FORMULATION AND EVALUATION OF LICORICE OIL-BASED EMULGEL FOR THE TREATMENT OF PSORIASIS

Authors

DOI:

https://doi.org/10.22159/ijap.2024v16i5.51471

Keywords:

Licorice oil, Carbopol 934, Topical formulation, Psoriasis, Emulgel, HaCaT cell line

Abstract

Objective: The aim of the present research work was to develop and evaluate the topical emulgel incorporated with licorice oil for the effective management of psoriasis.

Methods: The present study involves the preparation and optimization of licorice oil-based emulsion using tween 80, span 20, propylene glycol and was loaded in gel base (carbopol 940 was used as gelling agent). The prepared emulgel were evaluated for various parameters such as particle size, zeta potential, entrapment efficiency, spreadibility, pH, viscosity, Fourier-Transform Infrared Spectroscopy (FTIR), in vitro release studies and in vitro cell line study.

Results: The optimized formulation was found to have droplet size of 54.50 nm,-14.1 V zeta potential, entrapment efficiency of 59.53±8.42 % and spreadibility of 2.901±0.12 mm. The pH and viscosity of optimized licorice oil-based emulgel was found to be 6.0±0.467 and 93,500±832 cps, respectively. Cumulative in vitro release was found to be 95.15±0.26 % has shown by the optimized formulation for 10 h. In (3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) MTT cytotoxicity study indicated non-toxic potential properties of licorice oil and its emulgel at lower level and caused moderate toxicity at higher level against Human Epidermal Keratinocytes (HaCaT) cell lines after the incubation period of 24 h respectively.

Conclusion: This study showed that the emulgel formulation has the potential to significantly enhance the efficacy of licorice oil in the treatment of psoriasis. These findings provide exciting new possibilities for improving psoriasis treatment and explores importance of continued research in this area.

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References

Sarac G, Koca TT, Baglan T. A brief summary of clinical types of psoriasis. North Clin Istanb. 2016 Jun 14;3(1):79-82. doi: 10.14744/nci.2016.16023, PMID 28058392, PMCID PMC5175084.

Urban K, Chu S, Giesey RL, Mehrmal S, Uppal P, Delost ME. Burden of skin disease and associated socioeconomic status in Asia: a cross-sectional analysis from the Global Burden of Disease Study 1990-2017. JAAD Int. 2021;2:40-50. doi: 10.1016/j.jdin.2020.10.006, PMID 34409353.

Danielsen K, Olsen AO, Wilsgaard T, Furberg AS. Is the prevalence of psoriasis increasing? A 30 y follow up of a population-based cohort. Br J Dermatol. 2013 Jun;168(6):1303-10. doi: 10.1111/bjd.12230, PMID 23374051.

Harden JL, Krueger JG, Bowcock AM. The immunogenetics of psoriasis: a comprehensive review. J Autoimmun. 2015 Nov;64:66-73. doi: 10.1016/j.jaut.2015.07.008, PMID 26215033, PMCID PMC4628849.

Kim WB, Jerome D, Yeung J. Diagnosis and management of psoriasis. Can Fam Physician. 2017 Apr;63(4):278-85. PMID 28404701, PMCID PMC5389757.

Merola JF, Tian H, Patil D, Richardson C, Scott A, Chen YH. Incidence and prevalence of psoriatic arthritis in patients with psoriasis stratified by psoriasis disease severity: retrospective analysis of an electronic health records database in the United States. J Am Acad Dermatol. 2022 Apr;86(4):748-57. doi: 10.1016/j.jaad.2021.09.019, PMID 34547358.

Chalmers R, O’Sullivan T, Owen CM, Griffiths CE. Withdrawn: interventions for guttate psoriasis. Cochrane Database Syst Rev. 2019;4(4):CD001213. doi: 10.1002/14651858.CD001213.pub2, PMID 30964200.

Micali G, Verzi AE, Giuffrida G, Panebianco E, Musumeci ML, Lacarrubba F. Inverse psoriasis: from diagnosis to current treatment options. Clin Cosmet Investig Dermatol. 2019 Dec 31;12:953-9. doi: 10.2147/CCID.S189000, PMID 32099435, PMCID PMC6997231.

Benjegerdes KE, Hyde K, Kivelevitch D, Mansouri B. Pustular psoriasis: pathophysiology and current treatment perspectives. Psoriasis (Auckl). 2016 Sep 12;6:131-44. doi: 10.2147/PTT.S98954, PMID 29387600, PMCID PMC5683122.

Singh RK, Lee KM, Ucmak D, Brodsky M, Atanelov Z, Farahnik B. Erythrodermic psoriasis: pathophysiology and current treatment perspectives. Psoriasis (Auckl). 2016;6:93-104. doi: 10.2147/PTT.S101232, PMID 28856115, PMCID PMC5572467.

Sato Y, Ogawa E, Okuyama R. Role of innate immune cells in psoriasis. Int J Mol Sci. 2020 Sep 9;21(18):6604. doi: 10.3390/ijms21186604, PMID 32917058, PMCID PMC7554918.

Mosca M, Hong J, Hadeler E, Brownstone N, Bhutani T, Liao W. Scalp psoriasis: a literature review of effective therapies and updated recommendations for practical management. Dermatol Ther (Heidelb). 2021 Jun;11(3):769-97. doi: 10.1007/s13555-021-00521-z, PMID 33893995, PMCID PMC8163911.

Bahadur S, Sharma M. Liposome based drug delivery for the management of psoriasis a comprehensive review. Curr Pharm Biotechnol. 2023;24(11):1383-96. doi: 10.2174/1389201024666221213144228, PMID 36518042.

Rendon A, Schakel K. Psoriasis pathogenesis and treatment. Int J Mol Sci. 2019 Mar 23;20(6):1475. doi: 10.3390/ijms20061475, PMID 30909615, PMCID PMC6471628.

Wahab S, Annadurai S, Abullais SS, Das G, Ahmad W, Ahmad MF. Glycyrrhiza glabra (licorice): a comprehensive review on its phytochemistry biological activities, clinical evidence and toxicology. Plants (Basel). 2021 Dec 14;10(12):2751. doi: 10.3390/plants10122751, PMID 34961221, PMCID PMC8703329.

Tiwari A, Tiwari A. A comparative study of polyphenolic content in acacia catechu bark extracts and bibliographic analysis with reference to Guna (Madhya Pradesh) India. Asian J Pharm Clin Res. 2024 Jun 7;17(6):45-50. doi: 10.22159/ajpcr.2024,v17i6.50809.

Chhabra R, Sharma R, Kaur A, Sharma VK, Thakur S. Phytochemical characterization and antifungal ability of three meliaceae species against Bipolaris oryzae. Agric Res J. 2023 May;60(1):146-52. doi: 10.5958/2395-146X.2023.00023.6.

Fiore C, Eisenhut M, Ragazzi E, Zanchin G, Armanini D. A history of the therapeutic use of liquorice in europe. J Ethnopharmacol. 2005 Jul 14;99(3):317-24. doi: 10.1016/j.jep.2005.04.015, PMID 15978760, PMCID PMC7125727.

Pastorino G, Cornara L, Soares S, Rodrigues F, Oliveira MB. Liquorice (Glycyrrhiza glabra): a phytochemical and pharmacological review. Phytother Res. 2018 Dec;32(12):2323-39. doi: 10.1002/ptr.6178, PMID 30117204, PMCID PMC7167772.

Gaur R, Yadav KS, Verma RK, Yadav NP, Bhakuni RS. In vivo anti-diabetic activity of derivatives of isoliquiritigenin and liquiritigenin. Phytomedicine. 2014 Mar 15;21(4):415-22. doi: 10.1016/j.phymed.2013.10.015, PMID 24262065.

Wahab S, Annadurai S, Abullais SS, Das G, Ahmad W, Ahmad MF. Glycyrrhiza glabra (licorice): a comprehensive review on its phytochemistry biological activities clinical evidence and toxicology. Plants (Basel). 2021 Dec 14;10(12):2751. doi: 10.3390/plants10122751, PMID 34961221, PMCID PMC8703329.

Rizzato G, Scalabrin E, Radaelli M, Capodaglio G, Piccolo O. A new exploration of licorice metabolome. Food Chem. 2017 Apr 15;221:959-68. doi: 10.1016/j.foodchem.2016.11.068, PMID 27979300.

Cerulli A, Masullo M, Montoro P, Piacente S. Licorice (Glycyrrhiza glabra, G. uralensis, and G. inflata) and their constituents as active cosmeceutical ingredients. Cosmetics. 2022 Jan 5;9(1):7. doi: 10.3390/cosmetics9010007.

Desai J, Patel RJ, Desai D. Investigation on potential of karanjin loaded emulgel for improved efficacy against psoriasis. Ind J Pharm Edu Res. 2023 Mar 2;57(2):393-400. doi: 10.5530/ijper.57.2.49.

Malavi S, Kumbhar P, Manjappa A, Disouza J, Dwivedi J. Emulgel for improved topical delivery of tretinoin: formulation design and characterization. Ann Pharm Fr. 2022 Mar;80(2):157-68. doi: 10.1016/j.pharma.2021.05.004, PMID 34029557.

Sharma A, Singh AP, Harikumar SL. Development and optimization of nanoemulsion based gel for enhanced transdermal delivery of nitrendipine using box behnken statistical design. Drug Dev Ind Pharm. 2020 Feb;46(2):329-42. doi: 10.1080/03639045.2020.1721527, PMID 31976777.

He E, Li H, Li X, Wu X, Lei K, Diao Y. Transdermal delivery of indirubin loaded microemulsion gel: preparation characterization and anti-psoriatic activity. Int J Mol Sci. 2022 Mar 30;23(7):3798. doi: 10.3390/ijms23073798, PMID 35409158, PMCID PMC8998921.

Yadav R, Pandey NK, Kukkar R. Design development and improvement of an emulgel containing silver nanoparticles and vitamin D3 for its potential to accelerate the healing of wound. Int J App Pharm. 2014 May 7;16(3):149-58. doi: 10.22159/ijap.2024v16i3.50344.

Bhardwaj S, Gaur PK, Tiwari A. Development of topical nanoemulgel using combined therapy for treating psoriasis. Assay Drug Dev Technol. 2022 Jan;20(1):42-54. doi: 10.1089/adt.2021.112, PMID 34883035.

Cao J, Jiang M, Hua S, Yang L, Li P. Application of gas chromatography and gas chromatography mass spectrometry in quality control of chinese medicines. Springer; 2024 Apr 27. p. 451-74. doi: 10.1007/978-981-99-9871-5_14.

Sarikurkcu C, Sabih Ozer M, Cakir A, Eskici M, Mete E. GC/MS evaluation and in vitro antioxidant activity of essential oil and solvent extracts of an endemic plant used as folk remedy in turkey: phlomis bourgaei boiss. Evid Based Complement Alternat Med. 2013;2013:293080. doi: 10.1155/2013/293080, PMID 23762120, PMCID PMC3666358.

Mittal S, Ali J, Baboota S. Enhanced anti-psoriatic activity of tacrolimus loaded nanoemulsion gel via omega 3-fatty acid (EPA and DHA) rich oils-fish oil and linseed oil. J Drug Deliv Sci Technol. 2021 Mar 4;63:102458. doi: 10.1016/j.jddst.2021.102458.

Kaur D, Raina A, Singh N. Formulation and evaluation of carbopol 940 based glibenclamide transdermal gel. Int J Pharm Pharm Sci. 2014 Aug 8;6(8):434-40:ISSN-0975-1491.

Alam MS, Algahtani MS, Ahmad J, Kohli K, Shafiq-Un-Nabi S, Warsi MH. Formulation design and evaluation of aceclofenac nanogel for topical application. Ther Deliv. 2020 Dec;11(12):767-78. doi: 10.4155/tde-2020-0076, PMID 33225842.

Soliman WE, Shehata TM, Mohamed ME, Younis NS, Elsewedy HS. Enhancement of curcumin anti-inflammatory effect via formulation into myrrh oil based nanoemulgel. Polymers (Basel). 2021 Feb 14;13(4):577. doi: 10.3390/polym13040577, PMID 33672981, PMCID PMC7917777.

Nurleni N, Firdiawan A, Rendowaty A, Kurniasari R. Formulation and evaluation of red ginger rhizome extract soap as an antibacterial. Int J App Pharm. 2024 May 3;16(3):251-5. doi: 10.22159/ijap.2024v16i3.49550.

Pintea A, Vlad RA, Antonoaea P, Redai EM, Todoran N, Barabas EC. Structural characterization and optimization of a miconazole oral gel. Polymers (Basel). 2022 Nov 18;14(22):5011. doi: 10.3390/polym14225011, PMID 36433136, PMCID PMC9692734.

Khullar R, Kumar D, Seth N, Saini S. Formulation and evaluation of mefenamic acid emulgel for topical delivery. Saudi Pharm J. 2012 Jan;20(1):63-7. doi: 10.1016/j.jsps.2011.08.001, PMID 23960777, PMCID PMC3745000.

Said Dos Santos R, Bassi da Silva J, Rosseto HC, Vecchi CF, Campanholi KD, Caetano W. Emulgels containing propolis and curcumin: the effect of type of vegetable oil poly(acrylic acid) and bioactive agent on physicochemical stability mechanical and rheological properties. Gels. 2021 Aug 12;7(3):120. doi: 10.3390/gels7030120, PMID 34449614, PMCID PMC8396026.

Arora R, Aggarwal G, Harikumar SL, Kaur K. Nanoemulsion based hydrogel for enhanced transdermal delivery of ketoprofen. Adv Pharm. 2014;2014:1-12. doi: 10.1155/2014/468456.

Dantas MG, Reis SA, Damasceno CM, Rolim LA, Rolim Neto PJ, Carvalho FO. Development and evaluation of stability of a gel formulation containing the monoterpene borneol. Scientific World Journal. 2016;2016:7394685. doi: 10.1155/2016/7394685, PMID 27247965, PMCID PMC4876256.

Burki IK, Khan MK, Khan BA, Uzair B, Braga VA, Jamil QA. Formulation development characterization and evaluation of a novel dexibuprofen capsaicin skin emulgel with improved in vivo anti-inflammatory and analgesic effects. AAPS PharmSciTech. 2020 Jul 31;21(6):211. doi: 10.1208/s12249-020-01760-7, PMID 32737606.

Rompicherla NC, Joshi P, Shetty A, Sudhakar K, Amin HI, Mishra Y. Design formulation and evaluation of aloe vera gel-based capsaicin transemulgel for osteoarthritis. Pharmaceutics. 2022 Aug 29;14(9):1812. doi: 10.3390/pharmaceutics14091812, PMID 36145560, PMCID PMC9503439.

Agrawal M, Saraf S, Pradhan M, Patel RJ, Singhvi G, Ajazuddin. Design and optimization of curcumin loaded nano lipid carrier system using box-behnken design. Biomed Pharmacother. 2021;141:111919. doi: 10.1016/j.biopha.2021.111919, PMID 34328108.

Abdallah MH, Elghamry HA, Khalifa NE, Khojali WM, Khafagy ES, Shawky S. Development and optimization of erythromycin loaded transethosomes cinnamon oil based emulgel for antimicrobial efficiency. Gels. 2023 Feb 6;9(2):137. doi: 10.3390/gels9020137, PMID 36826307, PMCID PMC9956959.

Gottemukkula LD, Pathuri R. Development and optimization of a dolutegravir nanosuspension using box behnken design. Int J App Pharm. 2024 May 3;16(3):129-39. doi: 10.22159/ijap.2024v16i3.50315.

Jothula H, Navuluri S, Mulakayala NR. Stability based HPLC method for cyclophosphamide related substances in finished drug products: development and validation. Int J Curr Pharm Sci. 2024 May 3;16(3):42-51. doi: 10.22159/ijcpr.2024v16i3.4061.

Singh S, Aldawsari HM, Alam A, Alqarni MH, Ranjan S, Kesharwani P. Synthesis and antimicrobial activity of vancomycin conjugated zinc coordination polymer nanoparticles against methicillin resistant staphylococcus aureus. J Drug Deliv Sci Technol. 2022 Apr 1;70:103255. doi: 10.1016/j.jddst.2022.103255.

Mulye SP, Wadkar KA, Kondawar MS. Formulation development and evaluation of indomethacin emulgel. Pharm Sin. 2013 Jan 1;4(5):31-45.

Adewale OO, Oyelola RF, Oladele JO, Agbaje WB. Assessing the ability of polysaccharides extracted from date palm fruit to salvage wistar rats from cisplatin linked hepatic damage. Pharmacological Research Modern Chinese Medicine. 2024;11. doi: 10.1016/j.prmcm.2024.100400.

Yadav JP, Verma A, Pathak P, Kumar V, Patel DK. Wound healing antidiabetic and antioxidant activity of Neolamarckiacadamba quercetin rich, extract. pharmacological research modern. Chin Med. 2024 Mar 26;11;2667-1425:100417. doi: 10.1016/j.prmcm.2024.100417.

Xin Y, Yun S, Yuhe L, Yinxue M, Shurui N, Yue Z. Development of licorice flavonoids loaded microemulsion for transdermal delivery using CCD-optimal experimental approach: formulation development and characterization. Front Nanotechnol. 2021 Nov 18;3:748791. doi: 10.3389/fnano.2021.748791.

Azeem A, Rizwan M, Ahmad FJ, Iqbal Z, Khar RK, Aqil M. Nanoemulsion components screening and selection: a technical note. AAPS PharmSciTech. 2009;10(1):69-76. doi: 10.1208/s12249-008-9178-x, PMID 19148761, PMCID PMC2663668.

Arshad W, Khan HM, Akhtar N, Mohammad IS. Polymeric emulgel carrying Cinnamomum tamala extract: promising delivery system for potential topical applications. Braz J Pharm Sci. 2020;56:1-11. doi: 10.1590/s2175-97902019000418318.

Shinde U, Pokharkar S, Modani S. Design and evaluation of microemulsion gel system of nadifloxacin. Indian J Pharm Sci. 2012 May;74(3):237-47. doi: 10.4103/0250-474X.106066, PMID 23439454, PMCID PMC3574534.

Mulye SP, Wadkar KA, Kondawar MS. Formulation development and evaluation of indomethacin emulgel. Pharm Sin. 2013 Jan;4(5):31-45.

Arabpoor B, Yousefi S, Weisany W, Ghasemlou M. Multifunctional coating composed of Eryngium campestre L. essential oil encapsulated in nano chitosan to prolong the shelf life of fresh cherry fruits. Food Hydrocoll. 2021 Feb;111:106394. doi: 10.1016/j.foodhyd.2020.106394.

Burki IK, Khan MK, Khan BA, Uzair B, Braga VA, Jamil QA. Formulation development characterization and evaluation of a novel dexibuprofen capsaicin skin emulgel with improved in vivo anti-inflammatory and analgesic effects. AAPS PharmSciTech. 2020 Jul 31;21(6):211. doi: 10.1208/s12249-020-01760-7, PMID 32737606.

Joseph E, Singhvi G. Multifunctional nanocrystals for cancer therapy: a potential nanocarrier. Nanomater Drug Deliv Ther. 2019 Jan 1:91-116. doi: 10.1016/B978-0-12-816505-8.00007-2.

Kumar R. Lipid based nanoparticles for drug delivery systems. Nanocarriers Drug Deliv Nanosci Nanotechnol Drug Deliv. 2018:249-84. doi: 10.1016/B978-0-12-814033-8.00008-4.

Ali Khan B, Ullah S, Khan MK, Alshahrani SM, Braga VA. Formulation and evaluation of Ocimum basilicum based emulgel for wound healing using animal model. Saudi Pharm J. 2020 Dec;28(12):1842-50. doi: 10.1016/j.jsps.2020.11.011, PMID 33424273, PMCID PMC7783209.

Gabrielli S, Pastore G, Stella F, Marcantoni E, Sarasini F, Tirillo J. Chemical and mechanical characterization of licorice root and palm leaf waste incorporated into poly(urethane-acrylate) (PUA). Molecules. 2021 Dec 19;26(24):7682. doi: 10.3390/molecules26247682, PMID 34946764, PMCID PMC8705998.

Tang J, Luan F, Chen X. Binding analysis of glycyrrhetinic acid to human serum albumin: fluorescence spectroscopy FTIR and molecular modeling. Bioorg Med Chem. 2006 May 1;14(9):3210-7. doi: 10.1016/j.bmc.2005.12.034, PMID 16412649.

Abdallah MH, Elghamry HA, Khalifa NE, Khojali WM, Khafagy ES, Shawky S. Development and optimization of erythromycin loaded transethosomes cinnamon oil based emulgel for antimicrobial efficiency. Gels. 2023 Feb 6;9(2):137. doi: 10.3390/gels9020137, PMID 36826307, PMCID PMC9956959.

Nikumbh KV, Sevankar SG, Patil MP. Formulation development in vitro and in vivo evaluation of microemulsion based gel loaded with ketoprofen. Drug Deliv. 2015;22(4):509-15. doi: 10.3109/10717544.2013.859186, PMID 24266589.

Reena K, Mittal S, Faizan M, Jahan I, Rahman Y, Khan R. Enhancement of curcumins anti-psoriatic efficacy via formulation into tea tree oil-based emulgel. Gels. 2023 Dec 13;9(12):973. doi: 10.3390/gels9120973, PMID 38131959, PMCID PMC10743130.

Azam F, Alqarni MH, Alnasser SM, Alam P, Jawaid T, Kamal M. Formulation in vitro and in silico evaluations of anise (Pimpinella anisum L.) essential oil emulgel with improved antimicrobial effects. Gels. 2023 Jan 28;9(2):111. doi: 10.3390/gels9020111, PMID 36826281, PMCID PMC9957046.

Yao Q, Chang B, Wu L, Gao Y. Antibacterial and anti-inflammatory properties of ethanol extract of Melicope pteleifolia an ethnomedicine in Southwest China. Pharmacological research. Mod Chin Med. 2024 Jan 14;10:100360. doi: 10.1016/j.prmcm.2024.100360.

Gerlier D, Thomasset N. Use of MTT colorimetric assay to measure cell activation. J Immunol Methods. 1986 Nov 20;94(1-2):57-63. doi: 10.1016/0022-1759(86)90215-2, PMID 3782817.

Akila RM, Janani M. Development characterization and evaluation of the antimicrobial properties of biodegradable porous scaffolds loaded with natural vanillin. Int J Pharm Pharm Sci. 2023 Nov 11;15:31-7. doi: 10.22159/ijpps.2023v15i11.48987.

Published

07-09-2024

How to Cite

JUREL, P., BAHADUR, S., & BAJPAI, M. (2024). FORMULATION AND EVALUATION OF LICORICE OIL-BASED EMULGEL FOR THE TREATMENT OF PSORIASIS. International Journal of Applied Pharmaceutics, 16(5), 165–175. https://doi.org/10.22159/ijap.2024v16i5.51471

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