VALIDATED SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF CHLORAMPHENICOL IN PURE AND IN ITS DOSAGE FORM

Authors

  • P. Suguna Department of Chemistry, S. V. University, Tirupati 517502, A. P., India
  • B. Sathyanarayana
  • N. V. S. Naidu

Abstract

A simple, précis, rapid sensitive and accurate spectrophotometric methods have been developed for the estimation of Chloramphenicol UV in pure form and its pharmaceutical formulations based on oxidative coupling reaction UV with MBTH reagent at P H-4 which is extractable at 620 nm. Beer's law is obeyed in the concentration range 1-6 ml (10-60 µgml-1). The developed method was applied directly and easily for the analysis of the pharmaceutical formulations. RSD was found to be 0.0194 % and recovery 99.73%. The method was completely validated and proven to be rugged. The interferences of the ingredients and excipients were not observed. The repeatability and the performance of the proved method were established by point and internal hypothesis and through recovery studies.

Keywords: Spectrophotometry, Chloramphenicol, MBTH, Oxidative coupling.

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References

Database of National Toxicology Program (NTP), U. S. Department of Health and Human Services, Substance Profiles Report on Carcinogens, 11th ed., http://ntp. niehs. nih. gov/ntp/roc/eleventh/profiles/s032chlo. pdf.

P. A. Guya, D. Royerb, P. Mottiera, E. Gremauda, A. Perisseta, and R. H. Stadlerc, J. Chromatogr., A, 2004, 1054, 365.

S. Impens, W. Reybroeck, J. Vercammen, D. Courtheyn, S. Ooghe, K. De Wasch, W. Smedts, and H. De Brabander, Anal. Chim. Acta, 2003, 483, 153.

L. Agüí, A. Guzmán, P. Yáñez-Sedeño, and J. M. Pingarrón, Anal. Chim. Acta, 2002, 461, 65.

P. Viñas, N. Balsalobre, and M. Hernández-Córdoba, Anal Chim. Acta, 2006, 558, 11.

R. J. Shakila, S. A. Vyla, R. S. Kumar, G. Jeyasekaran, and G. I. Jasmine, Food Microbiology, 2006, 23, 47.

P. Mottier, V. Parisod, E. Gremaud, P. A. Guy, and R. H. Stadler, J. Chromatogr., A, 2003, 994, 75.

M. Ramos, P. Muñoz, A. Aranda, I. Rodriguez, R. Diaz, and J. Blanca, J Chromatogr., B, 2003, 791, 31.

J. Storey, A. Pfenning, S. Turnipseed, G. Nandrea, R. Lee, C. Burns, and M. Madson, Lab. Inform. Bull., 2003, 4306.

B. K. Neuhaus, J. A. Hurlbut, and W. Hammack, Lab. Inform. Bull., 2002, 4290.

Pfenning, S. Turnipseed, J. Roybal, C. Burns, M. Madson, J. Storey, and R. Lee, Laboratory Information Bulletin, 2002, 4284.

H. Y. Shen and H. L. Jiang, Anal. Chim. Acta, 2005, 535, 33.

G. Scortichini, L. Annunziata, M. N. Haouet, F. Benedetti, I. Krusteva, and R. Galarini, Anal. Chim. Acta, 2005, 535, 43

Gantverg, E. Shishani, and M. Hoffmana, Anal. Chim. Acta, 2003, 483, 125.

M. J. Bogusz, H. Hassan, E. Al-Enazi, Z. Ibrahim, and M. Al-Tufail, J Chromatogr., B, 2004, 807, 343.

W. Jin, X. Ye, D. Yu, and Q. Dong, J. Chromatogr., B, 2000, 741, 155.

B. Wang, L. Zhang, and Y. Z. Fang, Anal. Chim. Acta, 1999, 394, 309.

M. S. Collado, V. E. Mantovani, H. C. Goicoechea, and A. C. Olivieri, Talanta, 2000, 52, 909.

C. J. Eboka, J. Smart, and S. A. Adelusi, Tropical J. Pharm. Res., 2003, 2, 215.

C. A. Lindino and L. O. S. Bulhões, J. Braz. Chem. Soc., 2004, 15, 178.

M. C. Icardo, M. Misiewicz, A. Ciucu, J. V. Mateo, and J. M. Calatayud, Talanta, 2003, 60, 405.

V. David, R. M. Saèz, J. V. Mateo, and J. M. Calatayud, Analyst, 2000, 125, 1313.

S. Jolley, M. D. Koppang, T. M. Jackson, and G. M. Swain, Anal. Chem., 1997, 69, 4099.

T. Yano, D. A. Tryk, K. Hashimoto, and A. Fujishima, J Electroanal. Chem., 1998, 145, 1870.

G. M. Swain and R. Ramesham, Anal. Chem., 1993, 65, 345.

J. W. Strojek, M. C. Granger, G. M. Swain, T. Dallas, and M. V. Holtz, Anal. Chem., 1996, 68, 2031.

E. Popa, H. Notsu, T. Miwa, D. A. Tryk, and A. Fujishima, Electrochem. Solid-State Lett. 1999, 2, 49.

J. S. Xu, Q. Y. Chen, and G. M. Swain, Anal. Chem., 1998, 70, 3146.

B. V. Sarada, T. N. Rao, D. A. Tryk, and A. Fujishima, Anal. Chem., 2000, 72, 1632.

W. Siangproh, N. Wangfuengkanagul, and O. Chailapakul, Anal. Chim. Acta, 2003, 499, 183.

T. N. Rao, I. Yagi, T. Miwa, D. A. Tryk, and A. Fujishima, Anal. Chem., 1999, 71, 2506.

N. Wangfuengkanagul and O. Chailapakul, J. Pharm. Biomed. Anal., 2002, 28, 841.

N. Wangfuengkanagul and O. Chailapakul, Talanta, 2002, 58, 1213.

W. Siangproh, P. Ngamukot, and O. Chailapakul, Sens. Actuators, B, 2003, 91, 60.

K. Boonsong, S. Chuanuwatanakul, N. Wangfuengkanagul, and O. Chailapakul, Sens. Actuators, B, 2005, 108, 627.

Preechaworapun, S. Chuanuwatanakul, Y. Einaga, K. Grudpan, S. Motomizu, and O. Chailapakul, Talanta, 2006, 68, 1726.

P. Ngamukot, T. Charoenraks, O. Chailapakul, S. Motomizu, and S. Chuanuwatanakul, Anal. Sci., 2006, 22, 111.

D. A. Skoog, D. M. West, F. J. Holler, and S. R. Crouch, Fundamentals of Analytical Chemistryâ€, 8th ed., 2004, Thomson Learning, Belmont, 694.

M. D. Corbett and B. R. Chipko, Antimicrob. AgentsChemother. 1978, 13, 193.

Chatwal GR, Anand SKJ. Instrumental Methods of Chemical Analysis, Himalaya Publishing House, Mumbai, 2003:2.108-2.109

Harris, D. C. (2003); Quantitative Chemical Analysis 6th ed.â€; 258-261, 407-422, first fig. @ pp. 453, 461-476, 707-709.

Chilukuri S. P. Sastry, Kolli Rama Rao. Determination of Cefadroxil by three simple spectrophotometric methods using oxidative coupling reaction. Mkrochin Acta 126, 167-172 (2003).

Published

10-04-2015

How to Cite

Suguna, P., B. Sathyanarayana, and N. V. S. Naidu. “VALIDATED SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF CHLORAMPHENICOL IN PURE AND IN ITS DOSAGE FORM”. International Journal of Current Pharmaceutical Research, vol. 7, no. 2, Apr. 2015, https://mail.innovareacademics.in/journals/index.php/ijcpr/article/view/6301.

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