COMBINING LIQUID CHROMATOGRAPHY WITH TANDEM MASS SPECTROMETRY (LC-MS/MS) TECHNIQUE, SPECTROPHOTOMETERVALIDATION AND AN IN SILICO STUDY OF 96% ETHANOL EXTRACT OF SPIRULINA PLATENSIS

TIWUK SUSANTININGSIH; FADILAH FADILAH; ANI RETNO PRIJANTI; NOVI SILVIA HARDIANY

doi:10.22159/ijap.2024v16i5.51339

Authors

TIWUK SUSANTININGSIH Doctoral Program of Biomedical Science, Faculty of Medicine, Universitas Indonesia, Jakarta-10430, Indonesia.Department of Biochemistry Faculty of Medicine, UPN Veteran Jakarta, Jakarta-12450, Indonesia. https://orcid.org/0000-0002-5276-7997
FADILAH FADILAH Department of Medical Chemistry, Faculty of Medicine, University of Indonesia, Jakarta-10440, Indonesia https://orcid.org/0000-0002-8120-3138
ANI RETNO PRIJANTI Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Indonesia, Jakarta-10430, Indonesia.
NOVI SILVIA HARDIANY Magister Program of Biomedical Science, Faculty of Medicine, University of Indonesia, Jakarta-10430, Indonesia

DOI:

https://doi.org/10.22159/ijap.2024v16i5.51339

Keywords:

C-phycocyanin, in silico study, LC-MS/MS, spektrophotometer, Spirulina platensis

Abstract

Objective: This study was to analyze the component sofa 96% ethanol extract of Spirulina platensis by the LC-MS/MS technique, then validate them with the spectrophotometer technique using the C-phycocyanin standard and an in silico study approach as an antioxidant property of S. platensis against inflammatory.

Methods: Chromatographic resolution was attained with a Phenominex C18 (50 mm×2.6 mm, 3 µm) stationary column technique, validation using C-phycocyanin standard using the spectrophotometer technique, and an in silico study of c-phycocyanin using molecular docking analysis.

Results: Tentative active compounds such as flavonoid (Maltol and Morin), peptide (Cyclo Pro-Ala, Cyclo Pro-Pro, and Thymine), and phenol (m-Aminophenol, N-Methyltyramine, and Tyramine) have been identified from a 96% ethanol extract of S. platensis by LCMS/MS analysis. The concentration of c-phycocyanin in the 96% ethanol extract of S. platensis is 229, 2µg/ml. According to our in silico study, c-phycocyanin demonstrates potential as an anti-inflammatory agent.

Conclusion: The LC-MS/MS technique can detect flavonoid, peptide, and phenolic components in the 96% ethanol extract of S. platensis. A spectrophotometer can identify the validation equation of c-phycocyanin in a 96% ethanol extract of S. platensis. Based on our in silico study, c-phycocyanin demonstrate the capability to prevent inflammatory activity.

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