PREVALENCE AND ANTIBIOTIC SUSCEPTIBILITY PATTERN OF PSEUDOMONAS SPECIES ISOLATED FROM CLINICAL SAMPLES IN A TERTIARY CARE HOSPITAL

M. ABDUR RAHMAN; POOJA NAIR

doi:10.22159/ijcpr.2021v13i1.40814

Authors

M. ABDUR RAHMAN Department of Microbiology, Saveetha Medical College and Hospital, Thandalam, Chennai
POOJA NAIR Department of Microbiology, Saveetha Medical College and Hospital, Thandalam, Chennai

DOI:

https://doi.org/10.22159/ijcpr.2021v13i1.40814

Keywords:

Pseudomonas aeruginosa, Antimicrobial susceptibility, Multidrug resistance

Abstract

Objective: Pseudomonas aeruginosa is the commonest agent causing opportunistic nosocomial infections, contributing to mortality and morbidity worldwide. Along with its intrinsic and acquired resistance mechanisms, infections caused by Pseudomonas species further lead to treatment failure. This study was done to determine the prevalence and antimicrobial susceptibility pattern of different species of Pseudomonas isolated from various clinical samples by phenotypic methods.

Methods: This study involved the examination of clinical samples for various species of Pseudomonas by using different standard biochemical tests. Their antimicrobial susceptibility profile was performed by the Kirby Bauer disc diffusion method. Analysis of the antibiogram pattern was done to study the multi-drug resistance among Pseudomonas species.

Results: Out of the 1249 bacterial isolates, Pseudomonas species accounted for 12.9%. Pseudomonas species showed maximum resistance to gentamicin (31.4%), followed by ciprofloxacin (30.2%. and showed the least resistance tocolistin and polymyxin B. 46 isolates out of the 162 (28%) were found to be Multi-Drug Resistant (MDR) Pseudomonas aeruginosa.

Conclusion: The present study highlights that Pseudomonas species remains a major cause of hospital-acquired infections. Multidrug resistance was observed in most of the strains, which makes the therapeutic options more difficult. Surveillance of antimicrobial resistance and strict infection control measures are essentially to be practiced in managing and control of infections caused by Pseudomonas.

Downloads

Download data is not yet available.

References

1. Palleroni NJ. Introduction to the family pseudomonadaceae. In: Starr MP, Stolp H, Trüper HG, Balows A, Schlegel HG. (eds) The Prokaryotes. Springer, Berlin, Heidelberg; 1981.
2. Swaraj Mohanty, Bighneswar Baliyarsingh, Suraja Kumar Nayak. Antimicrobial resistance in Pseudomonas aeruginosa: a concise review; 2019. DOI:10.5772/intechopen.88706
3. Jaswinder Sharma, Surindersingh, Amarjitkaur Gill, Amandeepkaur. Prevalence and antimicrobial susceptibility pattern of Pseudomonas aeruginosa isolated from pus samples in a tertiary care Hospital, Bathinda. Int J Contemporary Med Res 2016;3:3481-3.
4. Temesgen Bekele, Amene Tesfaye, Hebtewold Deti Waktola. Pseudomonas aeruginosa isolates and their antimicrobial susceptibility pattern among catheterized patients at Jimma University Teaching Hospital, Jimma, Ethiopia. BMC Res Notes 2015;8:488.
5. Kiran A Deshmukh, Deepak Manthale. Prevalence and antibiotic susceptibility of Pseudomonas aeruginosa isolated from chronic suppurative otitis media. Int J Otorhinolaryngol Head Neck Surg 2017;3:56-60.
6. Josef Yayan, Beniam Ghebremedhin, Kurt Rasche. Antibiotic resistance of Pseudomonas aeruginosa in pneumonia at a single university hospital center in germany over a 10 Y Period. PLoS ONE 2015;10:e0139836.
7. Tarig MS Alnour, Eltayib Hassan Ahmed Abakur. Multidrug-resistant Pseudomonas (P) aeruginosa: medical impact, pathogenicity, resistance mechanisms and epidemiology. JSM Microbiol 2017;5:1046.
8. Alain C Juayang. Five-year antimicrobial susceptibility of Pseudomonas aeruginosa from a local tertiary hospital in Bacolod City, Philippines. Trop Med Infect Dis 2017;2:28.
9. Sumaira Qayoom, Afreen Rashid, Amrish Kohli, Talat Masoodi, Muzafar Amin. Prevalence and antibiotic sensitivity pattern of Pseudomonas aeruginosa isolate from respiratory samples, pus samples and body fluids in a tertiary care hospital, Kashmir. Indian J Microbiol Res 2019;6:345–9.