QUANTITATIVE ANALYSIS OF CYTISINE IN THERMOPSIS ALTERNIFLORAE USING HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY

KRUPA GADHVI; KARUNA MODI; MAMTA SHAH

doi:10.22159/ijcpr.2023v15i2.2087

Authors

KRUPA GADHVI Department of Pharmacognosy and Phytochemistry, L. M. College of Pharmacy, Ahmedabad 380009, Gujarat, India
KARUNA MODI Department of Pharmacognosy and Phytochemistry, L. M. College of Pharmacy, Ahmedabad 380009, Gujarat, India
MAMTA SHAH Department of Pharmacognosy and Phytochemistry, L. M. College of Pharmacy, Ahmedabad 380009, Gujarat, India

DOI:

https://doi.org/10.22159/ijcpr.2023v15i2.2087

Keywords:

Thermopsis alterniflorae, Cytisine, High-performance thin-layer chromatography

Abstract

Objective: An optimized high-performance thin-layer chromatography (HPTLC) method has been established for the quantification of cytisine in Thermopsis alterniflorae Regel and Schmalh.

Methods: Alcoholic extract of the aerial parts were prepared using Soxhlet extraction method. Separation was achieved on silica gel 60 F₂₅₄ HPTLC plates using toluene-ethyl acetate-diethyl amine (7:2:1, v/v) as the mobile phase. The quantitation of cytisine was carried out using densitometric scanning at 545 nm after derivatization using Dragendorff’s reagent.

Results: The linear regression analysis data for the calibration plot showed a good linear relationship (r² = 0.9849) in the concentration range 10 to 15 µg/spot. The method was validated for precision, repeatability, accuracy, specificity, limit of detection and limit of quantification. The average recovery was 99.0899% indicating good accuracy. The percentage yield of cytisine obtained was 0.5075+0.0135 % w/w.

Conclusion: The proposed HPTLC method was found to be simple, sensitive, accurate, reproducible, and robust.

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