QUALITATIVE AND QUANTITATIVE PHYTOCHEMICAL ANALYSIS AND BACTERICIDAL ACTIVITY OF PELARGONIUM GRAVEOLENS L'HER
DOI:
https://doi.org/10.22159/ijap.2016v8i3.9075Keywords:
Phytochemical screening, Primary and secondary metabolites, Antibacterial screening, Pelargonium graveolensAbstract
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Objective: The present study investigates the qualitative and quantitative phytoconstituents and bactericidal effect of medicinally important Pelargonium graveolens L' Her leaves extracts.
Methods: Preliminary phytochemical screening analyses were determined using standard protocol methods. In addition, antibacterial activities of the P. graveolens leaves extracts were evaluated by disc diffusion method against Gram-positive and Gram-negative bacteria.
Results: The results revealed that ethanolic extract of P. graveolens was found to be the predominant occurrence of phytochemicals (9/11) which contains flavonoid, phenol, tannins, saponins, reducing sugar, glycosides, terpenoids, anthraquinone, and phlorotannins while starch and steroids were absent. In quantitative estimation of bioactive phytoconstituents showed carbohydrates (74 mg/gdw), protein (41.25 mg/gdw), chlorophyll (2.2±0.05 mg/gdw), lipids (0.07 mg/gdw), tannins (135.3 gm/gdw), phenolic compounds (123.75 mg/gdw) and flavonoids (50 mg/gdw) were found to have higher amount in ethanolic extract followed by acetone, methanol and aqueous extract and also showed an inhibitory action on growth of tested bacteria.
Conclusion: Ethanolic extract of the P. graveolens leaves hold promises as a potential source of pharmaceutically important phytochemicals and also have strong antibacterial activity against Staphylococcus aureus, Escherichia coli, Pseudomonas aeroginosa and Klebsiella pneumonia.
Keywords: Phytochemical screening, Primary and secondary metabolites, Antibacterial screening, Pelargonium graveolens
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