SCREENING OF PHYTOCHEMICALS AND ANTIMICROBIAL POTENTIAL OF EXTRACTS OF VETIVER ZIZANOIDES AND PHRAGMITES KARKA AGAINST CLINICAL ISOLATES

Amrita Soni; Praveen Dahiya

doi:10.22159/ijap.2015v7i1.7100

Authors

Amrita Soni Amity Institute of Biotechnology, Amity University-Uttar Pradesh, Noida-201303, Uttar Pradesh, India
Praveen Dahiya Amity Institute of Biotechnology, Amity University-Uttar Pradesh, Noida-201303, Uttar Pradesh, India

DOI:

https://doi.org/10.22159/ijap.2015v7i1.7100

Keywords:

Agar well diffusion, Clinical isolates, Phragmites karka, Phytochemical analysis, TLC bioautography, Vetiveria zizanoides

Abstract

Objective: To screen the phytochemicals present in extracts of Vetiver zizanoides and Phragmites karka. The antimicrobial potential was also determined against selected clinical isolates.

Methods: The antimicrobial potential of extracts from Vetiver zizanoides and Phragmites karka was evaluated by agar well diffusion method against selected clinical isolates. Preliminary phytochemical analysis and TLC-bioautographic method was also performed.

Results: Antimicrobial activity of Vetiver zizanoides and Phragmites karka extracts was assessed on nine bacterial and four fungal clinical isolates. The solvent extracts from both the grasses showed maximum activity against S. aureus 1, producing the maximum zone of inhibition 15Â±0.12 mm in Vetiveria zizanoides ethanol extract and 13Â±0.09 mm in diethyl ether extract of Phragmites karka. Preliminary phytochemical analysis demonstrated the presence of most of the phytochemicals including flavonoids, saponins, cardiac glycosides, tannins, alkaloids and steroids in both methanol and diethyl ether extracts or in any of them. Thin layer chromatography and bioautography assay in Vetiveria zizanoides methanol extracts demonstrated well defined growth inhibition zones against S. aureus 1 in correspondence with flavonoids observed at Rf value ranging from 0.63-0.75.

Conclusion: The present study opens future prospects of these plant constituents to be used as potential antimicrobial drug against infectious agents and can be used in treatment of various infectious diseases.

Keywords: Agar well diffusion, Clinical isolates, Phragmites karka, Phytochemical analysis, TLC bioautography, Vetiveria zizanoides.

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References

Gaikwad RS, Rajendra BK, Kulkarni AU, Gaikwad DR, Panchal VH. In vitro antimicrobial activity of crude extracts of Jatropha species. Curr Bot 2012;3:09-15.

Yoganarasimhan SN. Medicinal plants of India. Karnataka: Interline Publishing; 1996;1:359.

Barad R, Atodariya U, Bhatt S, Patel H, Upadhyay S, Upadhyay U. Antibacterial and preliminary cytotoxic activity of the roots of Vetiveria Zizaniodes. Int J Pharm Rev Res 2013;3:23-5.

Balasankar D, Vanilarasu K, Preetha PS, Rajeswari S, Umadevi M, Bhowmik D. Traditional and medicinal uses of Vetiver. J Med Plants Studies 2013;1:191-200.

Luqman S. Investigations on biological activity of Vetiveria zizanioides L. Nash, a palingenesis of some important findings in miracle grass. Nature Proceedings; 2012. Available from: http://dx.doi.org/10.1038/npre.2012.6904.1

Bobbarala VV, Katikala PK, Naidu KC, Penumajji S. Antifungal activity of selected plant extracts against phytopathogenic fungi Aspergillus niger F2723. Indian J Sci Technol 2009;2:6839-46.

Dahiya P, Purkayastha S. Phytochemical screening and antimicrobial activity of some medicinal plants against multi-drug resistant bacteria from clinical isolates. Indian J Pharm Sci 2012a;74:443-50.

Trease GE, Evans WC. Pharmacognosy. 13th ed. London (UK): ELBS Oxford University Press; 1989.

Sofowora A. Medicinal plants and traditional medicine in Africa. 2nd ed. Ibadan (Nigeria): Spectrum Books Ltd; 1993.

Dahiya P, Purkayastha S. Phytochemical analysis and antibacterial efficacy of Dill seed oil against multi drug resistant clinical isolates. Asian J Pharm Clin Res 2012b:5:62-4.

Putiyanan S, Khesorn N, Manasnant B, Banyong K, Chantana K. Pharmacognostic identification and antimicrobial activity evaluation of Vetiveria Zizanioides (L.) Nash. ex small root. CMUJ 2005;4:299-303.

Devi SV, Kumar KA, Maheswari MU, Shanmugam ATS, Anand RS. In vitro antibacterial activity of ethanolic extract of Vetiveria Zizaniodes root. Int J Pharm Sci Res 2010;1:120-4.

Sangeetha D, Stella D. Screening of antimicrobial activity of Vetiver extracts against certain pathogenic microorganisms. Int J Pharm Biol Arch 2012;3:197-03.

Devprakash KK, Srinivasan T, Subburaju, Singh SS. Antimicrobial activity of alcoholic and aqueous extracts of Vetiveria zizanioides. J Pharm Res 2011;4:1343-4.

Ratha M, Subha K, Senthilkumar G, Panneerselvam A. Screening of phytochemical and antibacterial activity of Hemidesmus indicus (L.) and Vetiveria zizanoides (L.). Eur J Exp Biol 2012;2:363-8.

Subhadradevi V, Asokkumar K, Umamaheaswari M, Sivashanmugam A, Sankaranand R. In vitro antioxidant activity of Vetiveria Zizanioides root extract. Tanzania J Health Res 2010;12:276-81.

Mishra S, Sharma SK, Mohapatra S, Chauhan D. An overview on Vetiveria Zizanioides. Res J Pharm Biol Chem Sci 2013;4:777-80.

Basari DF, Fan SH. The potential of aqueous and acetone extracts of galls of Quercus infectoria as bacterial agents. Indian J Pharmacol 2009;1:26-9.