PHYTOCHEMICALS SCREENING, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITY OF CARICA PAPAYA LEAF EXTRACTS
DOI:
https://doi.org/10.22159/ijcpr.2024v16i3.4087Keywords:
Carica papaya, Phytochemicals, Extraction, Antioxidant, AntibacterialAbstract
Objective: The objective of the present study aimed at investigating the phytochemical antioxidant and anti-microbial properties of Carica papaya leaf extracts.
Methods: As phytochemicals are biologically active compounds and a powerful group of plant chemicals believed to stimulate the immune system along with antioxidants, the molecules which hinder oxidation of other molecules by the process of inhibiting or by generating the oxidizing chain reactions and preventing diseases. The anti-microbial activity on various gram-positive and gram-negative bacteria were determined using zone of inhibition and antioxidant by the 2,2,1-diphenyl-1-picrylhydrazyl method.
Results: Phytochemical screening, antioxidant and antibacterial potential were determined using different aqueous and organic solvents in addition to the determination of trace element in leaves of C. papaya. Antioxidant activity using DPPH free radical scavenging assay indicated leaves extracts leaves showed inhibition of per oxidation. The result showed that the ethanolic extract of C. papaya possessed a significant antioxidant activity as compared to methanol and aqueous extract. The antibacterial study showed leaves extract is the best to cope infectious action of bacteria.
Conclusion: This study was conducted to test the medicinal profile of C. papaya by extracting secondary metabolites with organic and aqueous solvents. Ethanol was found to be the best solvents of choice to extract natural products to get maximum medicinal benefits and could be used to medicinal formulation against different infectious diseases.
Downloads
References
Agar OT, Dikmen M, Ozturk N, Yilmaz MA, Temel H, Turkmenoglu FP. Comparative studies on phenolic composition, antioxidant, wound healing and cytotoxic activities of selected Achillea L. species growing in turkey. Molecules. 2015;20(10):17976-8000. doi: 10.3390/molecules201017976, PMID 26437391.
Maneesh K, Vijayabhaskar K, Firdouse H, Srinivasa Rao P, Prajwitha M, Swetha S. Evaluation of antimicrobial of P. vesicularis, streptococcus faecalis, aeromonas hydrophilia, salmonela typhae, stphylococcus cohni, serratia ficaria and E. coli. of crude and n-butanol fraction fruit latex of Carica papaya L. (Caricaceae). Asian J Pharm Res. 2021;11(2):92-4. doi: 10.52711/2231-5691.2021.00017.
Anand David AV, Arulmoli R, Parasuraman S. Overviews of biological importance of quercetin: a bioactive flavonoid. Pharmacogn Rev. 2016;10(20):84-9. doi: 10.4103/0973-7847.194044, PMID 28082789.
Asghar N, Naqvi SA, Hussain Z, Rasool N, Khan ZA, Shahzad SA. Compositional difference in antioxidant and antibacterial activity of all parts of the Carica papaya using different solvents. Chem Cent J. 2016;10(5):5. doi: 10.1186/s13065-016-0149-0, PMID 26848308.
Canini A, Alesiani D, D’Arcangelo G, Tagliatesta P. Gas chromatography-mass spectrometry analysis of phenolic compounds from Carica papaya L. leaf. J Food Compos Anal. 2007;20(7):584-90. doi: 10.1016/j.jfca.2007.03.009.
Purohit P, Gaur H. Lipid based nanoparticles in cancer therapy. Indo Am J Pharm Res. 2023;4:225-29.
Tejaram RS, Purohit P, Sanju KR. Formulation and evaluation of cefixime-loaded grafted gellan gum tablet. Int J Pharm Biol Sci. 2023;13(3):89-98.
Purohit P, Sahil. A review on wound healing activity of medicinal plants. International Journal of Pharmaceutics and Drug Analysis. 2018;6(7):545-7.
Ismail HF, Hashim Z, Soon WT, Rahman NS, Zainudin AN, Majid FA. Comparative study of herbal plants on the phenolic and flavonoid content, antioxidant activities and toxicity on cells and zebrafish embryo. J Tradit Complement Med. 2017;7(4):452-65. doi: 10.1016/j.jtcme.2016.12.006, PMID 29034193.
Kapoor M, Howard R, Hall I, Appleton I. Effects of epicatechin gallate on wound healing and scar formation in a full thickness incisional wound healing model in rats. Am J Pathol. 2004;165(1):299-307. doi: 10.1016/S0002-9440(10)63297-X, PMID 15215184.
Kurahashi T, Fujii J. Roles of antioxidative enzymes in wound healing. J Dev Biol. 2015;3(2):57-70. doi: 10.3390/jdb3020057.
Lodhi S, Singhai AK. Wound healing effect of flavonoid-rich fraction and luteolin isolated from Martynia annua Linn. on streptozotocin-induced diabetic rats. Asian Pac J Trop Med. 2013;6(4):253-9. doi: 10.1016/S1995-7645(13)60053-X, PMID 23608325.
Shelke M, Tamboli A, Sonawane P, Sadaphal P, Mankar SD. A review on pharmacognosy and pharmacological activity of carica papaya leaf. Res J Pharmacogn Phytochem. 2021;13(4):200-4. doi: 10.52711/0975-4385.2021.00035.
Lydia E, John S, Mohammed R, Sivapriya T. Investigation on the phytochemicals present in the fruit peel of Carica papaya and evaluation of its antioxidant and antimicrobial property. Res J Pharmacogn Phytochem. 2016;8(4):217-22. doi: 10.5958/0975-4385.2016.00032.7.
Amutha K, Arokia Doss DV. In vitro antioxidant activity of ethanolic extract of barleria cristata L. leaves. Res J Pharmacogn Phytochem. 2009;1(3):209-12.
Potbhare M, Khobragade D. In vitro evaluation of the antioxidant potential of ayurvedic preparations lauha bhasma and mandura bhasma. Asian Journal Pharmaceutical Research. 2017;7(2):63-6. doi: 10.5958/2231-5691.2017.00011.9.
Madhu, Swapna, Neelima, Monic V, Shah A. Comparative evaluation of the antioxidant activity of some medicinal plants popularly used in India. Asian J Res Pharm Sci. 2012;2(3):98-100.
Jadhav GB, Saudagar RB. Free radical scavenging and antioxidant activity of punica granatum linn. Asian J Res Pharm Sci. 2014;4(2):51-4.
Selvakumar K, Madhan R, Srinivasan G, Baskar V. Antioxidant assays in pharmacological research. Asian J Pharm Technol. 2011;1(4):99-103.
Vidya M, Bhavya SV, Sunitha PS, Rashmi P. Benefits of papaya. Inter J of Adva in Nur Manag 2019;7(2). doi: 10.5958/2454-2652.2019.00040.4.
Kanagavalli M, Anuradha R. A study on phytochemical constituents and in vitro antioxidant activity of Carica papaya. Res J Pharm Technol. 2012 Jan;5(1):119-20.
Konduri MK, Uppuluri KB, Chintha R, Mulla S, Peruri R. In vitro antimicrobial activity of four indigenous medicinal plants belonging to Bapatla, A. P. Res J Pharm Technol. 2010;3(2):461-5.
Azam W, Rai V, Jawed R, Jaywardhane K, Thakur V, Jain V. Comparison of antifungal and antimicrobial activities in different parts of Carica papaya linn. Asian J Pharm Res. 2023;13(4):213-8. doi: 10.52711/2231-5691.2023.00040.
Published
How to Cite
Issue
Section
Copyright (c) 2024 PRASHANT PUROHIT, MAHESH KUMAR KATARIA
This work is licensed under a Creative Commons Attribution 4.0 International License.
