Hepatoprotective Activity of Different Extracts of Aerial Parts of Limnophilla Indica†by using paracetamol and ethanol induced models
Abstract
The present study was carried out to evaluate the hepatoprotective activity of leaves of Limnophilla indica of family Scrophulariaceae (Dog flower family) .The plant extract was prepared and examined against CCl4 induced liver damage in wistar rats using silymarin as standard. The extract was screened for alkaloids, steroids, proteins, flavanoides, saponins, carbohydrates, tannins and glycosides. Enzyme activities of Serum glutamate oxaloacetate (SGOT), Serum glutamate pyruvate transaminase (SGPT), Alkaline phosphatase, Total bilirubin and Total protein levels are analysed. Results indicate that the methanolic leaf extract had protective activity over paracetamol and ethanol induced hepatotoxicity when compared with toxic control and silymarin groups. Results of present investigation confirm the hepatoprotective activity of Limnophilla indica. The statistical analysis was carried out using one way analysis of varience (ANOVA) followed by Dunnet's t-test. P –values <0.05 were considered as significant.
References
Chopra RN, Nayar SL, Chopra IC, In Glossary of Indian medicinal plants, CSIR, New Delhi, 1st ed, 1956, 197.
Agarwal SS, Singh VK. Immunomodulators: a review of studies on Indian medicinal plants and synthetic peptides. Part I: medicinal plants. Proc. Indian Natl. Sci. Acad.,1999, 65:179-204.
Thatte UM, Dahanukar SA. Rasayana Concept: Clues from immunomodulatory therapy. In: SN UpadhyayEd.Immunomodulation, Narosa Publishing House, NewDelhi.1997, 41-148.
Bhattacharya SK, Bhattacharya A, Chakrabarti A. Adaptogenic activity of Siotone a polyherbal formulation of Ayurvedicrasayanas. Ind. J. Experi. Biol.,2000,38: 119-128.
Ward FM, Daly MJ. Liver disease, In: Roger walker, Clive Edwards, Clinical Pharmacy and Therapeutics, 3rd edition, Churchil Livingstone, New York,2005 209.
BaskarRajan G, Chezhiyan N. Strength and Wealth of Therapeutic medicinal plants in India, In: Irfan A Khan, AtiyaKhanum, Role of Biotechnology in Medicinal and Aromatic plants, Special volume on Diseases, Ukaaz publications, Hyderabad,2002, 6: 151-152.
J.S. Mossa, M. Tariq, A. Mohin, et al. Pharmacological studies on aerial parts of Calotropisprocera.Am J Chin Med,1991, 19: 223-31.
N. Mascola, R. Sharma, S.C. Jain, et al. Ethnopharmacology of Calotropisprocera flowers. J Ethn.pharmacol.,1988,22:211-21.
Mitchell, J.R., Jollow, D.J, Potter, W.Z., Gillette, J.R. and Brodie, B.B. Acetaminophen Induced Hepatic Necrosis. IV. Protective Role of Glutathione,†J. Pharm. Exper. Therapeutics. 187,; 211-217, 1973.
Hinson, J.A., A. B. Reid, Mccullough S.S., and L.P., James. Acetaminophen-Induced Hepatotoxicity: Role of Metabolic Activation, Reactive Oxygen/Nitrogen Species, and Mitochondrial Permeability Transitionâ€, Drug Metabolism Reviwes.; 36, 805-822. 2004
Dahlin D et al. Nacetyl- p-benzoquinone imine: A cytochrome P-450 mediated oxidation product of acetaminophenâ€, Proc. Natl. Acad. Sci.; 81: 1327-1331. 1984
Moron MS, Depierre JW and Mannervik B. Levels of glutathione, glulathione reductase and glutathione-S-transferase activities in rat lung and liverâ€, Biochem. Biophys. Acta.; 582: 67-78. 1979.
Gupta AK et al. Hepatoprotective ativity of Rauwolfia serpentine rhizome in paracetamol intoxicated ratsâ€, J. Pharmacol. Toxicol.; 1: 82-88. 2006.
Shah M et al. Evaluation of the effect of aqueous extract from powders of root, stem, aerial parts and whole plant of Phyllanthus debilis against CCL4 induced rat liver dysfunctionâ€, Indian Drugs.; 39: 333-337. 2002.
Nkosi CZ, Opoku AR and Terblanche SE. Effect of pumpkin seed (Cucurbita pepo) protein isolate on the activity levels of certain plasma enzymes in CCL4 induced injury in low protein fed ratsâ€, Phy. The. Res. 19: 341-345. 2005.
Gupta AK and Misra N. Hepatoprotective Activity of Aqueous Ethanolic Extract of Chamomile capitula in Paracetamol Intoxicated Albino Ratsâ€, American J. Pharm. Toxicol. 1: 17-20. 2006.