PRELIMINARY PHYTOCHEMICAL SCREENING AND EVALUATION OF ANALGESIC AND MUSCLE RELAXANT ACTIVITY OF THE ETHANOLIC EXTRACT OF THE LEAVES OF MIRABILIS JALAPA
DOI:
https://doi.org/10.22159/ijcpr.2017v9i5.22144Keywords:
Mirabilis jalapa, Phytochemical screening, Analgesic, Muscle relaxant, Diclofenac sodium, LorazepamAbstract
Objective: The aim and objectives of the present work is to determine pharmacological activity upon ethnopharmacological survey. The present study deals with phytochemical screening and analgesic as well as muscle relaxant activity of leaves of Mirabilis jalapa.
Methods: The present study is aimed at phytochemical screening and evaluating the analgesic and muscle relaxant activities of ethanolic leaf extract of Mirabilis jalapa by using hot plate method and rota rod method respectively [1, 2]. The Phytochemical screening of the extract was done according to the standard procedures to reveal the presence of the active constituents like Alkaloids, flavonoids, phenols, glycosides, tannins, saponins, steroids, carbohydrates etc [3-5].
Results: The analgesic and muscle relaxant activity study were dose dependent. The EEMJ extracts (100 mg/kg, 200 mg/kg, 500 mg/kg) and the standard drug Diclofenac sodium (25 mg/kg) shows significant increase in the reaction time when compared with control at 30 min, 60 min, 90 min and 120 min and the effect of standard was found to be highest during the study. Another study was designed to evaluate the skeletal muscle relaxant properties of ethanolic extract of leaves of Mirabilis jalapa. Linn by taking the EEMJ extracts (100 mg/kg, 200 mg/kg, 500 mg/kg) and standard drug Lorzepam (10 mg/kg). Both the extracts and standard drug show decrease in the fall of time in a dose dependent manner when compared with control at 15 min, 30 min, 45 min respectively.
Conclusion: Therefore, from the above study it is revealed that Mirabilis jalapa showing better pharmacological activities (Analgesic and Muscle relaxant) in dose dependent manner.
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Cartmell SM, Gelgor L, Mitchell D. A revised rota rod procedure for measuring the effect of antinociceptive drugs on motor function in the rat. J Pharmacol Meth 1991;26:149–59.
Lekshmi RN, Manjunath KP, Savadi RV, Akki KS. Antiinflammatory activity of Mirabilis jalapa Linn leaves. J Basic Clin Pharm 2010;1:93-6.
Kokate CK, Purohit AP, Gokhale SB. Pharmacognosy. 8th ed. India: Nirali Prakashan; 2002. p. 154-272.
Evans WC, Trease, Evans. Pharmacognosy. 15th ed. Toronto: Harcourt Pub Ltd; 2002. p. 1-40.
Khandelwal KR. Practical pharmacognosy techniques and experiments. India: Nirali Prakashan; 2010. p. 12, 30-12, 32.
Adimoelja A. Phytochemicals and breakthrough of traditional herbs in the management of sexual dysfunction. Int J Androl 2000;23:82-4.
Akerele O, Olayiwola V. Nature’s medicinal bounty: don’t throw it away. W H Forum 1993;2:390-5.
Colixto JB, Rafeeq AK, Ahmed S, Aslam M. Efficacy, safty, quality control, marketing and regulatory guidelines for herbal medicines. Braz J Med Biol Res 2000;33:179-89.
Adeolu A, Sunday OO. Anti-inflammatory and analgesic activities of the soft drink leaf extract of Phyllanthus amarus in some laboratory animals. Br Biotechnol J 2013;3:191-204.
Lee SH, Han CD, Yang IH, Ha CW. Prescription pattern of NSAIDs and the prevalence of NSAID-induced gastrointestinal risk factors of orthopedic patients in clinic practice in Kore. J Korean Med Sci 2011;26:561-7.
Raghavendra T. Neuromuscular blocking drugs: discovery and development. J Royal Soc Med 2002;95:363-7.
Ncube NS, Afolayan AJ, Okoh AI. Assessment techniques of antimicrobial properties of natural compounds of plant origin: current methods and future trends. Afr J Biol 2008;7:1797-806.
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