ISOLATION OF MULTI DRUG RESISTANT BACTERIA FROM BORE WATER SAMPLES IN AND AROUND VISAKHAPATNAM

TIRUVAIPATI SIRISHA; SWAPNA SASAPU; PAYALA VIJAYALAKSHMI; PILLI HEMA PRAKASH KUMARI

doi:10.22159/ijcpr.2023v15i2.2083

Authors

TIRUVAIPATI SIRISHA Department of Microbiology, Government Medical College, Srikakulam Dist, Andhra Pradesh, India
SWAPNA SASAPU Department of Microbiology, Andhra Medical College, Visakhapatnam
PAYALA VIJAYALAKSHMI Department of Microbiology, GITAM Institute of Medical Sciences and Research, GITAM (Deemed to be University), Visakhapatnam 530045, Andhra Pradesh, India
PILLI HEMA PRAKASH KUMARI Department of Microbiology, GITAM Institute of Medical Sciences and Research, GITAM (Deemed to be University), Visakhapatnam 530045, Andhra Pradesh, India

DOI:

https://doi.org/10.22159/ijcpr.2023v15i2.2083

Keywords:

MDR microorganisms, MPN, Water-borne diseases, Antibiotics

Abstract

Objective: The aim of the present study was to determine the levels of groundwater contamination by isolating total coliforms, fecal coliforms, and bacterial pathogens.

Methods: A total of 32 water samples, comprising 5 samples from each GVMC urban area zone, Visakhapatnam were randomly collected from different areas. Evaluation of Total and fecal coliform counts was done by using multiple-tube fermentation technique and were reported as the most probable number (MPN) of organisms present per 100 ml of sample.

Results: The samples collected from the zones Yendada (zone I), Marripalem (zone IV), Chinna musidiwada (zone VI) showed the highest total and fecal coliform counts (MPN>180). Nearly 30 samples (94%) yielded bacterial isolates. Among the isolates Pseudomonas spp. showed highest susceptibility to cefepime and imipenem antibiotics. All the microorganisms isolated were multidrug resistant with a multidrug antibiotic resistant index more than 0.2.

Conclusion: These multidrug-resistant (MDR) organisms are considered as a major threat to the public health and should receive more attention.

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