IN VITRO EVALUATION OF ANTHELMINTIC ACTIVITY OF AQUEOUS EXTRACT OF ARDISIA COLORATA ROXB. LEAVES IN ADULT EARTHWORMS

PRASENJIT SARKAR; N. MEENA DEVI; U. DHARMARAJ MEETEI; GOBINATH V.

doi:10.22159/ijcpr.2023v15i1.2063

Authors

PRASENJIT SARKAR Department of Pharmacology, Regional Institute of Medical Sciences, Imphal, Manipur, 795004
N. MEENA DEVI Department of Pharmacology, Regional Institute of Medical Sciences, Imphal, Manipur, 795004
U. DHARMARAJ MEETEI Department of Pharmacology, Regional Institute of Medical Sciences, Imphal, Manipur, 795004
GOBINATH V. Department of Pharmacology, Regional Institute of Medical Sciences, Imphal, Manipur, 795004

DOI:

https://doi.org/10.22159/ijcpr.2023v15i1.2063

Keywords:

Ardisia colorata roxb, Anthelmintic activity

Abstract

Objective: The study aims to evaluate the anthelmintic activity of aqueous extract of Ardisia colorata Roxb. leaves (AQEAC) using adult earthworms (Pheretima posthuman).

Methods: The total of 24 adult earthworms were divided into four groups, with six worms in each group (n=6). The anthelmintic activity of AQEAC at two different doses (25 mg/ml and 50 mg/ml) was evaluated by assessing the time of paralysis (min) and time of death (min) of the earthworms. Albendazole (25 mg/ml) was used as standard and 2% gum acacia as control.

Results: The result showed that AQEAChad significant anthelmintic activity (p<0.001) in a dose-dependent manner but was less potent than the standard drug albendazole.

Conclusion: AQEAC demonstrated significant anthelmintic activity but was less potent than the standard drug albendazole. However, further studies with higher doses are required to evaluate the dose-dependent activity and to evaluate the exact mechanism responsible for anthelmintic activity.

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References

Patel PR, Raval BP, Karanth HA, Patel VR. Potent antitumor activity of Rubia cordifolia. Int J Phytomed. 2010 Jan 1;2(1).

Datta S, Ningthoujam G, Zosangpuii C, Shyamasakhi P, Meena N. In vitro evaluation of anthelmintic activity of ethanolic extract of Centella asiatica Linn. In Indian adult earthworms. Int J Curr Pharm Sci. 2021 Sep;15:39-41. doi: 10.22159/ijcpr.2021v13i5.1884.

Roy SD, Goswami R, Das S, Shil D, Baniya R, Haldar S. Pharmacognostic evaluation and anthelmintic activity of leaf and stem extract of Carica papaya. J Pharm Res. 2012 Sep;5(9):4763-6.

KantiSinha S. In vitro evaluation of the anthelmintic activity of ethanolic extract of leaves of toona ciliata m. roem on Indian earthworm. IJAR 2019;7(7):250-3. doi: 10.21474/IJAR01/9352.

Sanjeev S, Murthy MK, Sunita Devi M, Khushboo M, Renthlei Z, Ibrahim KS. Isolation, characterization, and therapeutic activity of bergenin from marlberry (Ardisia colorata Roxb.) leaf on diabetic testicular complications in Wistar albino rats. Environ Sci Pollut Res Int. 2019 Mar;26(7):7082-101. doi: 10.1007/s11356-019-04139-9, PMID 30648235.

Sumino M, Sekine T, Ruangrungsi N, Ikegami F. Ardisiphenols A-C, novel antioxidants from the fruits of Ardisia colorata. Chem Pharm Bull (Tokyo). 2001;49(12):1664-5. doi: 10.1248/cpb.49.1664, PMID 11767097.

Meshram GG, Kumar A, Rizvi W, Tripathi CD, Khan RA. Evaluation of the anti-inflammatory activity of the aqueous and ethanolic extracts of the leaves of Albizzia lebbeck in rats. J Tradit Complement Med. 2016 Apr 1;6(2):172-5. doi: 10.1016/j.jtcme.2014.11.038, PMID 27114941.

Ajaiyeoba EO, Onocha PA, Olarenwaju OT. In vitro anthelmintic properties of Buchholzia coriaceae and gynandropsis gynandra extracts. Pharm Biol. 2001 Jan 1;39(3):217-20. doi: 10.1076/phbi.39.3.217.5936.