NEPHROPROTECTIVE EFFECT OF LYCOPENE IN HYPERGLYCEMIA INDUCED OXIDATIVE STRESS IN MALE WISTAR RATS
Keywords:
Male wistar rats oxidative stress, Lycopene, Diabetes, Kidney diseaseAbstract
Objective: Diabetes mellitus is the chronic metabolic disorder characterized by chronic hyperglycemia associated with absolute or relative deficiency in insulin secretion or insulin action. The most commonly accepted cause of diabetes is the oxidative damage that is caused by free radicals generation. Free radicals have high ability to attract electrons from macromolecules such as carbohydrates, protein, lipid and DNA. Excessive Reactive oxygen species (ROS) can cause structural deterioration and instability of the macromolecules, consequently affecting proper cellular signaling pathways, gene regulation and function. The present study was conducted to investigate the nephroprotective effect of lycopene in alloxan induced type I diabetes.
Methods: Male wistar rats were divided in to 5 groups 6 in each. Group1 as control, Group II. III,IV and V were diabetic groups. Group II diabetic control, Group III treated with protamine zinc insulin 0.9 u/100 gm s. c. Group IV and V treated with 2.5 mg/kg and 5 mg/kg of lycopene. After 3 weeks blood samples were collected from all the groups of animals to measure Lipid peroxidation. Serum glucose, urea and creatinine.
Results: The serum Glucose, urea and creatinine were significantly increased in untreated diabetic rats. In addition, there was significant rise in lipid peroxidation.
Conclusion: In this study, oxidative damage with diabetes was ameliorated with administration of lycopene. The results of this study indicate that lycopene is an effective nutritional component to alleviate or prevent the complications.
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References
Duckworth WC. Hyperglycemia and cardiovascular disease. Curr Atheroscler 2001;3:383–91.
Varzi HN, Esmailzadeh S, Morovvati H, Avizeh R, Shahriari A, Givi ME. Effect of Silymarin and vitamine E on gentamicin-induced nephrotoxicity in dogs. J Vet Pharmacol Ther 2007;30:477-87.
DCCT. The diabetes control and complications trial research group. The effect of intensive treatment of diabetes on the development and progression of long-term complications in insulin-dependent diabetes mellitus. N Engl J Med 1993;329:977–86.
Rice EC, Miller N, Paganaga G. Antioxidant properties of phenolic compounds. Trends Plant Sci 1997;2:152–9.
Valko M, Leibfritz D, Moncol J, Cronin MTD, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 2007;39:44–84.
Haskins K, Bradley B, Powers K. Oxidative stress in type 1 diabetes. Ann N Y Acad Sci 2003;1005:43–54.
Kowluru RA, Chan PS. Oxidative stress and diabetic retinopathy. Exp Diabetes Res 2007;4:43603.
The expert committee on the diagnosis and classification of diabetes mellitus: Report of the expert committee on the diagnosis and classification of diabetes mellitus. Diabetes Care 1997;20:1183–97.
Anurag kuhad, Richa S, Dethi, Kanwaljit chopra. Lycopene attenuates diabetes associated cognitive decline in rats. Life Sci 2008;83:128-34.
American diabetes association standards of medical care in diabetes (position statement). Diabetes Care 2004;27(Suppl 1):S15-S35.
The diabetes control and complications trial research group: The effect of intensive treatment of diabetes on the development and progression of long-term complications in insulin-dependent diabetes mellitus. N Engl J Med 1993;329(14):977-86.
Giugliano D, Ceriello A, Paolisso G. Oxidative stress and diabetic vascular complications. Diabetes Care 1996;19(3):257-67.
Ceriello A. New insights on oxidative stress and diabetic complications may lead to a "causal" antioxidant therapy. Diabetes Care 2003;26(5):1589-96.
Ceriello A, Motz E. Is oxidative stress the pathogenic mechanism underlying insulin resistance, diabetes, and cardiovascular disease? The common soil hypothesis revisited. Arterioscler Thromb Vasc Biol 2004;24(5):816-23.
Turko IV, Marcondes S, Murad F. Diabetes-associated nitration of tyrosine and inactivation of succinyl-CoA: 3-oxoacid CoA-transferase. Am J Physiol: Heart Circ Physiol 2001;281(6):H2289-94.
Cuzik TJ, West NE, Black E, McDonald D, Ratnatunga C, Pillai R, Channon KM. Vascular superoxide production by NAD(P)H oxidase: association with endothelial dysfunction and clinical risk factors. Circ Res 2000;86(9):E85-90.
Cuzik TJ, Mussa S, Gastaldi D, Sadowski J, Ratnatunga C, Pillai R, et al. Mechanisms of increased vascular superoxide production in human diabetes mellitus: role of NAD(P)H oxidase and endothelial nitric oxide synthase. Circulation 2002;105(14):1656-62.
Aliciguzel Y, Ozen I, Aslan M, Karayalcin U. Activities of xanthine oxireductase and antioxidant enzymes in different tissues of diabetic rats. J Lab Clin Med 2003;142(3):172-7.
Haskins K, Bradley B, Powers K. Oxidative stress in type 1 diabetes. Ann N Y Acad Sci 2003;1005:43–54.
Kowluru RA, Chan PS. Oxidative stress and diabetic retinopathy. Exp Diabetes Res 2007;4:43603.