QUANTITATIVE ANALYSIS OF CYTISINE IN THERMOPSIS ALTERNIFLORAE USING HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY

Authors

  • KRUPA GADHVI Department of Pharmacognosy and Phytochemistry, L. M. College of Pharmacy, Ahmedabad 380009, Gujarat, India
  • KARUNA MODI Department of Pharmacognosy and Phytochemistry, L. M. College of Pharmacy, Ahmedabad 380009, Gujarat, India
  • MAMTA SHAH Department of Pharmacognosy and Phytochemistry, L. M. College of Pharmacy, Ahmedabad 380009, Gujarat, India

DOI:

https://doi.org/10.22159/ijcpr.2023v15i2.2087

Keywords:

Thermopsis alterniflorae, Cytisine, High-performance thin-layer chromatography

Abstract

Objective: An optimized high-performance thin-layer chromatography (HPTLC) method has been established for the quantification of cytisine in Thermopsis alterniflorae Regel and Schmalh.

Methods: Alcoholic extract of the aerial parts were prepared using Soxhlet extraction method. Separation was achieved on silica gel 60 F254 HPTLC plates using toluene-ethyl acetate-diethyl amine (7:2:1, v/v) as the mobile phase. The quantitation of cytisine was carried out using densitometric scanning at 545 nm after derivatization using Dragendorff’s reagent.

Results: The linear regression analysis data for the calibration plot showed a good linear relationship (r2 = 0.9849) in the concentration range 10 to 15 µg/spot. The method was validated for precision, repeatability, accuracy, specificity, limit of detection and limit of quantification. The average recovery was 99.0899% indicating good accuracy. The percentage yield of cytisine obtained was 0.5075+0.0135 % w/w.

Conclusion: The proposed HPTLC method was found to be simple, sensitive, accurate, reproducible, and robust.

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References

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Published

15-03-2023

How to Cite

GADHVI, K., K. MODI, and M. SHAH. “QUANTITATIVE ANALYSIS OF CYTISINE IN THERMOPSIS ALTERNIFLORAE USING HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY”. International Journal of Current Pharmaceutical Research, vol. 15, no. 2, Mar. 2023, pp. 33-35, doi:10.22159/ijcpr.2023v15i2.2087.

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