RETINOL STABILITY IN ANTI-AGING FACE SERUM POST-OPENING PERIOD

Authors

  • BAITHA PALANGGATAN MAGGADANI Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Indonesia, Depok-16424, West Java, Indonesia https://orcid.org/0000-0002-7284-0338
  • RISA RAHMAYATI Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Indonesia, Depok-16424, West Java, Indonesia
  • TAUFIQ INDRA RUKMANA Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Indonesia, Depok-16424, West Java, Indonesia https://orcid.org/0000-0003-3113-8291
  • CALLISTA ANDINIE MULYADI Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Indonesia, Depok-16424, West Java, Indonesia

DOI:

https://doi.org/10.22159/ijap.2024v16i4.50903

Keywords:

Retinol, Face serum, HPLC, Stability

Abstract

Objective: This study aimed to evaluate the stability of retinol in facial serum products under various storage conditions during a specific period after the product is opened. Retinol analysis will be conducted using reversed-phase high-performance liquid chromatography with a UV detector.

Methods: The optimum analytical method in this study was validated according to the ICH Q2 (R1) guideline. The chromatographic separation of retinol was achieved on RP-HPLC isocratic elution method at a wavelength of 324 nm, C18 column, mobile phase of methanol-acetonitrile (90:10), and a flow rate of 0.8 ml/min.

Results: The recovery value for this method was 98.06%-101.6,6%, with RSD ≤ 2%. Limit of Detection (LOD) and limit of Quantification (LOQ) values obtained were 1.1819 µg/ml and 3.9399 µg/ml. Determining retinol levels in face serum samples with variation in storage conditions was conducted on d 0, 7, 14, 21, and 30. The retinol levels in all storage conditions decreased over the 30 d, ranging from 25% to 79%. The highest decrease was observed in samples stored in transparent containers and exposed to light, with a reduction of 79% and an average weekly decrease of 17%.

Conclusion: Stability evaluation of retinol in the serum formulation showed decreased retinol concentration over time during storage. Transferring the product to a secondary container and exposure to light resulted in a higher concentration decrease than storing it in the original bottle.

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Published

07-07-2024

How to Cite

MAGGADANI, B. P., RAHMAYATI, R., RUKMANA, T. I., & MULYADI, C. A. (2024). RETINOL STABILITY IN ANTI-AGING FACE SERUM POST-OPENING PERIOD. International Journal of Applied Pharmaceutics, 16(4), 108–113. https://doi.org/10.22159/ijap.2024v16i4.50903

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