prod. 1990;53(6):1609-10. doi: 10.1021/np50072a039, PMID 1982448.

Ismail U, Killeen RB. Taxane toxicity. StatPearls. 2023:1-8.