MICROBICIDAL AND CYTOTOXIC ACTIONS OF METHANOLIC CRUDE EXTRACTS OF A. MULTIFLORA.-ROXB

Authors

  • S. A. Mobarak Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh
  • Shafayat Hossain Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh
  • Sagir Mia Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh

Keywords:

Microbicidal, Ammannia multiflora, Inhibition and Cytotoxic

Abstract

Objective: The study presented here was carried out for the evaluation of microbicidal and cytotoxic potential of methanolic extract of leaves of Ammannia multiflora. The microbicidal activity was tested against 16 bacteria. The cytotoxic activity was tested against brine shrimp species Artemia salina.

Methods: The microbicidal effect of the plant extracts was tested in vitro by the disc diffusion method with 16 standard bacterial strains. Artemia salina; a brine shrimp species; was used to assess cytotoxic activity.

Results: Among the subjected 16 different bacteria; in case of S. boydii; the zone of inhibition was higher (22.0 mm) at 500 µg/ml concentration. Whereas in the case of E. foecalis, the zone of inhibition was higher (8.0 mm) at 250 µg/ml concentration. In S. pyogenes; zone of inhibition was higher at 500 µg/ml concentration (13.0 mm). The growth of S. saprophyticus (9 mm), S. agalactiae (7 mm) and S. sonnei (7 mm) was also moderately inhibited. The extract exhibited effectiveness against 7 different species of bacteria out of 16. Cytotoxic effect was determined in lethality bioassay of brine shrimp species Artemia salina. The plant extracts exhibited significant cytotoxic property which was reflected in LC50 and LC90values of 20.42µg/ml and 229.09 µg/ml respectively. Cytotoxic property was found as dose dependent manner.

Conclusion: These results suggest that, Ammania multiflora can effectively be used for its significant microbicidal action. It is also effective as cytotoxic agent and thus, could justify its use in traditional medicine.

Downloads

Download data is not yet available.

References

Craig WJ. Health promoting properties of common herbs. Am J Clin Nutr 1999;70:491-9.

Cook NC, Samman S. Flavonoids-Chemistry, metabolism, cardioprotective effects, and dietary sources. J Nutr Biochem 1996;7:66-76.

Manandhar NP. Plants and People of Nepal. Timber Press: USA; 2000. p. 50.

Doughari JH, El-mahmood AM, Tyoyina I. Antimicrobial activity of leaf extracts ofSenna obtusifolia (L). Afr J Pharm Pharmacol 2008;2:7–13.

Khan AV, Ahmed QU, Shukla I, Khan AA. Antibacterial activity of leaves extracts ofTrifolium alexandrinum Linn. against pathogenic bacteria causing tropical diseases. Asian Pac J Trop Biomed 2012;2:189–94.

Arcanjo DDR, Albuquerque ACM, Melo-Neto B, Santana LCLR, Medeiros MGF, Citó AMGL. Bioactivity evaluation against Artemia salina Leach of medicinal plants used in Brazilian North-eastern folk medicine. Braz J Biol 2012;72:505–9.

Kirtikar KR, Basu BD. Editors. Indian Medicinal plants. vol. 1. 2nd edition; 1999. p. 312-3.

Haining Qin, Shirley A. Graham & Michael G. Gilbert. Flora of China.†Vol. 13. 2014. p. 274, 290, 400.

Switu Jani, Vinay J Shukla, CR Harisha. Comparative pharmacognostical and phytochemical study on Bergenia ligulata Wall and Ammania buccifera Linn; Ayu 2013;34(4):406–10.

Ghani A. Medicinal Plants of Bangladesh†Chemical constituents and Uses, Asiatic Society of Bangladesh, Dhaka; 1998. p. 8-10.

Mayers; Hytochemical methods(a guide to modern techniques to plant analysis). 3rd ed. Champan and Hall; 1982. p. 335-7.

Bauer AW, Kirby WMM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standard single disc method. Am J Clin Pathol 1966;45:493-6.

McLaughlin JL, Rogers LL. The use of biological assays to evaluate botanicals. Drug Inf 1998;32:513-24.

Meyer BN, Ferringni NR, Puam JE, Lacobsen LB, Nichols DE, McLaughlin JL. Brine shrimp: a convenient general bioassay for active constituents. Planta Med 1982;45:31-2.

Published

10-04-2015

How to Cite

Mobarak, S. A., S. Hossain, and S. Mia. “MICROBICIDAL AND CYTOTOXIC ACTIONS OF METHANOLIC CRUDE EXTRACTS OF A. MULTIFLORA.-ROXB”. International Journal of Current Pharmaceutical Research, vol. 7, no. 2, Apr. 2015, pp. 54-56, https://mail.innovareacademics.in/journals/index.php/ijcpr/article/view/6293.

Issue

Section

Original Article(s)