THE CURATIVE EFFECT OF METHYLSULFONYLMETHANE ON AN EXPERIMENTAL MODEL OF ULCERATIVE COLITIS IN RATS
DOI:
https://doi.org/10.22159/ijcpr.2019v11i3.34101Keywords:
MSM, Ulcerative colitis, Glutathione, MacroscopicAbstract
Objective: Ulcerative colitis is a chronic inflammatory bowel disease of unknown etiology that affects the mucosa and submucosa of the colon and rectum. It is associated with oxidative stress, neutrophil infiltration and production of inflammatory mediators. In the present study, the anti-inflammatory and antioxidant effects of Methylsulfonylmethane (MSM) in treatment the acetic acid (A) induced model for UC in rats were examined.
Methods: Ulcerative colitis was induced by intra colonic administration of 2 ml of 4% acetic acid in adult male Wistar rats which were divided into 3 groups: normal control (N), acetic acid (A) control, and MSM (1000 mg/kg). Rats received treatment for six consecutive days. On day 7, the rats were sacrificed, the colon was removed, the body weight, colon weight/length ratio, tissue glutathione (GSH) concentration, and macroscopic evaluations, were performed.
Results: The colon weight/length ratio was decreased significantly (P<0.05). The glutathione (GSH) concentration was increased significantly (P<0.05). The macroscopic parameters were decreased, but it didn’t reduce significantly in the MSM treated groups compared to group A. Contrariwise the parameters of group A. The levels of body weight were increased, and colon weight/length ratio was decreased significantly (P<0.05). The glutathione (GSH) concentration was increased significantly (P<0.05). The macroscopic parameters were decreased significantly in the MSM treated group, compared to the acetic acid-treated group.
Conclusions: Methylsulfonylmethane is an effective, safety natural product with little side effects, has a good curative effect in a dose (1000 mg/kg) on experimental Ulcerative colitis induced by acetic acid.
Downloads
References
2. Hagar HH, El Medany A, El Eter E, Arafa M. Ameliorative effect of pyrrolidinedithiocarbamate on acetic acid-induced colitis in rats. Eur J Pharmacol 2007;554:69-77.
3. Tuzun A, Erdil A, I?nal V, Ayd?n A, Bagc? S, Ye?ilova Z, et al. Oxidative stress and antioxidant capacity in patients with inflammatory bowel disease. Clin Biochem 2002;35:569-72.
4. Medhi B, Prakash A, Avti P, Saikia U, Pandhi P, Khanduja K. Effect of manuka honey and sulfasalazine in combination to promote antioxidant defense system in experimentally induced ulcerative colitis model in rats. Indian J Exp Biol 2008;46:583.
5. Debbi EM, Agar G, Fichman G, Ziv YB, Kardosh R, Halperin N, et al. Efficacy of methylsulfonylmethane supplementation on osteoarthritis of the knee: a randomized controlled study. BMC Complementary Alternative Med 2011;11:1.
6. Ebisuzaki K. Aspirin and methylsulfonylmethane (MSM): a search for common mechanisms, with implications for cancer prevention. Anticancer Res 2002;23(1A):453-8.
7. Al Bitar V, Al laham S. Methylsulfonylmethane and green tea extract reduced oxidative stress and inflammation in an ulcerative colitis. Asian J Pharm Clin Res 2013;6:153-8.
8. Minaiyan M, Asghari G, Taheri D, Saeidi M, Nasr Esfahani S. Anti-inflammatory effect of moringa oleifera lam. seeds on acetic acid-induced acute colitis in rats. Avicenna J Phytomed 2014;4:127-36.
9. Amirshahrokhi K, Bohlooli S, Chinifroush M. The effect of methylsulfonylmethane on the experimental colitis in the rat. Toxicol Appl Pharmacol 2011;253:197-202.
10. Blenn C, Althaus FR, Malanga M. Poly (ADP-ribose) glycohydrolase silencing protects against H2O2-induced cell death. Biochem J 2006;396:419-29.
11. Nagib MM, Tadros MG, ELSayed MI, Khalifa AE. Anti-inflammatory and anti-oxidant activities of olmesartan medoxomil ameliorate experimental colitis in rats. Toxicol Appl Pharmacol 2013;271:106-13.
12. Kondamudi PK, Kovelamudi H, Mathew G, Nayak PG, Rao MC, Shenoy RR. Investigation of sesamol on myeloperoxidase and colon morphology in acetic acid-induced inflammatory bowel disorder in albino rats. Sci World J 2014;7. http://dx.doi.org/10.1155/2014/802701
13. Jurjus AR, Khoury NN, Reimund JM. Animal models of inflammatory bowel disease. J Pharmacol Toxicol Methods 2004;50:81-92.
14. Cetinkaya A, Bulbuloglu E, Kantarceken B, Ciralik H, Kurutas EB, Buyukbese MA, et al. Effects of L-carnitine on oxidant/antioxidant status in acetic acid-induced colitis. Dig Dis Sci 2006;51:488-94.
15. Ghatule R, Shalini G, Gautam M, Singh A, Joshi V, Goel R. Effect of azadirachta indica leaves extract on acetic acid-induced colitis in rats: role of antioxidants, free radicals and myeloperoxidase. Asian Pacific J Tropical Disease 2012;2:S651-S7.
16. Mohammadi S, Najafi M, Hamzeiy H, Maleki Dizaji N, Pezeshkian M, Sadeghi Bazargani H, et al. Protective effects of methylsulfonylmethane on hemodynamics and oxidative stress in monocrotaline-induced pulmonary hypertensive rats. Adv Pharmacol Sci 2012. http://dx.doi.org/10.1155/2012/507278
17. Lee J, Lee HJ, Park JD, Lee SK, Lee SI, Lim HD, et al. Anti-cancer activity of highly purified sulfur in immortalized and malignant human oral keratinocytes. Toxicol In Vitro 2008;22:87-95.
18. Townsend DM, Tew KD, Tapiero H. Sulfur containing amino acids and human disease. Biomed Pharmacother 2004;58:47-55.
19. Métayer S, Seiliez I, Collin A, Duchene S, Mercier Y, Geraert PA, et al. Mechanisms through which sulfur amino acids control protein metabolism and oxidative status. J Nutr Biochem 2008;19:207-15.
20. Al laham S, Mansour G. Beneficial effects of MSM treatment on the development of experimental colitis induced by acetic acid. Int J Toxicol Pharmacol Res 2016;8:269-74.
Published
How to Cite
Issue
Section
