CYTOTOXIC ACTIVITY OF ENDOPHYTIC FUNGI HHPCYL03 ISOLATED FROM CYMBOPOGON FLEXUOSUS NEES EX STEUD

Authors

  • Avinash Ks Department of PG Studies and Research in Applied Botany Kuvempu University, Shankaraghatta 577451, Shivamogga, Karnataka, India
  • Krishnamurthy Y. L. Department of PG Studies and Research in Applied Botany Kuvempu University, Shankaraghatta 577451, Shivamogga, Karnataka, India

Keywords:

Endophytic fungi, Anticancer, Inhibition, Metabolite

Abstract

Objective: Cymbopogon grass is one of common aromatic grass species used for extraction of essential oil. The endophytic fungus HHPCYL03isolated from the Cymbopogon flexuosus, a medicinal grass species collected from Kemmannugundi regions of Karnataka.

Methods: Secondary metabolites were extracted from fungi using organic solvent Ethyl Acetate and screened for anticancer assay against breast cancer cell (MDA-MB-231), lung cancer cell (Calu-6) and colorectal cancer cell (HCT116) lines.

Results: The extract showed the positive result against HCT116 cells.

Conclusion: The fungal endophytes certainly become a repository of good economically, socially benefitted bioactive compounds.

Downloads

Download data is not yet available.

References

Shadab Q, Hanif M, Chaudhury FM. Antifungal activity by lemon grass essential oils. Pak J Sci Ind Res 1992;35:246-59.

Dudai N, Weinstein Y, Krup M, Rabisnki T, Ofir R. Citral is a new inducer of caspase-3 in tumour cell lines. Planta Med 2005;71(5):484-8.

Bladt TT, Frisvad JC, Knudsen PB, Larsen TO. Anticancer and antifungal compounds from Aspergillus, Penicillium and other filamentous fungi. Molecules 2013;18:11338-76.

Turbyville TJ, Whitesell L, Wijeratne EM, Gunatilaka AA. The anticancer activity of the fungal metabolite terrecyclic acid A is associated with modulation of multiple cellular stress response pathways. Mol Cancer Ther 2005;4(10):1569-76.

Wiyakrutta S, Sriubolmas N, Panphut W, Thongon N, Danwiset-kanjana K, Ruangrungsi N, et al. Endophytic fungi with anti-microbial, anti-cancer and anti-malarial activities isolated from Thai medicinal plants. World J Microb Biot 2004;20:265-72.

Bacon CW, White JF. Biotechnology of Endophytic Fungi of Grasses, CRC, Boca Ratan, USA; 1994.

Saikkonen K, Faeth SH, Helander M, Sullivan TJ. Fungal endophytes: A continuum of interactions with host plants. Annu Rev Ecol Syst 1998;29:319-43.

Carroll GC. The biology of endophytism in plants with particular reference to woody plants. Microbiology of the Phyllosphere Ed. N Fokkema, J van den Heuvel’ Cambridge University Press: London; 1986. p. 205-22.

Azevedo JL, Maccheroni JW, Pereira JO, Araújo WL. Endophytic microorganisms: a review on insect control and recent advances on tropical plants. Electron J Biotechnol 2000;3(1):40-65.

Carroll, Carroll. Studies on incidence of coniferous needle endophytes in the pacific northwest. Can J Bot 1978;56:3034-43.

Strobel GA. Endophytes as sources of bioactive products. Microb Infect 2003;5:535–44.

Schardl CL, Leuchtmann A, Spiering MJ. Symbioses of grasses with seed borne fungal endophytes. Annu Rev Plant Biol 2004;55:315–40.

Stierle A, Strobel GA, Stierle D. Taxol and taxane production by Taxomycesandreanae. Science 1993;260:214-6.

Barnett HL, Hunter BB. Illustrated genera of imperfect fungi. 4th edition. APS Press: St. Paul, MN; 1998.

Ellis MB. More Dematiaceous Hyphomycetes. Common wealth Mycological Institute, Kew, Surrey, England; 1976.

Periyasamy G, Verekar SA, Khanna A, Mishra PD, Deshmukh SK. Anticancer activity of sclerotiorin, isolated from an endophytic fungus CephalothecafaveolataYaguchi, Nishim. and Udagawa. Indian J Exp Biol 2012;50:464-8.

Ruma K, Kumar S, Prakash HS. Antioxident, anti-inflammatory, antimicrobial and cytotoxic properties of fungal endophytes from Garcinia species. Int J Pharm Pharm Sci 2013;5(3):889-97.

Lakshmi JP, Selvi KV. Anticancer potentials of secondary metabolites from endophytes of Barringtonia acutangula and its molecular characterization. Int J Curr Microbiol App Sci 2013;2(2):44-5.

Evidente A, Kornienko A, Cimmino A, Andolfi A, Lefranc F, Mathieu VÌ, et al. Fungal metabolites with anticancer activity. Nat Prod Rep 2014;31:617-27.

Chanda S. Endophytic fungi: novel source of anticancer lead molecules. Appl Microbiol Biotechnol 2012;95:47-59.

Pharamat T, Palaga T, Piapukiew J, Whalley AJ, Sihanonth P. Antimicrobial and anticancer activities of endophytic fungi from Mitragynajavanica Koord and Val. Afr J Microbiol Res 2013;7(49):5565-72.

Tan RX, Zou WH. Endophytes: a rich source of functional metabolites. Nat Prod Rep 2001;18:448-59.

Guo B, Wang Y, Sun X, Tang K. Bioactive natural products from endophytes: a review. Appl Biochem Microbiol 2008;44:136-42.

Selim KA, EL-beih AA, AbdEl-Ranman TM, EL-Diwany AI. Biology of endophytic fungi. Curr Res Environ Appl Mycol 2012;2:31-82.

Published

15-07-2015

How to Cite

Ks, A., and K. Y. L. “CYTOTOXIC ACTIVITY OF ENDOPHYTIC FUNGI HHPCYL03 ISOLATED FROM CYMBOPOGON FLEXUOSUS NEES EX STEUD”. International Journal of Current Pharmaceutical Research, vol. 7, no. 3, July 2015, pp. 70-72, https://mail.innovareacademics.in/journals/index.php/ijcpr/article/view/7929.

Issue

Section

Original Article(s)