A COMPARATIVE STUDY ON IN VITRO ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF METHANOL EXTRACT FROM THE LEAVES OF STACHYTARPHETA INDICA (L) VAHL AND PREMNA CORYMBOSA ROTTL
Keywords:
Stachytarpheta indica, Premna corymbosa, Antioxidant, AntibacterialAbstract
Objective: The objective of this research was to investigate the in vitro antioxidant and antibacterial activities of methanol extract from the leaves of Stachytarpheta indica (L) vahl. and Premna corymbosa Rottl.
Methods: The various photochemicals were analyzed according to the methods described by Allen and Harbone. The total phenolic contents were estimated by Folin–Ciocalteu method. Antioxidant activities of these plants were tested on the basis of ABTS (2,2'-azino-bis-3-ethyl benzthiazoline-6-sulphonic acid) radical scavenging assay, inhibition of lipid peroxidation, Super oxide radical scavenging activity, Nitric oxide radical scavenging activity and Metal chelating activity. Similarly antibacterial activity was performed by Disc diffusion method against Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pnemoniae, Escherichia coli and Enterococcus faecalis.
Results: The phytochemical analysis showed the presence of Tannins, Flavonoids, Alkaloids and Anthraquinones. The total phenolic contents were more in P. corymbosa (381.10±13.00 mg/g) than S. indica (195.72±8.75 mg/g). The methanol extract of the leaves of P. cormbosa showed significant antioxidant activity, while it was comparatively less in S. indica. Similarly the study on antibacterial activity of these two plant leaves extracts revealed inhibitory activity. However P. corymbosa showed higher inhibitory zone against E. coli, K. pneumonia, P. aeroginosa (18, 17, 16 mm) respectively compared with S. indica.
Conclusion: This research work has made it clear that P. corymbosa possess excellent antibacterial and antioxidant activity and the extracts can be more widely used in developing countries for the prevention and treatment of ageing related diseases and may be considered as good source for drug discovery.
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