VALIDATED SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF CHLORAMPHENICOL IN PURE AND IN ITS DOSAGE FORM
Keywords:
Spectrophotometry, Chloramphenicol, MBTH, Oxidative couplingAbstract
Objective: A simple, economic, selective, precise, and accurate UV-Visible spectrophotometric method for the analysis of Chloramphenicol in bulk drug and pharmaceutical formulations was developed and validated in the present study.
Methods: Based on oxidative coupling reaction with MBTH reagent at PH-4.5 which is extractable at 620 nm. The Beer's law is obeyed in the concentration range 1-6 ml (10-60 µg ml-1).
Results: The RSD was found to be 0.0194% and recovery is 99.73%. The method was completely validated and proven to be rugged. The interferences of the ingredients and recipients were not observed. The repeatability and the performance of the proved method were established by point and internal hypothesis and through recovery studies.
Conclusion: The method was found to be accurate and precise, as indicated by recovery studies close to 100 and % RSD is not more than 2. The summery of validation parameters of proposed UV-Visible method is given.
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References
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